High-Speed DNA Sequencing by Using Mixed Poly(ethylene oxide) Solutions in Uncoated Capillary Columns

Fung, Eliza N.; Yeung, Edward S.

doi:10.1021/ac00109a002

Cited by 244 publications

(174 citation statements)

References 34 publications

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“…Capillary technology also has the potential to be engineered on a microchip scale, decreasing electrophoresis times by an order of magnitude. [131][132][133] Mathies and co-workers developed a laser-excited, confocal fluorescence imaging system for DNA sequencing or mapping fragments in a planar array of several capillaries simultaneously, a technology known as capillary array electrophoresis (CAE). 134,135 CAE offers an excellent opportunity to develop instrumentation capable of striking increases in efficiency and convenience for DNA analysis.…”

Section: Capillary Array Electrophoresis Methods For Dna Sequence Anamentioning

confidence: 99%

Parallel molecular genetic analysis

et al. 1998

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We describe recent progress in parallel molecular genetic analyses using DNA microarrays, gel-based systems, and capillary electrophoresis and utilization of these approaches in a variety of molecular biology assays. These applications include use of polymorphic markers for mapping of genes and disease-associated loci and carrier detection for genetic diseases. Application of these technologies in molecular diagnostics as well as fluorescent technologies in DNA analysis using immobilized oligonucleotide arrays on silicon or glass microchips are discussed. The array-based assays include sequencing by hybridization, cDNA expression profiling, comparative genome hybridization and genetic linkage analysis. Developments in non microarraybased, parallel analyses of mutations and gene expression profiles are reviewed. The promise of and recent progress in capillary array electrophoresis for parallel DNA sequence analysis and genotyping is summarized. Finally, a framework for decision making in selecting available technology options for specific molecular genetic analyses is presented.

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Section: Capillary Array Electrophoresis Methods For Dna Sequence Anamentioning

confidence: 99%

Parallel molecular genetic analysis

et al. 1998

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“…Interferometric characterization of ultrashort optical pulses has been performed primarily using the test-plus-reference geometry, in either the time domain [36], or the frequency domain, in which guise it is known as spectral interferometry [37][38][39].…”

Section: Self-referencing Interferometry For Characterizing Classicalmentioning

confidence: 99%