Light field imaging (LFI) based on Liquid crystal microlens array (LC MLAs) are emerging as a significant area for 3D imaging technology in the field of upcoming Internet of things and artificial intelligence era. However, in scenes of LFI through conventional MLAs, such as biological imaging and medicine imaging, the quality of imaging reconstruction will be severely reduced due to the limited depth of field. Here, we are proposed a low-voltage driving LC MLAs with electrically tunable depth of field (DOF) for the LFI system. An aluminum-doped zinc oxide (AZO) film was deposited on the top of the hole-patterned driven-electrode arrays and used as a high resistance (Hi-R) layer, a uniform gradient electric field was obtained across the sandwiched LC cell. Experimental results confirm that the proposed LC MLAs possess high-quality interference rings and tunable focal length at a lower working voltage. In addition, the focal lengths are tunable from 3.93 to 2.62 mm and the DOF are adjustable from 15.60 to 1.23 mm. The experiments demonstrated that the LFI system based on the proposed structure can clearly capture 3D information of the insets with enlarged depths by changing the working voltage and driving frequency, which indicates that the tunable DOF LC MLAs have a potential application prospects for the biological and medical imaging.