High risk HPV types 18 and 16 are potent modulators of oral squamous cell carcinoma phenotypes in vitro

Reddout, Nicole; Christensen, Todd; Bunnell, Anthony; Jensen, Dayne; Johnson, Devin; O’Malley, Susan; Kingsley, Karl

doi:10.1186/1750-9378-2-21

Cited by 36 publications

(35 citation statements)

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“…HPV16 has been considered as the predominant HPV subtype involved in oropharyngeal cancer [9], [10], [12]–[16], [27]. However, the infection rates for HPV18 were the same as for HPV16 (30.3%) in this study population (Table 1).…”

Section: Discussionmentioning

confidence: 71%

Differential Impact of IL-10 Expression on Survival and Relapse between HPV16-Positive and -Negative Oral Squamous Cell Carcinomas

et al. 2012

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Human papillomavirus (HPV) is a risk factor in a subset of oropharyngeal cancer; however, the contribution of HPV in the malignancy of oral squamous cell carcinomas (OSCC) is not fully understood in Taiwanese. Herein, 61 patients with no risk factors and 117 patients with one or more risk factors were enrolled in this study. HPV16/18 infection rate in non-smokers, non-drinkers and non-betel quid chewers was higher than their counterparts. The development of HPV-infected cancer has been shown to be associated with interleukin-10 (IL-10) expression. To this end, IL-10 mRNA expression in OSCC tumors was evaluated by real-time RT-PCR. Data showed that HPV-positive patients had higher IL-10 mRNA levels than in HPV-negative patients. Kaplan-Meier and Cox-regression analysis indicated that the prognostic significance of IL-10 mRNA on overall survival and relapse free survival was only observed in HPV-positive OSCC, but not in HPV-negative OSCC. Mechanistically, the elevation of IL-10 by E6 was responsible for increased colony formation and migration capability in OSCC cells. Therefore, we suggest that IL-10 induced by E6 promotes cell growth and migration capability and consequent poor survival and relapse in HPV-positive OSCC.

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Section: Discussionmentioning

confidence: 71%

Differential Impact of IL-10 Expression on Survival and Relapse between HPV16-Positive and -Negative Oral Squamous Cell Carcinomas

et al. 2012

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“…Cell number was determined using Trypan Blue (Fisher Scientific: Fair Lawn, New Jersey, USA) using a Zeiss Axiovert 40 inverted microscope (Carl Ziess, Inc: Thornwood, New York, USA) and a hemacytometer (Fisher Scientific: Fair Lawn, New Jersey, USA). To determine if any samples harbored the HPV virus, DNA was isolated from the saliva sample using a minimum of 3.5 x 10 5 cells using the GenomicPrep DNA isolation kit (Amersham Biosciences: Buckinghamshire, United Kingdom), using the procedure recommended by the manufacturer as previously described [12,42,78,79]. DNA purity was calculated using ratio measurements of absorbance at 260 and 280 nm (A260/A280 ratio between 1.7 and 2.0).…”

Section: Methodsmentioning

confidence: 99%

“…DNA from each sample was then used to perform PCR with the Fisher exACTGene complete PCR kit (Fisher Scientific: Fair Lawn, New Jersey, USA) and a Mastercycler gradient thermocycler (Eppendorf: Hamburg, Germany) using the following primers for HPV16 [12,78], HPV18 [12,78,79], and glyceraldehyde- 3- phosphate dehydrogenase (GAPDH) [80] (SeqWright: Houston, Texas, USA):…”

Section: Methodsmentioning

confidence: 99%

Screening and detection of human papillomavirus (HPV) high-risk strains HPV16 and HPV18 in saliva samples from subjects under 18 years old in Nevada: a pilot study

et al. 2012

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BackgroundHuman papillomaviruses (HPV) are oncogenic and mainly associated with cervical cancers. Recent evidence has demonstrated HPV infection in other tissues, including oral epithelia and mucosa. Although a recent pilot study provided new information about oral HPV status in healthy adults from Nevada, no information was obtained about oral HPV prevalence among children or teenagers, therefore, the goal of this study is to provide more detailed information about oral prevalence of high-risk HPV among children and teenagers in Nevada.MethodsThis retrospective study utilized previously collected saliva samples, obtained from pediatric dental clinic patients (aged 2 – 11) and local school district teenagers (aged 12-17) for high-risk HPV screening (n=118) using qPCR for quantification and confirmation of analytical sensitivity and specificity.ResultsA small subset of saliva samples were found to harbor high-risk HPV16 (n=2) and HPV18 (n=1), representing a 2.5% of the total. All three were obtained from teenage males, and two of these three samples were from White participants.ConclusionsAlthough this retrospective study could not provide correlations with behavioral or socioeconomic data, this project successfully screened more than one hundred saliva samples for high-risk HPV, confirming both HPV16 and HPV18 strains were present in a small subset. With increasing evidence of oral HPV infection in children, this study provides critical information of significant value to other dental, medical, oral and public health professionals who seek to further an understanding of oral health and disease risk in pediatric populations.

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“…The results have shown that HPV could be involved in the onset of certain types of carcinoma. In the last few years, the literature has provided evidence that HPV can be an independent risk factor for squamous cell carcinoma (9,10). Case-control studies such as the one performed in 2008 by Pintos J et al (9), show that infection from highrisk HPV is associated with 19% of cases and 5% of controls.…”

Section: Resultsmentioning

confidence: 99%

Papilloma virus and oral lesions. Infection and increased risk of potential malignant transformation. A systematic review

Palaia¹

2014

PER

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Background: oral cancer has been associated with several risk factor, such as smoking, alcohol, irritants and irradiation. The presence of HPV in oral cancers suggests that HPV may play a similar role in transforming oral epithelia. Objectives: a significant association was appreciated between infection with human papillomavirus (HPV), squamous cell carcinoma and potentially malignant disorders of the oral cavity, such as leukoplakia, erythro-leukoplakia erythroplakia. This review will attempt to focus on relevant characteristics of HPV, analyze its role in oral cancer and discuss some emerging developments. Methods: from the critical analysis of the current literature, many studies evaluating different markers of exposure and viral activity in tumors were collected. Several studies have investigated the prevalence of HPV in these cancers, but the prevalence of HPV detection varies broadly, depending on the population, combination of sub-sites, typology of specimen and method of detection. Results: the majority of HPV-related cancers contain HPV DNA integrated into the host cell genome and express only two viral genes, E6 and E7, both of which encode oncoproteins. Data published in the literature have provided strong evidence that HPVs may be the cause of a defined subset of head and neck cancers and also an indicator of improved survival. Discussion: while the role of HPV infection in the onset of cervix cancer is now well established, there is little information on the prevalence, determinants and natural history of the infection in the oral mucosa, and further studies are needed to clarify the potential role of HPV in the onset of oral cancer.

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High risk HPV types 18 and 16 are potent modulators of oral squamous cell carcinoma phenotypes in vitro

Cited by 36 publications

References 35 publications

Differential Impact of IL-10 Expression on Survival and Relapse between HPV16-Positive and -Negative Oral Squamous Cell Carcinomas

Differential Impact of IL-10 Expression on Survival and Relapse between HPV16-Positive and -Negative Oral Squamous Cell Carcinomas

Screening and detection of human papillomavirus (HPV) high-risk strains HPV16 and HPV18 in saliva samples from subjects under 18 years old in Nevada: a pilot study

Papilloma virus and oral lesions. Infection and increased risk of potential malignant transformation. A systematic review

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