High resolution proton NMR investigations of rat blood plasma Assignment of resonances for the molecularly mobile carbohydrate side‐chains of ‘acute‐phase’ glycoproteins

Grootveld, Martin; Claxson, Andrew W.D.; Chander, C. L.; Haycock, Peter R.; Blake, David R.; Hawkes, Geoffrey E.

doi:10.1016/0014-5793(93)81584-m

Cited by 33 publications

(28 citation statements)

References 32 publications

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“…Phophocholine is an abundant structural component of the cell membrane [47], [48]. Serum O-acetyl-glycoprotein is an “acute phase” glycoprotein that is associated with inflammation of injury tissue in inflammatory animal models [47], [49]. Previous studies have suggested that elevated O-acetyl glycoprotein fragment signals in the blood are associated with inflammatory associated diseases, including cancer, certain liver diseases, and also surgical trauma [49], [50].…”

Section: Resultsmentioning

confidence: 99%

“…Serum O-acetyl-glycoprotein is an “acute phase” glycoprotein that is associated with inflammation of injury tissue in inflammatory animal models [47], [49]. Previous studies have suggested that elevated O-acetyl glycoprotein fragment signals in the blood are associated with inflammatory associated diseases, including cancer, certain liver diseases, and also surgical trauma [49], [50]. GA was able to reduce these increased levels of metabolites, which indicates that GA ameliorates hepatosteatosis and protects the liver against injury during HFD-feeding ( Figure 1D and Figure 2E ).…”

Section: Resultsmentioning

confidence: 99%

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Gallic Acid Ameliorated Impaired Glucose and Lipid Homeostasis in High Fat Diet-Induced NAFLD Mice

Chao¹,

Huo

Cheng³

et al. 2014

PLoS ONE

139

100

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Gallic acid (GA), a naturally abundant plant phenolic compound in vegetables and fruits, has been shown to have potent anti-oxidative and anti-obesity activity. However, the effects of GA on nonalcoholic fatty liver disease (NAFLD) are poorly understood. In this study, we investigated the beneficial effects of GA administration on nutritional hepatosteatosis model by a more “holistic view” approach, namely 1H NMR-based metabolomics, in order to prove efficacy and to obtain information that might lead to a better understanding of the mode of action of GA. Male C57BL/6 mice were placed for 16 weeks on either a normal chow diet, a high fat diet (HFD, 60%), or a high fat diet supplemented with GA (50 and 100 mg/kg/day, orally). Liver histopathology and serum biochemical examinations indicated that the daily administration of GA protects against hepatic steatosis, obesity, hypercholesterolemia, and insulin resistance among the HFD-induced NAFLD mice. In addition, partial least squares discriminant analysis scores plots demonstrated that the cluster of HFD fed mice is clearly separated from the normal group mice plots, indicating that the metabolic characteristics of these two groups are distinctively different. Specifically, the GA-treated mice are located closer to the normal group of mice, indicating that the HFD-induced disturbances to the metabolic profile were partially reversed by GA treatment. Our results show that the hepatoprotective effect of GA occurs in part through a reversing of the HFD caused disturbances to a range of metabolic pathways, including lipid metabolism, glucose metabolism (glycolysis and gluconeogenesis), amino acids metabolism, choline metabolism and gut-microbiota-associated metabolism. Taken together, this study suggested that a 1H NMR-based metabolomics approach is a useful platform for natural product functional evaluation. The selected metabolites are potentially useful as preventive action biomarkers and could also be used to help our further understanding of the effect of GA in hepatosteatosis mice.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Gallic Acid Ameliorated Impaired Glucose and Lipid Homeostasis in High Fat Diet-Induced NAFLD Mice

Chao¹,

Huo

Cheng³

et al. 2014

PLoS ONE

139

100

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“…In the current study, increased intensity of O-acetyl glycoprotein fragment resonance was observed in infected mice. Elevated O-acetyl glycoprotein fragment signals from acute phase reactive protein in rat blood plasma was considered to be associated with inflammatory conditions, such as cancer, certain liver diseases, and also during surgical trauma (33,34).…”

Section: Discussionmentioning

confidence: 99%

Global metabolic responses of mice to Trypanosoma brucei brucei infection

Wang

Utzinger²,

Saric

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

126

185

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Human African trypanosomiasis (HAT) is transmitted by tsetse flies and, if untreated, is fatal. Treatment depends on infection stage, and early diagnosis is crucial for effective disease management. The systemic host biochemical changes induced by HAT that enable biomarker discovery or relate to therapeutic outcome are largely unknown. We have characterized the multivariate temporal responses of mice to Trypanosoma brucei brucei infection, using 1 H nuclear magnetic resonance (NMR) spectroscopic metabolic phenotyping of urine and plasma. Marked alterations in plasma metabolic profiles were detected already 1 day postinfection. Elevated plasma concentrations of lactate, branched chain amino acids, and acetylglycoprotein fragments were noted. T. brucei brucei-infected mice also had an imbalance of plasma alanine and valine, consistent with differential gluconeogenesis (parasite)-ketogenesis (host) pathway counterflux, involving stimulated host glycolysis, ketogenesis, and enhanced lipid oxidation in the host. Histopathologic evidence of T. brucei brucei-induced extramedullary hepatic hemopoiesis, renal interstitial nephritis, and a provoked inflammatory response was also noted. Metabolic disturbance of gut microbiotal activity was associated with infection, as indicated by changes in the urinary concentrations of the microbial co-metabolites, including hippurate. Concluding, parasite infection results in multiple systemic biochemical effects in the host and disturbance of the symbiotic gut microbial metabolic interactions. Investigation of these transgenomic metabolic alterations may underpin the development of new diagnostic criteria and metrics of therapeutic efficacy. diagnosis ͉ metabonomics ͉ NMR spectroscopy ͉ trypanosomiasis

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“…"Acute-phase" glycoproteins appeared as broad signals at 2.14 and 2.05 parts per million (Figure 4). The signal at 2.14 parts per million was assigned by Grootveld et al (1993) to O-acetylsialate sugars, mainly from α1-acid glycoprotein, whereas the signal at 2.05 parts per million was assigned to N-acetyl groups from "acute-phase" glycoproteins (Bell et al 1987).…”

Section: Metabolites Associated With Caloric Restrictionmentioning

confidence: 99%

“…Spectral signals were assigned by comparison of chemical shifts with values from the literature (Bell et al 1987;Grootveld et al 1993;Nicholson et al 1995;Liu et al 2002) and from the Chenomx library (Chenomx NMR Suite 7.5, Chenomx Inc., Alberta, US). In order to support the assignment of metabolite signals, 1 H, 1 H-TOCSY (total correlation spectroscopy) and 1 H, 13 C-HSQC (heteronuclear single-quantum coherence) were run on selected samples, which were then fortified with expected metabolites.…”

Section: Measurementsmentioning

confidence: 99%

Nuclear Magnetic Resonance-Based Blood Metabolic Profiles of Rats Exposed to Short-Term Caloric Restriction

et al. 2015

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Caloric restriction increases life-span of a number of organisms. The relationship between the increase in life-span and the extent of caloric restriction, however, varies among species. The underlying mechanisms are yet unknown, but appear to be related to changes in metabolism. In order to investigate the metabolic response of caloric restriction of rats, here is presented the first nuclear magnetic resonance (NMR) spectroscopy-based study of how blood metabolite profiles are influenced by graded levels of caloric restriction. The study involved three groups of obese Downloaded by [New York University] at 00:38 06 July 2015 2 rats exposed to 0, 20, and 40 percent caloric restriction for five days. Blood serum from each individual was analyzed by 1 H NMR and the resulting spectra were subjected to multivariate analysis by unsupervised principal component analysis and supervised orthogonal-partial least square discriminant analysis. The analyzes revealed that a response to caloric restriction was present at 20 percent caloric restriction. The metabolites that distinguished the profiles at 20 percent restriction deviated from those at 40 percent restriction. The changes induced by caloric restriction were most clearly observed as an increased level of 3-hydroxybutyrate, and decreased levels of lipids and pyruvate. The metabolic responses of rats exposed to caloric restriction are in good agreement with a switch in metabolism from anabolic pathways towards fatty acid catabolism and gluconeogenesis, which is consistent with previous observations for mice.

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High resolution proton NMR investigations of rat blood plasma Assignment of resonances for the molecularly mobile carbohydrate side‐chains of ‘acute‐phase’ glycoproteins

Cited by 33 publications

References 32 publications

Gallic Acid Ameliorated Impaired Glucose and Lipid Homeostasis in High Fat Diet-Induced NAFLD Mice

Gallic Acid Ameliorated Impaired Glucose and Lipid Homeostasis in High Fat Diet-Induced NAFLD Mice

Global metabolic responses of mice to Trypanosoma brucei brucei infection

Nuclear Magnetic Resonance-Based Blood Metabolic Profiles of Rats Exposed to Short-Term Caloric Restriction

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