Original scientific paper Slaughterhouse waste can be very dangerous and potential risk for animal and human health. Brains and spinal cords are deemed high-risk substances and can be infected with prions; their treatment is therefore only possible in strictly controlled conditions. One of the methods which can achieve the necessary reduction in health risk is alkaline hydrolysis. Standard alkaline hydrolysis is at 150 °C, 3-6 hours, and 4 bars. In this investigation focus was on different alkaline conditions: temperature (135 °C, 150 °C, 153 °C), time (2, 3, 6 hours), and base (NaOH and KOH) due to the fact that amino acids are sensitive to variations on temperature, pressure and base media. Analyzed amino acid composition of hydrolyzed material at different testing conditions show successful hydrolysis. The highest value of protein, which is generally relatively low, was achieved with NaOH at a temperature of 135 °C and time of 2 hours, whereas in the reaction with KOH the highest obtained value was achieved at 150 °C and 3 hours. The dry substance was increasing with time, and the highest value was achieved at the temperature of 150 °C and time 6 hours for both bases (NaOH, and KOH). Most of the amino acids went full racemization during the process; especially in D-amino acids like aspartic acid, glutamic acid, tryptophan and isoleucine. Lysine shows higher resistance to alkaline medium than other amino acids. Isolation of certain amino acids, like: asparagine, glutamic acid, glycine, alanine, and leucine, is possible because they show higher concentrations in hydrolysates.
Keywords: alkaline hydrolysis parameters; health risks; slaughterhouse waste; waste management
Obrada visokorizičnog bio otpada metodom alkalne hidrolize i izolacija amino kiselinaIzvorni znanstveni članak Nusproizvod životinjskog podrijetla vrlo je opasan za životinje i ljudsko zdravlje. Mozak životinja i leđna moždina su vrlo rizični te mogu biti zaraženi prionima te je stoga vrlo važno da se zbrinjavanje ovakvog materijala obavlja u strogo kontroliranim uvjetima. Metoda kojom se postiže smanjenje rizika za zdravlje ljudi je metoda alkalne hidrolize. Standardna metoda alkalne hidrolize odvija se pri 150 °C, 3 h do 6 h i tlaku od 4 bara. Rad donosi istraživanje na polju izolacije amino kiselina nakon provedene alkalne hidrolize pri različitim uvjetima temperature (135 °C, 150 °C, 153 °C), vremena (2 h, 3 h, 6 h) i lužinama (NaOHi KOH) uzimajući u obzir činjenicu da su amino kiseline osjetljive na promjenu temperature, lužine i tlaka. Analizirani hidrolizat pokazuje uspješno provedenu alkalnu hidrolizu pri različitim eksperimentalnim uvjetima.Veći sadržaj proteina, koji je generalno u svim uzorcima relativno nizak, postignut je s NaOH pri temperature 135 °C i vremenu 2 h, dok je u reakciji s KOH veća koncentracija proteina postignuta pri 150 °C i 3 h. Suha tvar se povećavala s duljinom hidroliziranja te je najveća vrijednost postignuta pri temperaturi od 150 °C i vremenu od 6 h s obje lužine. Većina aminokiselina je prošla process racemiza...