High Sensitivity Luminescence Nanoparticle Assay for the Detection of Protein Aggregation

Pihlasalo, Sari; Kirjavainen, Jonna; Hänninen, Pekka; Härmä, Harri

doi:10.1021/ac1026385

Cited by 30 publications

(20 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Nanoparticulate approach has attracted increasing interest in recent years1319202122. Novel MRI CA based on gadolinium-containing nanoparticles show enhanced relaxation parameters compared to commercial specimens1323242526.…”

mentioning

confidence: 99%

High performance magneto-fluorescent nanoparticles assembled from terbium and gadolinium 1,3-diketones

Zairov

Mustafina

Shamsutdinova

et al. 2017

Sci Rep

View full text Add to dashboard Cite

Polyelectrolyte-coated nanoparticles consisting of terbium and gadolinium complexes with calix[4]arene tetra-diketone ligand were first synthesized. The antenna effect of the ligand on Tb(III) green luminescence and the presence of water molecules in the coordination sphere of Gd(III) bring strong luminescent and magnetic performance to the core-shell nanoparticles. The size and the core-shell morphology of the colloids were studied using transmission electron microscopy and dynamic light scattering. The correlation between photophysical and magnetic properties of the nanoparticles and their core composition was highlighted. The core composition was optimized for the longitudinal relaxivity to be greater than that of the commercial magnetic resonance imaging (MRI) contrast agents together with high level of Tb(III)-centered luminescence. The tuning of both magnetic and luminescent output of nanoparticles is obtained via the simple variation of lanthanide chelates concentrations in the initial synthetic solution. The exposure of the pheochromocytoma 12 (PC 12) tumor cells and periphery human blood lymphocytes to nanoparticles results in negligible effect on cell viability, decreased platelet aggregation and bright coloring, indicating the nanoparticles as promising candidates for dual magneto-fluorescent bioimaging.

show abstract

mentioning

confidence: 99%

High performance magneto-fluorescent nanoparticles assembled from terbium and gadolinium 1,3-diketones

Zairov

Mustafina

Shamsutdinova

et al. 2017

Sci Rep

View full text Add to dashboard Cite

show abstract

“…3E). Since the accumulation of unfolded proteins in mitochondria has been shown to induce mitophagy in neurons 46 , we next investigated the presence of unfolded proteins using a colorimetric assay that has been used to identify amyloid and other unfolded proteins in experimental disease processes 47–51 . At an early time point, Brg1/Brm double-mutant hearts did not have an increase in unfolded proteins (Fig.…”

Section: Resultsmentioning

confidence: 99%

BRG1 and BRM SWI/SNF ATPases redundantly maintain cardiomyocyte homeostasis by regulating cardiomyocyte mitophagy and mitochondrial dynamics in vivo

Bultman

Holley

Ridder

et al. 2016

Cardiovascular Pathology

View full text Add to dashboard Cite

There has been an increasing recognition that mitochondrial perturbations play a central role in human heart failure. Discovery of mitochondrial networks, whose function is to maintain the regulation of mitochondrial biogenesis, autophagy (‘mitophagy’) and mitochondrial fusion/fission, are new potential therapeutic targets. Yet our understanding of how the molecular underpinning of these processes is just emerging. We recently identified a role of the SWI/SNF ATP-dependent chromatin remodeling complexes in the metabolic homeostasis of the adult cardiomyocyte using cardiomyocyte-specific and inducible deletion of the SWI/SNF ATPases BRG1 and BRM in adult mice (Brg1/Brm double mutant mice). To build upon these observations in early alterated metabolism, the present study looks at the subsequent alterations in mitochondrial quality control mechanisms in the impaired adult cardiomyocyte. We identified that Brg1/Brm double-mutant mice exhibited an increased mitochondrial biogenesis, increases in ‘mitophagy’, and alterations in mitochondrial fission and fusion that led to small, fragmented mitochondria. Mechanistically, increases in the autophagy and mitophagy-regulated proteins Beclin1 and Bnip3 were identified, paralleling changes seen in human heart failure. Cardiac mitochondrial dynamics were perturbed including decreased mitochondria size, reduced number, and altered expression of genes regulating fusion (Mfn1, Opa1) and fission (Drp1). We also identified cardiac protein amyloid accumulation (aggregated fibrils) during disease progression along with an increase in pre-amyloid oligomers and an upregulated unfolded protein response including increased GRP78, CHOP, and IRE-1 signaling. Together, these findings described a role for BRG1 and BRM in mitochondrial quality control, by regulating mitochondrial number, mitophagy, and mitochondrial dynamics not previously recognized in the adult cardiomyocyte. As epigenetic mechanisms are critical to the pathogenesis of heart failure, these novel pathways identified indicate that SWI/SNF chromatin remodeling functions are closely linked to mitochondrial quality control mechanisms.

show abstract

“…Some researches about PL sensitivity to surrounding changes have been reported before [ 24 , 25 ]. For example, as reported in a previous paper, the authors investigated nanosecond photoluminescence processes in colloidal core/shell CdSe/ZnS nanoparticles dissolved in water and found strong sensitivity of luminescence to the solvent state.…”

Section: Resultsmentioning

confidence: 99%

Water-Soluble Silicon Quantum Dots with Quasi-Blue Emission

Wang

Guo

et al. 2015

Nanoscale Res Lett

View full text Add to dashboard Cite

In this study, water-soluble silicon quantum dots have quasi-blue emission at 390 nm by being capped with 1-vinylimidazole in resese micelles. As-obtained silicon quantum dots have a diameter of 2~5 nm and high crystallinity. The quasi-blue emission of the silicon quantum dots is likely attributed to the polarity of the capping ligands. Moreover, the silicon quantum dots are water-soluble and have photoluminescence nanosecond decay time, suggesting their potential application in biological field.

show abstract

High Sensitivity Luminescence Nanoparticle Assay for the Detection of Protein Aggregation

Cited by 30 publications

References 22 publications

High performance magneto-fluorescent nanoparticles assembled from terbium and gadolinium 1,3-diketones

High performance magneto-fluorescent nanoparticles assembled from terbium and gadolinium 1,3-diketones

BRG1 and BRM SWI/SNF ATPases redundantly maintain cardiomyocyte homeostasis by regulating cardiomyocyte mitophagy and mitochondrial dynamics in vivo

Water-Soluble Silicon Quantum Dots with Quasi-Blue Emission

Contact Info

Product

Resources

About