High sensitivity simultaneous determination in hair of the major constituents of ecstasy (3,4-methylenedioxymethamphetamine, 3,4-methylenedioxyamphetamine and 3,4-methylenedioxyethylamphetamine) by high-performance liquid chromatography with direct fluorescence detection

Tagliaro, Franco; Battisti, Z. De; Groppi, Angelo; Nakahara, Yoshiaki; Scarcella, D.; Valentini, R; Marigo, Mario

doi:10.1016/s0378-4347(98)00508-8

Cited by 30 publications

(15 citation statements)

References 24 publications

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“…For plasma different LC-MS methods for the determination of amphetamines and designer drugs have been published [24,27,[29][30][31][32]. Despite these modern techniques, traditionally the most widely used technique for the determination of designer drugs by high performance liquid chromatography (HPLC), was HPLC with fluorescence detection [33][34][35][36][37][38][39][40].…”

Section: Introductionmentioning

confidence: 99%

Fast LC–MS/MS method for the determination of amphetamine, methamphetamine, MDA, MDMA, MDEA, MBDB and PMA in urine

Concheiro

Simões

Quintela

et al. 2007

Forensic Science International

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Section: Introductionmentioning

confidence: 99%

Fast LC–MS/MS method for the determination of amphetamine, methamphetamine, MDA, MDMA, MDEA, MBDB and PMA in urine

Concheiro

Simões

Quintela

et al. 2007

Forensic Science International

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“…The escalation of the abuse of these drugs has caused serious health and social problems: chronic use of amphetamines often leads to hallucinations and psychosis, as well as dysphoria and depression upon withdrawal [1]. In response to a growing demand for reliable evidence of amphetamines use, methods providing reproducible results for identification and quantification of the amphetamines and related compounds in biological matrices have been developed using a variety of techniques such as HPLC [2,3], GC [4][5][6][7], and CE [8][9][10][11][12][13]. However, the trace amount of these substances in biological matrices including human hair remains a challenge for analytical chemists.…”

Section: Introductionmentioning

confidence: 99%

Analysis of amphetamine, methamphetamine and methylenedioxy‐methamphetamine by micellar capillary electrophoresis using cation‐selective exhaustive injection

et al. 2006

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Cation-selective exhaustive injection (CSEI) is used as an on-line concentration method for the high-sensitivity analysis of illicit amphetamines using CE. Optimum conditions for the determination of amphetamine, methamphetamine and methylenedioxy-methamphetamine were investigated. Sodium dodecyl sulfate (25 mM) in 100 mM phosphate buffer (pH 2.9) with 20% methanol as organic additive was used as the background electrolyte for CE separation. The LOD, based on an S/N of 3:1, was about 0.01 microg/mL using normal capillary micellar electrokinetic chromatography, while by using CSEI in combination with micellar sweeping the sensitivity increased up to 1000-fold with the LOD lower than 50 pg/mL. The reproducibility of CSEI combined with micellar sweeping for analyzing amphetamines was satisfactory (relative standard deviation around 10% by using area ratios against an internal standard). This method is highly sensitive and can be used to analyze trace amount amphetamines in human hair.

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“…These compounds, structurally similar to amphetamine (AP), have been known to enhance empathy and produce hallucination (Freese et al, 2002;Tagliaro et al, 1999). These compounds, structurally similar to amphetamine (AP), have been known to enhance empathy and produce hallucination (Freese et al, 2002;Tagliaro et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

Quantification of MDMA and MDA in abusers' hair samples by semi‐micro column HPLC with fluorescence detection

Nakamura

Tomita

Wada

et al. 2006

Biomedical Chromatography

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A sensitive semi-micro column high-performance liquid chromatography with fluorescence detection method was developed for the determination of 3,4-methylenedioxymethamphetamine (MDMA), 3,4-methylenedioxyamphetamine (MDA), methamphetamine (MP) and amphetamine (AP) in human hair. 4-(4,5-Diphenyl-1H-imidazol-2-yl)benzoyl chloride (DIB-Cl) and 1-methyl-3-phenylpropylamine were used as labeling reagent and internal standard, respectively. These drugs were extracted from hair into 5% trifluoroacetic acid in methanol, and fluorescent labeled with DIB-Cl. The separation of DIB-derivatives was achieved on a reversed-phase semi-micro ODS column with an acetonitrile-methanol-water (30:40:30, v/v/v%) mixture as a mobile phase. The limits of detection at a signal-to-noise ratio of 3 for MDMA, MDA, MP and AP were 0.25, 0.15, 0.25 and 0.19 ng/mg, respectively. Precision of intra- and inter-day assay as the relative standard deviation were in the range 1.5-6.8% (n = 5) and 2.7-4.7% (n = 5), respectively. The proposed method was highly sensitive and able to detect MDMA and its related compounds in small amounts of hair sample, and could be applied to quantification of six abusers' hair samples.

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High sensitivity simultaneous determination in hair of the major constituents of ecstasy (3,4-methylenedioxymethamphetamine, 3,4-methylenedioxyamphetamine and 3,4-methylenedioxyethylamphetamine) by high-performance liquid chromatography with direct fluorescence detection

Cited by 30 publications

References 24 publications

Fast LC–MS/MS method for the determination of amphetamine, methamphetamine, MDA, MDMA, MDEA, MBDB and PMA in urine

Fast LC–MS/MS method for the determination of amphetamine, methamphetamine, MDA, MDMA, MDEA, MBDB and PMA in urine

Analysis of amphetamine, methamphetamine and methylenedioxy‐methamphetamine by micellar capillary electrophoresis using cation‐selective exhaustive injection

Quantification of MDMA and MDA in abusers' hair samples by semi‐micro column HPLC with fluorescence detection

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