High-sensitivity detection of two H7 subtypes of avian influenza viruses (AIVs) by immunochromatographic assay with highly chromatic red silica nanoparticles

Abstract

In this work, high chromaticity red SiNPs were for the first time used as novel visual markers in ICA for the detection of H7 subtype influenza viruses, and the quantitative detection of the avian influenza H7 HA virus (H7N2 and H7N9) was realized by sandwich immunoassay.

Progress in Treatment and Diagnostics of Infectious Disease with Polymers

“…These probes were used to capture the two H7 subtypes. 73 Therapeutics and Vaccination. Supermagnetic Fe 3 O 4 NPs coated with PVP and PEG show improved biocompatibility, less toxicity and more H1N1 virus inhibition in vitro.…”

“…The electrostatic self-assembly was designed by electrostatic interaction of red silica NPs with cationic polyethylenimine (PEI) coupled to antibody. These probes were used to capture the two H7 subtypes …”

A nanoparticle-assisted signal-enhancement technique for lateral flow immunoassays

“…In the fluorometric mode, this method demonstrated a substantial enhancement in sensitivity for detecting the monkeypox virus, showing 238-fold and 3.3-fold improvements compared to the colloidal Au-based LFIA and ELISA methods, respectively. 46 Dou et al prepared the dye-doped silica nanobeads with intensified fluorescence intensity through the PEI and poly(sodium- p -styrenesulfonate)-mediated layer-by-layer self-assembly approach, 103 The fluorescence signal of the resultant nanobeads (dyeing four times) was significantly enhanced compared to the once-dyed silica nanoparticles. 103 The dye-doped silica nanobead-based LFIA realised the quantitative detection of hemagglutinin antigen of H7N2 and H7N9 avian influenza viruses (AIVs), in the linear range of 0.1–10 ng mL −1 and LoD of 0.08 pg mL −1 .…”

“…46 Dou et al prepared the dye-doped silica nanobeads with intensified fluorescence intensity through the PEI and poly(sodium-p-styrenesulfonate)-mediated layer-by-layer self-assembly approach, 103 The fluorescence signal of the resultant nanobeads (dyeing four times) was significantly enhanced compared to the once-dyed silica nanoparticles. 103 The dyedoped silica nanobead-based LFIA realised the quantitative detection of hemagglutinin antigen of H7N2 and H7N9 avian influenza viruses (AIVs), in the linear range of 0.1-10 ng mL À1 and LoD of 0.08 pg mL À1 . 103 The noble metal nanomaterials, such as Au, [105][106][107] and Ag, 108 can be formed in situ on the surface of silica nanoparticles.…”

“…103 The dyedoped silica nanobead-based LFIA realised the quantitative detection of hemagglutinin antigen of H7N2 and H7N9 avian influenza viruses (AIVs), in the linear range of 0.1-10 ng mL À1 and LoD of 0.08 pg mL À1 . 103 The noble metal nanomaterials, such as Au, [105][106][107] and Ag, 108 can be formed in situ on the surface of silica nanoparticles. Typically, the process begins with mixing the silica nanoparticles with solutions containing seeds [105][106][107] or ions 108 to form the seed/ion-decorated silica nanoparticles.…”