High-shore mussels, Mytilus californianus, have larger muscle fibers with lower aerobic capacities than low-shore conspecifics

Dowd, W. Wesley; Jiménez, Ana Gabriela

doi:10.1007/s00227-019-3471-y

Cited by 3 publications

(1 citation statement)

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“…This is consistent with metabolic properties of liver cell lines derived from cave and surface morphotypes from Astyanax mexicanus showing increased glycolytic metabolism in the cave morphs (Krishnan et al, 2022). In the ribbed mussel, Mytilus californianus, adductor muscle, CS activity was highest in low intertidal/higher O 2 environments compared with high intertidal/low O 2 environments (Dowd & Jimenez, 2019), similar to our findings here. Lactate dehydrogenase (LDH) and CS have also been previously negatively correlated in white muscle of other pelagic freshwater teleost species, Paralabrax clathratu and Salmo gairdneri , consistent with their relationship reflecting choice of metabolic strategy.…”

Section: Discussionsupporting

confidence: 91%

White epaxial muscle aerobic and anaerobic potential and muscle fiber structure in surface and cave morphotypes of the Mexican cavefish (Astyanax mexicanus)

2023

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Proper muscle function and muscle fiber structures that match the environmental demands of organisms are imperative to their success in any ecosystem. The Mexican cavefish, Astyanax mexicanus, has two morphotypes: an obligate cave‐dwelling form that lives in thermally insulated caves and an O2 poor environment, and a surface form that lives in a more thermally variable, but O2 rich river environment. As environment can determine physiological adaptations, it is of interest to compare the aerobic and anaerobic metabolic profiles of white muscle metabolism in both morphotypes of this species, as well as their muscle structures. Here, we used white muscle of both morphotypes of the Mexican cavefish to determine citrate synthase (CS) activity as a measure of aerobic potential, and lactate concentration as a measure of anaerobic potential at three different chronic acclimation temperatures (14°C, 25°C, and 31°C). By examining aerobic and anaerobic potential in both morphs, we sought to link environmental thermal flexibility to muscle metabolism. We found that the surface morphotype had higher CS activity and lower lactate concentration, suggesting an overall more efficient usage of aerobic metabolism; whereas the cave morphotype showed lower CS activity and higher lactate concentration, suggesting a stronger reliance on anaerobic pathways. We also measured white muscle histological variables that have been previously linked to whole‐animal metabolism: fiber diameter, number of nuclei per mm of fiber and myonuclear domain (MND) of both morphotypes at 25°C to examine cell‐level differences in muscle morphology. However, we found no differences in fiber diameter, number of nuclei per mm of fiber or MND between the two morphotypes. Thus, although the cellular morphology is similar in these species, the environmental differences in the evolution of the two morphs has led to differences in their metabolic profiles.

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Section: Discussionsupporting

confidence: 91%