High sensitivity point-of-care device for direct virus diagnostics

Kiilerich-Pedersen, Katrine; Daprà, Johannes; Cherré, Solène; Rozlosnik, Noémi

doi:10.1016/j.bios.2013.05.046

Cited by 66 publications

(42 citation statements)

References 32 publications

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“…Recently, a whole range of virus-specific aptamers were generated against vaccinia virus [36,40], dengue virus [41], severe acute respiratory syndrome (SARS) [42][43][44][45], hepatitis C [46][47][48][49][50][51], human immunodeficiency virus (HIV) [52][53][54][55], apple stem pitting virus [56][57][58], bovine viral diarrhea virus [39], norovirus [34,59], rabies virus [60], hepatitis B [61], Ebola [62] and influenza [31,33,35,[63][64][65][66][67][68][69][70][71][72][73][74].…”

Section: Application Of Aptamers For Virus Detectionmentioning

confidence: 99%

Aptasensors for viral diagnostics

Kieboom

Beek

Mészáros

et al. 2015

TrAC Trends in Analytical Chemistry

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Novel viral diagnostic tools need to be affordable, fast, accurate and easy to use with sensitivity and specificity equivalent or superior to current standards. At present, viral diagnostics are based on direct detection of viral components or indirect detection by measuring antibodies generated in response to viral infection. While sensitivity of detection and quantification are still important challenges, we expect major advances from new assay formats and synthetic binding molecules, such as aptamers. Compared to traditional antibody-based detection, aptamers could provide faster adaptation to continuously evolving virus strains and higher discriminating capacity between specific virus serotypes. Aptamers are very stable and easily modifiable, so are ideal molecules for detection and chemical sensing applications. Here, we review the use of aptasensors for detection of viral pathogens and consider the feasibility of aptasensors to become standard devices for point-of-care diagnostics of viruses.

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Section: Application Of Aptamers For Virus Detectionmentioning

confidence: 99%

Aptasensors for viral diagnostics

Kieboom

Beek

Mészáros

et al. 2015

TrAC Trends in Analytical Chemistry

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“…149 Many EIS detection systems for various viruses have been described, but most of them were dedicated to influenza detection, based on utilization of anti-influenza antibody or aptamers. Kiilerich-Pedersen et al 150 reported a study where influenza A (H1N1) virus aptamers were linked covalently to the conductive polymer microelectrodes. H1N1 virions were captured by immobilized probes, and detected as changes in the impedance.…”

Section: Eis Immunobiosensormentioning

confidence: 99%

Nanoscale virus biosensors: state of the art

Krejčová¹,

Michálek²,

Rodrigo³

et al. 2015

NDD

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Abstract:Since the beginning of the new millennium, viruses have shown huge epidemiological and pandemic potential: severe acute respiratory syndrome (SARS) in 2002, pandemic swine flu in 2009, and last but not least the West African Ebola outbreak in 2014. The occurrence and spread of the new virus in pandemic dimension poses a threat to the health and lives of 7 billion people worldwide. There is a growing urgency for highly sensitive and selective detection techniques, usable for a wide number of applications, including disease diagnosis, pharmaceutical research, agriculture, as well as preventive measures. Nanobiosensors represent a new promising tool for virus detection. This review gives a brief survey of the issue of viral detection, comprising diagnostics of target structure of viruses such as nucleic acids or proteins. This review covers different detection principles, methods of fabrication, and applications of virus biosensors.

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“…Poor detection sensitivity, being time-consuming and risky are important factors to take into account when choosing the best assay in clinical and public health laboratories [19, 20]. Moreover, the constant mutational changes of the viral genome during the evolutionary process may pose a major challenge in developing a universal method or system with the ability to detect different types and strains of the viruses [21].…”

Section: Introductionmentioning

confidence: 99%

A diagnostic one-step real-time reverse transcription polymerase chain reaction method for accurate detection of influenza virus type A

Behzadi¹,

Ziyaeyan²,

Alborzi³

2016

aoms

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IntroductionInfluenza A is known as a public health concern worldwide. In this study, a novel one-step real-time reverse transcription polymerase chain reaction (rtRT-PCR) assay was designed and optimized for the detection of influenza A viruses.Material and methodsThe primers and probe were designed based on the analysis of 90 matrix nucleotide sequence data of influenza type A subtypes from the GenBank database of the National Center for Biotechnology Information (NCBI). The influenza virus A/Tehran/5652/2010 (H1N1 pdm09) was used as a reference. The rtRT-PCR assay was optimized, compared with that of the World Health Organization (WHO), and its analytical sensitivity, specificity and reproducibility were evaluated. In total, 64 nasopharyngeal swabs from patients with influenza-like illness (ILI) and 41 samples without ILI symptoms were tested for the virus, using conventional cell culture, direct immunofluorescence antibody (DFA) methods, and one-step rtRT-PCR with the designed primer set and probe and the WHO’s.ResultsThe optimized assay results were similar to the WHO’s. The optimized assay results were similar to WHO’s, with non-significant differences for 10–103 copies of viral RNA/reaction (p > 0.05). It detected 10 copies of viral RNA/reaction with high reproducibility and no cross reactivity with other respiratory viruses. A specific cytopathic effect was observed in 6/64 (9.37%) of the ILI group using conventional culture and DFA staining methods; however, it was not seen in non-ILI. Also, the results of our assay and the WHO’s were similar to those of viral isolation and DFA staining.ConclusionsGiven the high specificity, sensitivity and reproducibility of this novel assay, it can serve as a reliable diagnostic tool for the detection of influenza A viruses in clinical specimens and lab experiments.

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High sensitivity point-of-care device for direct virus diagnostics

Cited by 66 publications

References 32 publications

Aptasensors for viral diagnostics

Aptasensors for viral diagnostics

Nanoscale virus biosensors: state of the art

A diagnostic one-step real-time reverse transcription polymerase chain reaction method for accurate detection of influenza virus type A

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