2024
DOI: 10.21203/rs.3.rs-4019952/v1
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High-sensitivity whole-mount in situ hybridization of mouse oocytes and embryos visualizes the super-resolution structures and distributions of mRNA molecules

Takahiro Sanada,
Tomoya Kotani

Abstract: Background Mammalian oocytes accumulate more than ten thousand mRNAs, of which three to four thousand mRNAs are translationally repressed. The timings and sites of translational activation of these dormant mRNAs are crucial for promoting oocyte maturation and embryonic development. How these mRNAs are accumulated and distributed in oocytes is therefore a fundamental issue to be explored. A method that enables visualization of mRNA molecules with high resolution in a simple manner would be valuable for underst… Show more

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