High Sensitivity Immunoassays Using Particulate Fluorescent Labels

Upconverting Phosphor Reporters in Immunochromatographic Assays

“…The 96-well plate assay format was adapted from a protocol we had developed for fluorescent microspheres (15). Briefly, Nunc Maxisorb 96-well plates were coated with 1 g sheep anti-hCG plus 25 g BSA/well for 24 h at 4°C.…”

Wash-LOCI - A Semi-Heterogeneous Version of the LOCI Technology Allowing Removal of Unbound Material After Each Assay Step

“…A separation could be done by binding antibody-A beads to the well bottom, by a filtration step or by using magnetic beads (Hall et al, 1999;Koskinen et al, 2004;Kulmala et al, 2002;McCrindle et al, 1985;Namba et al, 2000;Obenauer-Kutner et al, 1997;Okano et al, 1992;Sardesai et al, 2009;Sin et al, 2006;Soukka et al, 2001). All the described methods are based on particulate labels.…”

Double labeling and comparison of fluorescence intensity and photostability between quantum dots and FITC in oral tumors

“…Functionalized bio-affinity nano fluorescence probes have recently been developed based on semiconductor quantum dots (Qds), and have very broad prospective uses in biomedical applications, particularly in the study of bio-imaging in oncology, due to their unique optical properties. Qds have already been applied to the analysis of membrane proteins in mammary cancer and have been used as a research tool for the molecular imaging of blood cells (3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14). However, there have been no reports on the application of Qds in the study of proteins in human tongue cancer cells.…”