This paper presents results from a comprehensive optical study of a direct-injection spark-ignition research engine running on gasoline, iso-octane, ethanol, n-butanol and E10 fuels injected from a multi-hole injector located centrally in the combustion chamber. The analysis was based on images of spray development, spark discharge and combustion to understand the effect of injection strategy early and late in the cycle on in-cylinder phenomena. Specifically, 'single' injection strategies at different timings from early to late intake stroke, as well as multiple 'split' injection events with triple pulses in the early intake stroke or double pulses in the intake stroke and late compression stroke were investigated. The engine was run at 1500 RPM at part and full-load conditions (0.5 bar and 1.0 bar inlet plenum pressure, respectively). Engine coolant temperatures of 20-90 °C were employed to understand how the fuel's volatility was related to the phenomena observed. The sprays were imaged for a series of cycles primarily by Laser-sheet illumination on one vertical and two horizontal planes to identify three-dimensional aspects of the spray's development and its interactions with the incoming flow, valves, piston and liner. There was a clear trade-off between the earliest single injection timings in the intake stroke that reduced valve interactions but increased piston impingement and the latest single injection timings towards the end of the intake stroke which reduced piston impingement but increased valve interactions and resulted in less time for fuel evaporation and mixing before ignition timing. The triple injection strategy eliminated effects related to impingement. The spark discharge was also imaged for a series of cycles with all injection strategies and clear differences were observed. Selective combustion imaging provided insights into the flame's growth and motion with early and double early-late split injection strategies. The double early-late injection strategy demonstrated the potential for control of the mixture formation and flow field over the early flame development stage of combustion.3