High resolution typing of Chlamydophila psittaci by multilocus VNTR analysis (MLVA)

Laroucau, Karine; Thierry, Simon; Vorimore, Fabien; Blanco, Kinndle; Kaleta, E. F.; Hoop, Richard; Magnino, Simone; Vanrompay, Daisy; Sachse, Konrad; Myers, Garry; Bavoil, Patrik M.; Vergnaud, Gilles; Pourcel, Christine

doi:10.1016/j.meegid.2007.12.002

Cited by 37 publications

(19 citation statements)

References 24 publications

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“…This identified association between C. psittaci genotype and host species has not been previously observed, which may due to the difference in sequences studied. Cluster analyses of C. psittaci has previously been performed using ompA [50], [51], ompA based PCR-RFLP [52] or multi loci variable number of tandem repeats (MLVA) [53]. ompA encodes the major outer membrane protein and is subjected to host immuno-pressure and its sequence may therefore not reflect the genetic make up of Chlamydia .…”

Section: Discussionmentioning

confidence: 99%

Multi Locus Sequence Typing of Chlamydia Reveals an Association between Chlamydia psittaci Genotypes and Host Species

et al. 2010

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Chlamydia comprises a group of obligate intracellular bacterial parasites responsible for a variety of diseases in humans and animals, including several zoonoses. Chlamydia trachomatis causes diseases such as trachoma, urogenital infection and lymphogranuloma venereum with severe morbidity. Chlamydia pneumoniae is a common cause of community-acquired respiratory tract infections. Chlamydia psittaci, causing zoonotic pneumonia in humans, is usually hosted by birds, while Chlamydia abortus, causing abortion and fetal death in mammals, including humans, is mainly hosted by goats and sheep. We used multi-locus sequence typing to asses the population structure of Chlamydia. In total, 132 Chlamydia isolates were analyzed, including 60 C. trachomatis, 18 C. pneumoniae, 16 C. abortus, 34 C. psittaci and one of each of C. pecorum, C. caviae, C. muridarum and C. felis. Cluster analyses utilizing the Neighbour-Joining algorithm with the maximum composite likelihood model of concatenated sequences of 7 housekeeping fragments showed that C. psittaci 84/2334 isolated from a parrot grouped together with the C. abortus isolates from goats and sheep. Cluster analyses of the individual alleles showed that in all instances C. psittaci 84/2334 formed one group with C. abortus. Moving 84/2334 from the C. psittaci group to the C. abortus group resulted in a significant increase in the number of fixed differences and elimination of the number of shared mutations between C. psittaci and C. abortus. C. psittaci M56 from a muskrat branched separately from the main group of C. psittaci isolates. C. psittaci genotypes appeared to be associated with host species. The phylogentic tree of C. psittaci did not follow that of its host bird species, suggesting host species jumps. In conclusion, we report for the first time an association between C. psittaci genotypes with host species.

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Section: Discussionmentioning

confidence: 99%

Multi Locus Sequence Typing of Chlamydia Reveals an Association between Chlamydia psittaci Genotypes and Host Species

et al. 2010

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“…psittaci and C . abortus shows a high discrimination power, although the number of repeats does not vary greatly between strains (2–6 copies) [42, 43]. …”

Section: Geneticsmentioning

confidence: 99%

Recent advances in the understanding ofChlamydophila pecoruminfections, sixteen years after it was named as the fourth species of the Chlamydiaceae family

Mohamad

Rodolakis

2009

Vet. Res.

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Chlamydophila pecorum found in the intestine and vaginal mucus of asymptomatic ruminants has also been associated with different pathological conditions in ruminants, swine and koalas. Some endangered species such as water buffalos and bandicoots have also been found to be infected by C. pecorum. The persistence of C. pecorum strains in the intestine and vaginal mucus of ruminants could cause long-term sub-clinical infection affecting the animal’s health. C. pecorum strains present many genetic and antigenic variations, but coding tandem repeats have recently been found in some C. pecorum genes, allowing C. pecorum strains isolated from sick animals to be differentiated from those isolated from asymptomatic animals. This review provides an update on C. pecorum infections in different animal hosts and the implications for animal health. The taxonomy, typing and genetic aspects of C. pecorum are also reviewed.

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“…Meanwhile, genotyping based on PCR and sequencing of ompA has gradually replaced serotyping. Indeed, several new methods were proposed, such as DNA microarray testing [16], multi-locus sequence typing (MLST) [17] and typing based on variable number tandem repeats (VNTR) [18]. However, none of these methods is congruent with the virulence of chlamydial isolates, although some parameters are correlated with clinical manifestations and serotyping.…”

Section: Introductionmentioning

confidence: 99%

Host Adaptation of Chlamydia pecorum towards Low Virulence Evident in Co-Evolution of the ompA, incA, and ORF663 Loci

et al. 2014

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Chlamydia (C.) pecorum, an obligate intracellular bacterium, may cause severe diseases in ruminants, swine and koalas, although asymptomatic infections are the norm. Recently, we identified genetic polymorphisms in the ompA, incA and ORF663 genes that potentially differentiate between high-virulence C. pecorum isolates from diseased animals and low-virulence isolates from asymptomatic animals. Here, we expand these findings by including additional ruminant, swine, and koala strains. Coding tandem repeats (CTRs) at the incA locus encoded a variable number of repeats of APA or AGA amino acid motifs. Addition of any non-APA/AGA repeat motif, such as APEVPA, APAVPA, APE, or APAPE, associated with low virulence (P<10−4), as did a high number of amino acids in all incA CTRs (P = 0.0028). In ORF663, high numbers of 15-mer CTRs correlated with low virulence (P = 0.0001). Correction for ompA phylogram position in ORF663 and incA abolished the correlation between genetic changes and virulence, demonstrating co-evolution of ompA, incA, and ORF663 towards low virulence. Pairwise divergence of ompA, incA, and ORF663 among isolates from healthy animals was significantly higher than among strains isolated from diseased animals (P≤10−5), confirming the longer evolutionary path traversed by low-virulence strains. All three markers combined identified 43 unique strains and 4 pairs of identical strains among all 57 isolates tested, demonstrating the suitability of these markers for epidemiological investigations.

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High resolution typing of Chlamydophila psittaci by multilocus VNTR analysis (MLVA)

Cited by 37 publications

References 24 publications

Multi Locus Sequence Typing of Chlamydia Reveals an Association between Chlamydia psittaci Genotypes and Host Species

Multi Locus Sequence Typing of Chlamydia Reveals an Association between Chlamydia psittaci Genotypes and Host Species

Recent advances in the understanding ofChlamydophila pecoruminfections, sixteen years after it was named as the fourth species of the Chlamydiaceae family

Host Adaptation of Chlamydia pecorum towards Low Virulence Evident in Co-Evolution of the ompA, incA, and ORF663 Loci

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