In this paper, a novel metamaterial sensor with excellent sensitivity and quality factor for microwave sensing applications is presented. The designed metamaterial sensor is assembled on a 1.575 mm thickness of low-cost dielectric substrate material (Rogers RT5880), and the copper is used as a resonator. Computer Simulation Technology version 2019 (CST-2019) software is employed to design and analyze the proposed metamaterial sensor. In addition, the Advanced Design System version 2016 (ADS 2016) software is used to validate the CST simulated model. Subsequently, the simulated results were validated using laboratory measurements. The optimized cell is small; its dimension is 10 × 10 mm2, and the obtained resonances are 3.85 and 6.85 GHz with notches of −26.29 and −40.03 dB, respectively. The textile material is detected by the resonance frequency change, and this frequency is dependent on the material’s permittivity values. To test the developed sensor’s sensing capabilities, three types of textiles—wool, fleece, and denim—are used. The effective medium ratio, sensitivity, and Q-factor of the structure are evaluated, and the obtained values are 8.96, 14.57%, and 345, respectively. The sensor for detecting textile materials works in the S and C bands. The resonances are shifted 530 MHz between the air and wool, 420 MHz between the air and fleece, and 640 MHz between the air and denim. The simulated outcomes and laboratory results almost matched. The projected sensor can be employed in the apparel sector to identify textile materials because it is small, inexpensive, has a high quality factor, and has high sensitivity.