High-resolution wide-field human brain tumor margin detection and in vivo murine neuroimaging

Liba, Orly; SoRelle, Elliott D.; Dutta, Rebecca; Yuan, Edwin; Vogel, Hannes; Grant, Gerald A.; Zerda, Adam de la

doi:10.1101/252080

Cited by 3 publications

(2 citation statements)

References 55 publications

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“…The marriage of modern AO and ultrahigh speed OCT scan technologies should enable routine implementation of APM-AO-OCT in clinical and research settings, enhancing cellular resolution clinical diagnosis as well as basic science discovery. For example, implementation of APM-AO-OCT should lead to broader application of OCT to brain structural imaging [68,69] where the presence of speckle in images remains a major obstacle. It may also help to suppress the speckle introduced by OCT contrast agents, such as gold nanoparticles [70][71][72].…”

Section: Future Directionsmentioning

confidence: 99%

Aperture phase modulation with adaptive optics: a novel approach for speckle reduction and structure extraction in optical coherence tomography

Zhang

Manna

Miller

et al. 2019

Biomed. Opt. Express

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Speckle is an inevitable consequence of the use of coherent light in imaging and acts as noise that corrupts image formation in most applications. Optical coherence tomographic imaging, as a technique employing coherence time gating, suffers from speckle. We present here a novel method of suppressing speckle noise intrinsically compatible with adaptive optics (AO) for confocal coherent imaging: modulation of the phase in the system pupil aperture with a segmented deformable mirror (DM) to introduce minor perturbations in the point spread function. This approach creates uncorrelated speckle patterns in a series of images, enabling averaging to suppress speckle noise while maintaining structural detail. A method is presented that efficiently determines the optimal range of modulation of DM segments relative to their AO-optimized position so that speckle noise is reduced while image resolution and signal strength are preserved. The method is active and independent of sample properties. Its effectiveness and efficiency are quantified and demonstrated by both ex vivo non-biological and in vivo biological applications.

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Section: Future Directionsmentioning

confidence: 99%

Aperture phase modulation with adaptive optics: a novel approach for speckle reduction and structure extraction in optical coherence tomography

Zhang

Manna

Miller

et al. 2019

Biomed. Opt. Express

View full text Add to dashboard Cite

show abstract

“…The marriage of modern AO and ultrahigh speed OCT scan technologies should enable routine implementation of APM-AO-OCT in clinical and research settings, enhancing cellular resolution clinical diagnosis as well as basic science discovery. For example, implementation of APM-AO-OCT should lead to broader application of OCT to brain structural imaging 60,61 where the presence of speckle in images remains a major obstacle. Finally, thanks to the improvement in the visualization of subcellular structure, APM-AO-OCT should enable a more precise localization and quantification of retinal optophysiological signals, which provide non-invasive, label-free measurement of photoreceptor function [62][63][64]…”

Section: Future Directionsmentioning

confidence: 99%

Aperture Phase Modulation with Adaptive Optics: A Novel Approach for Speckle Reduction and Structure Extraction in Optical Coherence Tomography

Zhang

Manna

Miller

et al. 2018

Preprint

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Speckle is an inevitable consequence of the use of coherent light in optical coherence tomography (OCT), and often acts as noise that obscures micro-structures of biological tissue. We here present a novel method of suppressing speckle noise intrinsically compatible with adaptive optics (AO) in OCT system: by modulating the phase inside the imaging system pupil aperture with a segmented deformable mirror, thus producing minor perturbations in the point spread function (PSF) to create un-correlated speckle pattern between B-scans, and further averaging to wash out the speckle but maintain the structures. It is a well-controlled and universal method which can efficiently determine the optimal range of phase modulation that minimizing speckle noise while maximizing image resolution and signal strength for different systems and/or samples. As an active method, its effectiveness and efficiency were demonstrated by both ex-vivo non-biological and in-vivo biological applications. Introduction:Speckle is a long-standing issue in all imaging technologies that use coherent light sources 1-4 , arised from the interference between light scattered by a random distributed scatters inside the system point-spread function (PSF), and observed as voxel-to-voxel intensity fluctuations in the image 5, 6 . Although speckle is a potential useful information about the dynamics of sample microstructure, in most applications it acts as a major noise source that degrades image quality. Optical coherence tomography (OCT) is a volumetric imaging technology developed in 1991 7 . It has been soon adopted in many biomedical applications 8-12 . However, as a method dependent on the coherent properties of light, OCT images suffer from speckle noise [13][14][15] .Many approaches have been taken to suppress speckle, including generation of multiple images by various means with uncorrelated speckle patterns, followed by averaging [16][17][18][19] . A weakness of these methods is that the number of uncorrelated speckle patterns that can be created is typically small, limiting speckle suppression by averaging. Speckle reduction methods using digital post-processing have also been proposed 20-23 . However, digital post-processing usually reduces speckle by spatial averaging or filtering, which necessarily reduces image resolution. Recently, it was shown that simple averaging of suitably numerous, well aligned images can reduce speckle for in vivo imaging, and it was hypothesized that subcellular motility Fig. 1 | System setup (photo in SI: Fig. S2) and the geometry of the segmented deformable mirror. a, OCT sample arm (Inset: (i) -Photo of the DM; (ii) -setup for USAF 1951 resolution test target; (iii)covariance analysis of the random segments pistons). b, Mirror configuration -Flat. c, Mirror Configuration -mirror segments with random displacements [SI: Video_S1]. d, Histogram of the mirror displacements for 100 mirror configurations in which the displacement range was 1m (0  0.5 m); Abbreviations: L#: Lens, VL: variable focus length liquid lens, ...

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Intraoperative Imaging Modalities and the Potential Role of Speckle Modulating Optical Coherence Tomography

2018

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High-resolution wide-field human brain tumor margin detection and in vivo murine neuroimaging

Cited by 3 publications

References 55 publications

Aperture phase modulation with adaptive optics: a novel approach for speckle reduction and structure extraction in optical coherence tomography

Aperture phase modulation with adaptive optics: a novel approach for speckle reduction and structure extraction in optical coherence tomography

Aperture Phase Modulation with Adaptive Optics: A Novel Approach for Speckle Reduction and Structure Extraction in Optical Coherence Tomography

Intraoperative Imaging Modalities and the Potential Role of Speckle Modulating Optical Coherence Tomography

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