High-resolution proton nuclear magnetic resonance: application to the study of leukaemic lymphocytes

Mountford, C.E.; Grossman, George; Gatenby, Piers; Fox, Richard M.

doi:10.1038/bjc.1980.182

Cited by 24 publications

(8 citation statements)

References 4 publications

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“…The observed narrowing of the composite lipid CH2 resonance (18) could be due (a) to a true increase in T2 for one or more signal components as recently reported for membrane components of metastasizing tumor cells (19)(20)(21)(22), or for a proteolipid fraction present in plasma from patients with malignant disease (23, 24), or (b) to a change to a more homogeneous lipid/lipoprotein composition that could give line narrowing without increasing T2. A true increase in T2 can be caused by a reduction in the molecular weight of lipoprotein complexes, or an increase in the mobility/ fluidity of triglycerides within such complexes.…”

Section: Discussionsupporting

confidence: 53%

NMR relaxation times T₁ and T² of water in plasma from patients with lung carcinoma: Correlation of T² with blood sedimentation rate

Schuhmacher

Clorius

Semmler³

et al. 1987

Magnetic Resonance in Med

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The T1 and T2 NMR relaxation times of water protons in plasma, obtained from patients with lung carcinoma, healthy volunteers, and patients with nontumor diseases were measured at a resonance frequency of 20 MHz. Additionally hematologic parameters were determined. In tumor patients the mean plasma T2 was shortened by about 20%, and an increase in the blood sedimentation rate (BSR) was noted. Similar but less pronounced results were found for the nontumor group of patients, indicating that the shortening of T2 in plasma is a secondary host response. However, a plot of plasma 1/T2 versus BSR from tumor patients showed a significant correlation between these two parameters. No such correlation could be detected in the nontumor group of patients. The correlation of 1/T2 with BSR, found solely in the tumor patient group, increased the diagnostic sensitivity of T2 measurements and may help to differentiate between malignant and nonmalignant disease. No significant variation in the T1 spin-lattice relaxation time was observed.

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Section: Discussionsupporting

confidence: 53%

NMR relaxation times T₁ and T² of water in plasma from patients with lung carcinoma: Correlation of T² with blood sedimentation rate

Schuhmacher

Clorius

Semmler³

et al. 1987

Magnetic Resonance in Med

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“…The cellular integrity may be maintained for a sufficient period of time to acquire 1D and 2D spectra if the cell density is adequate (around 10 8 cells per mL, depending on field strength). Data from such cell samples were first obtained by the C. Mountford group [43]. The concept of "mobile lipids", i.e.…”

Section: Ii-a-experimental Conditions For Nmr Spectroscopy Of Cellsmentioning

confidence: 99%

Nuclear Magnetic Resonance Spectroscopy of Lipids in Cancer

Certaines

Moyec²,

Séguin³

et al. 2007

COC

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Analytical methods often benefit from progress in other fields of science. One of the best-illustrated and most spectacular examples is NMR technology and its numerous improvements that have resulted from new applications in biology and medicine. These techniques came under the term MRI during the mid 1970s, and then in vivo MRS since the early 1980s. Biomedical NMR of lipids is presently emerging as an exciting topic in onco-biology. However, for various reasons, biochemists up to quite recently showed more interest in proteins than in lipids. This is probably because proteins are "active" reactant molecules and lipids are often still considered as merely nutritional or structurally more "passive" components. While classical biochemistry quantifies molecules independently from each other, biophysical methods, including NMR, also take into account the multi-molecular structure and the resulting "visibility" of each component, which is itself related to mobility. Results from numerous studies concerning lipids in cancer mainly focused on blood plasma using high resolution in vitro NMR, cells using both classical high resolution ex-vivo NMR and high-resolution Magic Angle Spinning NMR and, more recently, in vivo tumours by single-voxel spectroscopy or by Chemical Shift Imaging.

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“…The examination, by MRS, of excised intact thymus from an inbred strain of mice (6) indicated that the chemical composition of the thymus cells changed prior to such changes being identifiable cytologically or histologically. Thus MRS measured a continuum of changes taking place in the thymus as the final preleukemic period ended and neoplasia occurred (7, 8).…”

Section: The Early History Of Proton Mrsmentioning

confidence: 99%

Proton spectroscopy provides accurate pathology on biopsy and in vivo

Mountford

Lean

Malycha

et al. 2006

Magnetic Resonance Imaging

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In the last 25 years, MR spectroscopy (MRS) has moved from being a basic research tool into routine clinical use. The spectroscopy method reports on those chemicals that are mobile on the MR time scale. Many of these chemicals reflect specific pathological processes but are complicated by the fact that many chemicals change at one time. There are currently two clinical applications for spectroscopy. The first is in the pathology laboratory, where it can be an adjunct to, and in some cases replacement, for difficult pathologies like Barrett's esophagus and follicular adenoma of the thyroid. The spectroscopy method on a breast biopsy can also report on prognostic indicators, including the potential for spread, from information present in the primary tumor alone. The second application for spectroscopy is in vivo to provide a preoperative diagnosis and this is now achievable for several organs including the prostate. The development of spectroscopy for clinical purposes has relied heavily on the serially-sectioned histopathology to confirm the high accuracy of the method. The combination of in vivo MRI, in vivo MRS, and ex vivo MRS on biopsy samples offers a modality of very high accuracy for preoperative diagnosis and provision of prognostic information for human cancers.

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High-resolution proton nuclear magnetic resonance: application to the study of leukaemic lymphocytes

Cited by 24 publications

References 4 publications

NMR relaxation times T₁ and T² of water in plasma from patients with lung carcinoma: Correlation of T² with blood sedimentation rate

NMR relaxation times T₁ and T² of water in plasma from patients with lung carcinoma: Correlation of T² with blood sedimentation rate

Nuclear Magnetic Resonance Spectroscopy of Lipids in Cancer

Proton spectroscopy provides accurate pathology on biopsy and in vivo

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High-resolution proton nuclear magnetic resonance: application to the study of leukaemic lymphocytes

Cited by 24 publications

References 4 publications

NMR relaxation times T1 and T2 of water in plasma from patients with lung carcinoma: Correlation of T2 with blood sedimentation rate

NMR relaxation times T1 and T2 of water in plasma from patients with lung carcinoma: Correlation of T2 with blood sedimentation rate

Nuclear Magnetic Resonance Spectroscopy of Lipids in Cancer

Proton spectroscopy provides accurate pathology on biopsy and in vivo

Contact Info

Product

Resources

About

NMR relaxation times T₁ and T² of water in plasma from patients with lung carcinoma: Correlation of T² with blood sedimentation rate

NMR relaxation times T₁ and T² of water in plasma from patients with lung carcinoma: Correlation of T² with blood sedimentation rate