2022
DOI: 10.1021/acs.analchem.2c03921
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High-Sensitivity Amperometric Dual Immunoassay Using Two Cascade Reactions with Signal Amplification of Redox Cycling in Nanoscale Gap

Abstract: Here, we report a high-sensitivity dual immunoassay using Lumulus amebocyte lysate (LAL) and blood coagulation cascade reactions with redox cycling in a nanoscale-gap electrode. Endotoxin and factor XIa were used as the label molecules for the immunoassay of two types of analytes to induce the LAL and coagulation cascade reactions, respectively, when each corresponding analyte existed in the sample solution. In addition to the signal amplification by the cascade reactions, we employed redox cycling in a nanosc… Show more

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Cited by 3 publications
(4 citation statements)
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“…In the future, further characterization of the Au flat plate as a quasi-reference electrode may result in a highly portable device. The IDAE device has a robust structure compared to the redox cycling device in a nanoscale gap [ 19 , 22 , 23 ]. The redox cycling device in a nanoscale gap can break as a result of a small impact due to the broken spatial structure, which is not the case with the IDAE device.…”
Section: Resultsmentioning
confidence: 99%
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“…In the future, further characterization of the Au flat plate as a quasi-reference electrode may result in a highly portable device. The IDAE device has a robust structure compared to the redox cycling device in a nanoscale gap [ 19 , 22 , 23 ]. The redox cycling device in a nanoscale gap can break as a result of a small impact due to the broken spatial structure, which is not the case with the IDAE device.…”
Section: Resultsmentioning
confidence: 99%
“…Additionally, an IDAE device with a 10 μm electrode distance is easier to produce than a device for redox cycling in a nanoscale gap because devices with a small electrode distance are difficult to manufacture, which is an important factor when considering mass production. In our previous study involving a device for redox cycling in a nanoscale gap [ 19 , 22 , 23 ], the shape of amperograms was influenced by the adsorption and desorption of a redox molecule to the electrode surface [ 30 ]. A LAL reagent without a chromogenic substrate can be effectively used to obtain 1.0 EU/L of the limit of detection with 30 min of the LAL reaction by using the IDAE device.…”
Section: Resultsmentioning
confidence: 99%
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“…In this process, a small number of signal molecules can produce a significantly enhanced electrochemical signal. Nowadays, electrochemical immunosensors based on the signal amplification of enzymes plus electrochemical–chemical (EC) or electrochemical–chemical–chemical (ECC) redox cycling arouse wide interest due to their excellent sensitivity and selectivity [ 112 , 113 , 114 , 115 , 116 , 117 ].…”
Section: Electrochemical Methodsmentioning
confidence: 99%