High‐resolution quantitative acoustic microscopy of cutaneous carcinoma and melanoma: Comparison with histology

Abstract

Background The increased incidence rate of skin cancers during the last decades is alarming. One of the significant difficulties in the histopathology of skin cancers is appearance variability due to the heterogeneity of diseases or tissue preparation and staining process. This study aims to investigate whether the high‐resolution acoustic microscopy has the potential for identifying and quantitatively classifying skin cancers. Material/Methods Unstained standard formalin‐fixed skin tissue samples were used for ultrasonic examination. The high‐frequency acoustic microscope equipped with the 320 MHz transducer was utilized to visualize skin structure. Fourier transform was performed to calculate the sound speed and attenuation in the tissue. Results The acoustic images demonstrate good concordance with the traditional histology images. All histological features in the tumour were easily identifiable on acoustic images. Each skin cancer type has its combination of ultrasonic properties significantly different from the healthy skin. Conclusions High‐resolution acoustic imaging strengthened with quantitative analysis shows a potential to work as an auxiliary imaging modality assisting pathologists to lean to the particular decision in doubtful cases. The method can also assist surgeon to ensure the complete resection of a tumour.

The Reference Phase Correction for the Fluctuated Scanning Lines and the Slope of the Stage in Tissue Characterization by Scanning Acoustic Microscope

“…The average values of acoustic velocity from different regions are demonstrated in Table 1 and Figure S5. Similar results have been suggested by Youssef et al [23], indicating that the melanoma cells demonstrate the lowest values of sound speed (1360 ± 50 m/s). Figure 6.…”

Effects of radiation exposure on dermal collagen: A multi modal approach

“…Recent advancements in Scanning Acoustic Microscopy (SAM) has induced considerable interest to this method for assessment of both histological and elastic properties using the same tissue section 13,14 . This technique demonstrates great concordance with histology analysis based on light microscopy 15,16 . This study investigates the physical, chemical and mechanical effects of IPL and radiation on collagen fibrils in the skin.…”