High-resolution optical control of spatiotemporal neuronal activity patterns in zebrafish using a digital micromirror device

Zhu, Peixin; Fajardo, Otto; Shum, Jennifer; Schärer, Yan-Ping Zhang; Friedrich, Rainer W.

doi:10.1038/nprot.2012.072

Cited by 115 publications

(134 citation statements)

References 51 publications

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“…In our work, only one aspheric lens with multimode optical fiber makes the system very simple with minimizing power loss only at the cost of lower usable DMD area. And, we were able to achieve high power transmittance (30%) compared to previously reported works (< 10%) [15,22].…”

Section: Discussionsupporting

confidence: 51%

“…Several optical configurations have been implemented as light pattern generator to avoid illumination vignette. Expanded collimation [22], diffuser [23] and beam shaper [24] have been used for light homogenization, but severe power loss limits maximum applicable power density. Fly eye lens makes flat illumination by dividing each spatial beam path, but the lens dimension should be customized to guide light pattern into a microscope.…”

Section: Discussionmentioning

confidence: 99%

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Digital micromirror based near-infrared illumination system for plasmonic photothermal neuromodulation

Jung

Kang

Nam

2017

Biomed. Opt. Express

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Light-mediated neuromodulation techniques provide great advantages to investigate neuroscience due to its high spatial and temporal resolution. To generate a spatial pattern of neural activity, it is necessary to develop a system for patterned-light illumination to a specific area. Digital micromirror device (DMD) based patterned illumination system have been used for neuromodulation due to its simple configuration and design flexibility. In this paper, we developed a patterned near-infrared (NIR) illumination system for region specific photothermal manipulation of neural activity using NIR-sensitive plasmonic gold nanorods (GNRs). The proposed system had high power transmission efficiency for delivering power density up to 19 W/mm 2 . We used a GNR-coated microelectrode array (MEA) to perform biological experiments using E18 rat hippocampal neurons and showed that it was possible to inhibit neural spiking activity of specific area in neural circuits with the patterned NIR illumination. This patterned NIR illumination system can serve as a promising neuromodulation tool to investigate neuroscience in a wide range of physiological and clinical applications. 16 (7), 805-815 (2013). 18. Y. M. Tamar Arens-Arad, N. Faraha, S. Ben-Yaishc, and A. Zlotnikc, "Head mounted DMD based projection system for natural and prosthetic visual stimulation in freely moving rats," Sci.

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Section: Discussionsupporting

confidence: 51%

Section: Discussionmentioning

confidence: 99%

Digital micromirror based near-infrared illumination system for plasmonic photothermal neuromodulation

Jung

Kang

Nam

2017

Biomed. Opt. Express

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“…Early efforts to combine imaging and one-photon (1P) optogenetic manipulation involved the use of nongenetic indicators of activity such as the calcium sensors fura-2 (Zhang et al, 2007) or Fluo-5F (Zhang and Oertner, 2007), or the voltage sensor RH-155 (Airan et al, 2007). This strategy was more recently implemented in vivo to examine the functional properties of interneuron networks (Wilson et al, 2012), map interhemispheric and intrahemispheric connectivity (Lim et al, 2012), and probe motor pattern generation during behavior (Fajardo et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

All-Optical Interrogation of Neural Circuits

et al. 2015

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There have been two recent revolutionary advances in neuroscience: First, genetically encoded activity sensors have brought the goal of optical detection of single action potentials in vivo within reach. Second, optogenetic actuators now allow the activity of neurons to be controlled with millisecond precision. These revolutions have now been combined, together with advanced microscopies, to allow "all-optical" readout and manipulation of activity in neural circuits with single-spike and single-neuron precision. This is a transformational advance that will open new frontiersinneuroscienceresearch.Harnessingthepoweroflightintheall-opticalapproachrequirescoexpressionofgeneticallyencodedactivity sensorsandoptogeneticprobesinthesameneurons,aswellastheabilitytosimultaneouslytargetandrecordthelightfromtheselectedneurons. It has recently become possible to combine sensors and optical strategies that are sufficiently sensitive and cross talk free to enable single-actionpotential sensitivity and precision for both readout and manipulation in the intact brain. The combination of simultaneous readout and manipulationfromthesamegeneticallydefinedcellswillenableawiderangeofnewexperimentsaswellasinspirenewtechnologiesforinteractingwith the brain. The advances described in this review herald a future where the traditional tools used for generations by physiologists to study and interact with the brain-stimulation and recording electrodes-can largely be replaced by light. We outline potential future developments in this field and discuss how the all-optical strategy can be applied to solve fundamental problems in neuroscience.

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“…This interactive system incorporates several recent advances in automated behavior assay systems, including real-time interactive manipulation (16,21,39), wide-field observation of multiple individuals (12)(13)(14), and dual-color lasers for optogenetic manipulation (21). Real-time image analysis and laser tracking modules in ALTOMS offer several advantages: (i) high spatiotemporal precision of laser irradiation of specified body parts in a freely moving fly without disturbing another fly in the same arena, (ii) dual lasers for optogenetic activation or inhibition of selective neural activities simultaneously or separately (21,40), (iii) a flexible and automated training program to manipulate neural circuits involved in courtship learning, and (iv) ready application to the study of other types of social behavior in flies. With the proper initial setup, the use of ALTOMS requires only minimal training without any special prior skills.…”

Section: Discussionmentioning

confidence: 99%

Optogenetic control of selective neural activity in multiple freely moving Drosophila adults

Chu

Hsiao

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

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We present an automated laser tracking and optogenetic manipulation system (ALTOMS) for studying social memory in fruit flies (Drosophila melanogaster). ALTOMS comprises an intelligent central control module for high-speed fly behavior analysis and feedback laser scanning (∼40 frames per second) for targeting two lasers (a 473-nm blue laser and a 593.5-nm yellow laser) independently on any specified body parts of two freely moving Drosophila adults. By using ALTOMS to monitor and compute the locations, orientations, wing postures, and relative distance between two flies in real time and using high-intensity laser irradiation as an aversive stimulus, this laser tracking system can be used for an operant conditioning assay in which a courting male quickly learns and forms a long-lasting memory to stay away from a freely moving virgin female. With the equipped lasers, channelrhodopsin-2 and/or halorhodopsin expressed in selected neurons can be triggered on the basis of interactive behaviors between two flies. Given its capacity for optogenetic manipulation to transiently and independently activate/inactivate selective neurons, ALTOMS offers opportunities to systematically map brain circuits that orchestrate specific Drosophila behaviors.operant learning | restraining order | restraining conditioning S ocial interactions are an important part of human life because they help us learn how to behave in a society. However, the mechanisms by which the neuron circuitry controls and modifies our behavior on the basis of previous experiences of interactions with others remain unclear. Drosophila courtship conditioning has been widely used for studying how genes and brain circuits control and modify a specific type of social interaction (1-6). In this behavioral assay, individual male fruit flies learn to suppress their courtship activity after several hours of exposure to an unreceptive female. Specific cuticular pheromones, such as 9-pentacosene, have been shown to potentially serve as conditioned stimuli (4, 7-9). Visual inputs act as conditioned stimuli for courtship through modulation of Ca 2+ /calmodulin-dependent protein kinase activity in the brain circuitry (4). An aversive male pheromone, cis-vaccenyl acetate, which is transferred to the female during copulation, may act as a punishment so that the rejected male forms a generalized memory that suppresses its subsequent courtship behavior (10). However, little is known about where and how the neural activities that represent the antecedent conditions and aversive consequence are associated in the brain-knowledge that is crucial for understanding courtship memory and decision making-because controlling female rejection behaviors (11) and acutely manipulating target neurons in courting males during social interaction are difficult. Here, we present an automated laser tracking system for real-time analysis and perturbation of social interactions between two freely moving adult flies that is equipped with highenergy laser irradiation as a controllable punishment source ...

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High-resolution optical control of spatiotemporal neuronal activity patterns in zebrafish using a digital micromirror device

Cited by 115 publications

References 51 publications

Digital micromirror based near-infrared illumination system for plasmonic photothermal neuromodulation

Digital micromirror based near-infrared illumination system for plasmonic photothermal neuromodulation

All-Optical Interrogation of Neural Circuits

Optogenetic control of selective neural activity in multiple freely moving Drosophila adults

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