High-resolution-scanning waveguide microscopy: spatial refractive index and topography quantification

Berguiga, Lotfi; Orobtchouk, Régis; Elezgaray, Juan; Arnéodo, A.; Argoul, Françoise

doi:10.1364/ol.42.002523

Cited by 3 publications

(3 citation statements)

References 15 publications

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“…In recent years, surface plasmon resonance microscopy (SPRM) has developed tremendously. − It has been used to study the cell–substrate adhesion ,, as well as dynamic cellular processes. ,, …”

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confidence: 99%

Nanometer-Resolved Mapping of Cell–Substrate Distances of Contracting Cardiomyocytes Using Surface Plasmon Resonance Microscopy

et al. 2018

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It has been shown that quantitative measurements of the cell-substrate distance of steady cells are possible with scanning surface plasmon resonance microscopy setups in combination with an angle resolved analysis. However, the accuracy of the determined cell-substrate distances as well as the capabilities for the investigation of cell dynamics remained limited due to the assumption of a homogeneous refractive index of the cytosol. Strong spatial or temporal deviations between the local refractive index and the average value can result in errors in the calculated cell-substrate distance of around 100 nm, while the average accuracy was determined to 37 nm. Here, we present a combination of acquisition and analysis techniques that enables the measurement of the cell-substrate distance of contractile cells as well as the study of intracellular processes through changes in the refractive index at the diffraction limit. By decoupling the measurement of the cell-substrate distance and the refractive index of the cytoplasm, we could increase the accuracy of the distance measurement on average by a factor of 25 reaching 1.5 nm under ideal conditions. We show a temporal and spatial mapping of changes in the refractive index and the cell-substrate distance which strongly correlate with the action potentials and reconstruct the three-dimensional profile of the basal cell membrane and its dynamics, while we reached an actual measurement accuracy of 2.3 nm.

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confidence: 99%

Nanometer-Resolved Mapping of Cell–Substrate Distances of Contracting Cardiomyocytes Using Surface Plasmon Resonance Microscopy

et al. 2018

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“…In order to improve the image contrast, M. G. Somekh et al introduced a Z-direction movement mechanism to the two-dimensional scanning mechanism. They found that if the sample is slightly shifted from the focus by about 1 µm, the image contrast (refractive index sensitivity) will be improved and the refractive index can be retrieved from the interference fringes [106,107]. In 2012, B. Zhang et al proposed a confocal system integrated scanning SPM, which provides a simpler and more stable alternative [108].…”

Section: Scanning Localized Surface Plasmon Microscopy (Slspm)mentioning

confidence: 99%

“…In 2012, B. Zhang et al proposed a confocal system integrated scanning SPM, which provides a simpler and more stable alternative [108]. L. Berguiga et al reported a high-resolution metal-clad waveguide scanning microscopy with a diffraction-limited resolution, which can be operated in both TM and TE waveguide modes with radially and azimuthally polarized beams, respectively, and allows both refractive index and topography of dielectric objects to be evaluated at high resolution and sensitivity [106]. K. Watanabe reported a high resolution imaging with lateral resolution of~170 nm and thickness resolution against the deposited lipid bilayer being~0.33 nm [109].…”

Section: Scanning Localized Surface Plasmon Microscopy (Slspm)mentioning

confidence: 99%

Advanced Label-Free Laser Scanning Microscopy and Its Biological Imaging Application

Wang

Huang

2021

Applied Sciences

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By eliminating the photodamage and photobleaching induced by high intensity laser and fluorescent molecular, the label-free laser scanning microscopy shows powerful capability for imaging and dynamic tracing to biological tissues and cells. In this review, three types of label-free laser scanning microscopies: laser scanning coherent Raman scattering microscopy, second harmonic generation microscopy and scanning localized surface plasmon microscopy are discussed with their fundamentals, features and recent progress. The applications of label-free biological imaging of these laser scanning microscopies are also introduced. Finally, the performance of the microscopies is compared and the limitation and perspectives are summarized.

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Pupil function engineering for surface plasmon microscopic interferometry

Zhang

2020

Optics & Laser Technology

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High-resolution-scanning waveguide microscopy: spatial refractive index and topography quantification

Cited by 3 publications

References 15 publications

Nanometer-Resolved Mapping of Cell–Substrate Distances of Contracting Cardiomyocytes Using Surface Plasmon Resonance Microscopy

Nanometer-Resolved Mapping of Cell–Substrate Distances of Contracting Cardiomyocytes Using Surface Plasmon Resonance Microscopy

Advanced Label-Free Laser Scanning Microscopy and Its Biological Imaging Application

Pupil function engineering for surface plasmon microscopic interferometry

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