High-resolution short-exposure small-animal laboratory x-ray phase-contrast tomography

Larsson, Daniel H.; Vågberg, William; Yaroshenko, Andre; Yildirim, Ali Önder; Hertz, Hans M.

doi:10.1038/srep39074

Cited by 46 publications

(30 citation statements)

References 36 publications

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“…X-ray based ex vivo CT is considered the most powerful technique to provide high spatial resolution of small structures (Liguori et al, 2015;Larsson et al, 2016;Mortensen et al, 2018;Litmanovich et al, 2019). Here we use µ-CT, which is capable of volumetric analysis with a voxel size <20 µm (Holdsworth et al, 2002;Clark et al, 2014;Senter-Zapata et al, 2016) to characterize the 3D morphology of the cardiac compartments and adjacent vessels.…”

Section: Micro-ct Analyses Reveal Outflow Tract Defects In Alk5a Mutantsmentioning

confidence: 99%

Integration of multiple imaging platforms to uncover cardiac defects in adult zebrafish

Bensimon-Brito

Boezio

Cardeira-da-Silva

et al. 2020

Preprint

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Mammalian models have been instrumental to investigate adult heart function and human disease. However, electrophysiological differences with human hearts and high costs emphasize the need for additional models. The zebrafish is a well-established genetic model to study cardiac development and function; however, analysis of cardiac phenotypes in adult specimens is particularly challenging as they are opaque. Here, we optimized and combined multiple imaging techniques including echocardiography, magnetic resonance imaging and micro-computed tomography to identify and analyze cardiac phenotypes in adult zebrafish. Using alk5a/tgfbr1a mutants as a case study, we observed morphological and functional cardiac defects, which were undetected with conventional approaches. Correlation analysis of multiple parameters revealed an association between hemodynamic defects and structural alterations of the heart, as observed clinically. Thus, we report a comprehensive and sensitive platform to identify otherwise indiscernible cardiac phenotypes in adult zebrafish, a model with clear advantages to study cardiac function and disease.

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Section: Micro-ct Analyses Reveal Outflow Tract Defects In Alk5a Mutantsmentioning

confidence: 99%

Integration of multiple imaging platforms to uncover cardiac defects in adult zebrafish

Bensimon-Brito

Boezio

Cardeira-da-Silva

et al. 2020

Preprint

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“…A C C E P T E D M A N U S C R I P T 43,44], mean that the widespread use of dynamic propagation-based imaging is moving closer to a reality. For example, Larsson et al used a liquid-metal-jet x-ray source to capture phase-contrast tomographic images of an excised air-filled mouse lung [45], Preissner et al captured in vivo mouse lung structure [46], and Kim et al performed 4DCT measurements at a liquid-metal-jet source to explore the effect of mechanical ventilation on the lung [47]. Using an inverse Compton source, we have recently shown in vivo PCXI of mouse respiratory function [48].…”

Section: Accepted Manuscriptmentioning

confidence: 99%

Visualizing treatment delivery and deposition in mouse lungs using in vivo x-ray imaging

Gradl

Dierolf

Yang³

et al. 2019

Journal of Controlled Release

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The complexity of lung diseases makes pre-clinical in vivo respiratory research in mouse lungs of great importance for a better understanding of physiology and therapeutic effects. Synchrotron-based imaging has been successfully applied to lung research studies, however longitudinal studies can be difficult to perform due to limited facility access. Laboratory-based x-ray sources, such as inverse Compton x-ray sources, remove this access limitation and open up new possibilities for pre-clinical small-animal lung research at high spatial and temporal resolution. The in vivo visualization of drug deposition in mouse lungs is of interest, particularly in longitudinal research, because the therapeutic outcome is not only dependent on the delivered dose of the drug, but also on the spatial distribution of the drug. An additional advantage of this approach, when compared to other imaging techniques, is that anatomic and dynamic information is collected simultaneously. Here we report the use of dynamic x-ray phase-contrast imaging to observe pulmonary drug delivery via liquid instillation, and by inhalation of micro-droplets. Different liquid volumes (4 l  , 20 l  , 50 l ) were tested and a range of localized and global distributions were observed with a temporal resolution of up to 1.5 fps. The in vivo imaging results were confirmed by ex vivo x-ray and fluorescence imaging. This ability to visualize pulmonary substance deposition in live small animals has provided a better understanding of the two key methods of delivery; instillation and nebulization.

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“…Secondly, full-field x-ray imaging is of tremendous practical importance, in particular for larger specimen and for tomography, where scanning techniques are prohibitive. Importantly, near-field or propagation imaging is almost always known to be implemented in a partial-coherence setting, including robust exploitation of phase contrast at laboratory sources [56,[119][120][121].…”

Section: Introductionmentioning

confidence: 99%

X-Ray Near-Field Holography: Beyond Idealized Assumptions of the Probe

Hagemann¹

2017

Göttingen Series in X-Ray Physics

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High-resolution short-exposure small-animal laboratory x-ray phase-contrast tomography

Cited by 46 publications

References 36 publications

Integration of multiple imaging platforms to uncover cardiac defects in adult zebrafish

Integration of multiple imaging platforms to uncover cardiac defects in adult zebrafish

Visualizing treatment delivery and deposition in mouse lungs using in vivo x-ray imaging

X-Ray Near-Field Holography: Beyond Idealized Assumptions of the Probe

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