A doppler delay-time frequency (DD-TF) crossdomain high-security transmission scheme based on orthogonal time frequency space (OTFS) is proposed, which is compatible with high-speed and large-capacity optical transmission systems. In this scheme, 36 sets of chaotic sequences are mapped out through a two-dimensional chaotic system with variable control parameters and variable initial values for optical physical layer encryption. The data modulated based on OTFS is encrypted by multiple sets of chaotic sequences, achieving cross-domain joint encryption in the DD domains and TF domains. This method improves security performance of the optical transmission system. An experiment is conducted, in which the encrypted 16quadrature amplitude modulation (QAM) OTFS signals over 25km standard single-mode fiber (SSMF) is achieved, and the key space is 10 1140 to effectively prevent malicious attacks from illegal Optical Network Units (ONUs). This key space is the reported maximum key space of the physical layer of two-dimensional chaotic systems. Compared with orthogonal frequency division multiplexing (OFDM) encryption scheme, this scheme obtains better transmission performance. It is shown that OTFS system with cyclic prefix (CP) of 1/16 obtains 2.1dB gain than the OTFS system with CP of 1/32 in terms of receiver sensitivity when the bit error rate (BER) is 10 -3 . Due to its comprehensive advantages in error rate and security performance, this chaotic encryption based on OTFS has a high application prospect in low-cost and reliable optical access system.