High-speed and high-sensitivity parallel spectral-domain optical coherence tomography using a supercontinuum light source

Barrick, Jessica; Doblas, Ana; Gardner, Michael R.; Sears, Patrick R.; Ostrowski, Lawrence E.; Oldenburg, Amy L.

doi:10.1364/ol.41.005620

Cited by 36 publications

(22 citation statements)

References 19 publications

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“…With a bandwidth of 260 nm centered at 555 nm an ultrahigh resolution of 0.88 μm was achieved thereby providing access to imaging subtle structural details in healthy and pathological brain tissue such as amyloid-beta plaques. OCT based on visible light was shown to be beneficial for imaging with extremely high axial resolution as well as for performing spectroscopic measurements [ 20 – 24 , 26 , 40 , 41 ]. Ultrahigh axial resolution can be achieved by selecting a light source providing a short central wavelength and/or a very broad spectral bandwidth.…”

Section: Discussionmentioning

confidence: 99%

Spectroscopic imaging with spectral domain visible light optical coherence microscopy in Alzheimer’s disease brain samples

Lichtenegger¹,

Harper²,

Augustin³

et al. 2017

Biomed. Opt. Express

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A visible light spectral domain optical coherence microscopy system was developed. A high axial resolution of 0.88 μm in tissue was achieved using a broad visible light spectrum (425 – 685 nm). Healthy human brain tissue was imaged to quantify the difference between white (WM) and grey matter (GM) in intensity and attenuation. The high axial resolution enables the investigation of amyloid-beta plaques of various sizes in human brain tissue and animal models of Alzheimer’s disease (AD). By performing a spectroscopic analysis of the OCM data, differences in the characteristics for WM, GM, and neuritic amyloid-beta plaques were found. To gain additional contrast, Congo red stained AD brain tissue was investigated. A first effort was made to investigate optically cleared mouse brain tissue to increase the penetration depth and visualize hyperscattering structures in deeper cortical regions.

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Section: Discussionmentioning

confidence: 99%

Spectroscopic imaging with spectral domain visible light optical coherence microscopy in Alzheimer’s disease brain samples

Lichtenegger¹,

Harper²,

Augustin³

et al. 2017

Biomed. Opt. Express

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“…They provide more optical power in comparison with SLDs. For applications where high power and large light spectrum are needed, such light sources are a proper choice (e.g., [61,62]). However, for applications that require high phase stability and low noise levels, such as Doppler velocimetry and OCT angiography, SLDs remain a superior choice compared to supercontinuum lasers.…”

Section: Materials and Methods: Design Considerationsmentioning

confidence: 99%

Design and Implementation Guidelines for a Modular Spectral-Domain Optical Coherence Tomography Scanner

Atry

Rosa

Rarick

et al. 2018

International Journal of Optics

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In the past decades, spectral-domain optical coherence tomography (SD-OCT) has transformed into a widely popular imaging technology which is used in many research and clinical applications. Despite such fast growth in the field, the technology has not been readily accessible to many research laboratories either due to the cost or inflexibility of the commercially available systems or due to the lack of essential knowledge in the field of optics to develop custom-made scanners that suit specific applications. This paper aims to provide a detailed discussion on the design and development process of a typical SD-OCT scanner. The effects of multiple design parameters, for the main optical and optomechanical components, on the overall performance of the imaging system are analyzed and discussions are provided to serve as a guideline for the development of a custom SD-OCT system. While this article can be generalized for different applications, we will demonstrate the design of a SD-OCT system and representative results for in vivo brain imaging. We explain procedures to measure the axial and transversal resolutions and field of view of the system and to understand the discrepancies between the experimental and theoretical values. The specific aim of this piece is to facilitate the process of constructing custom-made SD-OCT scanners for research groups with minimum understanding of concepts in optical design and medical imaging.

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“…To our best knowledge, this is the first time that human CBF is measured using a SS-OCT system. Moreover, the result was intriguing, given that the axial resolution was previously considered as a key factor in differentiating thin ciliated epithelium layer from the rest of trachea by the community [34, 37,41,42].…”

Section: Cbf Measurement With Low Resolution Systemmentioning

confidence: 99%

Highly phase-stable 200 kHz swept-source optical coherence tomography based on KTN electro-optic deflector

Ling

Yao

Hendon

2017

Biomed. Opt. Express

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Abstract:The rapid advance in swept-source optical coherence tomography (SS-OCT) technology has enabled exciting new applications in elastography, angiography, and vibrometry, where both high temporal resolution and phase stability are highly sought-after. In this paper, we present a 200 kHz SS-OCT system centered at 1321 nm by using an electro-optically tuned swept source. The proposed system's performance was fully characterized, and it possesses superior phase stability (0.0012% scanning variability and <1 ns timing jitter) that is promising for many phase-sensitive imaging applications. Biological experiments were demonstrated within ex vivo human tracheobronchial ciliated epithelium where both the ciliary motion and ciliary beat frequency were successfully extracted.

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High-speed and high-sensitivity parallel spectral-domain optical coherence tomography using a supercontinuum light source

Cited by 36 publications

References 19 publications

Spectroscopic imaging with spectral domain visible light optical coherence microscopy in Alzheimer’s disease brain samples

Spectroscopic imaging with spectral domain visible light optical coherence microscopy in Alzheimer’s disease brain samples

Design and Implementation Guidelines for a Modular Spectral-Domain Optical Coherence Tomography Scanner

Highly phase-stable 200 kHz swept-source optical coherence tomography based on KTN electro-optic deflector

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