High-speed laser-scanning biological microscopy using FACED

Lai, Queenie T. K.; Yip, Gwinky G. K.; Wu, Jianglai; Wong, Justin S. J.; Lo, Michael M.‐C.; Lee, Kelvin C. M.; Le, Tony T H D; So, Hayden Kwok-Hay; Ji, Na; Tsia, Kevin K.

doi:10.1038/s41596-021-00576-4

Cited by 15 publications

(9 citation statements)

References 51 publications

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“…The throughput of our system can be further increased by enlarging the FOV of the FACED 2PFM system through the optimization of the FACED module and increasing the recording length with a custom field-programmable gate array data acquisition and computing platform ( 51 ). Even faster line and full-frame scanning rates can be achieved by increasing the repetition rate of the excitation laser (e.g., 4-MHz line scanning and a 3-kHz 2D frame rate were achieved previously with a 4-MHz laser repetition rate [ 23 ]).…”

Section: Discussionmentioning

confidence: 99%

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Ultrafast two-photon fluorescence imaging of cerebral blood circulation in the mouse brain in vivo

Meng

Zhong

Zhang

et al. 2022

Proc. Natl. Acad. Sci. U.S.A.

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Significance Characterizing blood flow by tracking individual red blood cells as they move through vessels is essential for understanding vascular function. With high spatial resolution, two-photon fluorescence microscopy is the method of choice for imaging blood flow at the cellular level. However, its application is limited to a low flow speed regimen in anesthetized animals by its slow focus scanning mechanism. Using an ultrafast scanning module, we demonstrated two-photon fluorescence imaging of blood flow at 1,000 two-dimensional frames and 1,000,000 one-dimensional line scans per second in the brains of awake mice. These ultrafast measurements enabled us to study hemodynamic and fluid mechanical regimens beyond the reach of conventional methods.

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Section: Discussionmentioning

confidence: 99%

“…Detailed information on system alignment and configuration can be found in ref. 51 . Briefly, 920-nm output was generated by an optical parametric amplifier (Opera-F, Coherent; 1-MHz repetition rate) pumped by a fiber laser at 1,035 nm (Monaco 1035-40-40, Coherent; 1-MHz repetition rate) for fluorescence imaging with FITC.…”

Section: Methodsmentioning

confidence: 99%

Ultrafast two-photon fluorescence imaging of cerebral blood circulation in the mouse brain in vivo

Meng

Zhong

Zhang

et al. 2022

Proc. Natl. Acad. Sci. U.S.A.

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“…The combination of microscopy techniques also makes it possible to interrogate the activities of living systems at multiple levels with high precision. [201][202][203] It is thus envisioned that these approaches could be further improved in terms of resolution, image processing, and modelling for the study of more complicated living systems. Besides, lab-on-a-chip platforms equipped with computational tools could be further explored to provide spatiotemporal control over the mechanical and biochemical determinants.…”

Section: Discussionmentioning

confidence: 99%

Spatial confinement toward creating artificial living systems

Shang

et al. 2022

Chem. Soc. Rev.

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“…For pulse sampling, decay signals of samples are recorded using a digitizer (e.g., digital oscilloscope) with high sampling frequency and analog bandwidth (resolution of tens of ps) (Figure 6C). [ 45,46 ] However, while the TCSPC technique offers advantages of high sensitivity and high temporal resolution, this technique is generally limited by its slow data acquisition time. [ 47,48 ] On the other hand, time‐gated detection and pulse sampling techniques allow rapid and simultaneous data acquisition of all pixels.…”

Section: Time‐resolved Nir‐ii Imaging Systemsmentioning

confidence: 99%

Long‐Lived Second Near‐Infrared Luminescent Probes: An Emerging Role in Time‐Resolved Luminescence Bioimaging and Biosensing

Feng

Ko³

et al. 2022

Small Structures

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Luminescence bioimaging and biosensing in the second near‐infrared (NIR‐II) spectral window (1,000–1,700 nm wavelengths) is a newly emerging technique that is extensively used in fundamental research and clinical practice. Owing to its advantages, including deep tissue penetration and excellent temporal resolution, this technique has shown tremendous promise for target‐specific imaging and noninvasive monitoring. However, developing bioimaging modalities that enable target‐specific imaging with high resolution and sensitivity remains a great challenge today. Compared with NIR‐II fluorescence imaging, NIR‐II luminescence lifetime imaging with advantages such as lower background noises, higher sensitivity, and higher spatial resolution has opened up new avenues for deep tissue imaging and biomedical applications. Herein, various types of long‐lived NIR‐II luminescent probes, including complex dyes, rare‐earth‐doped nanoparticles, organic compound‐loaded nanoparticles, inorganic‐based dots and nanotubes, as well as the main types of time‐resolved luminescence techniques including time‐domain (e.g., time‐correlated single‐photon counting, time‐gated detection, pulse sampling, etc.) and frequency‐domain systems are first summarized. The unique advantages of long‐lived NIR‐II luminescent probes and their critical roles in bioimaging and biosensing are also highlighted. Finally, the present challenges and future directions of long‐lived NIR‐II luminescent probes in biomedical research, translational research, and (pre‐)clinical studies are discussed.

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High-speed laser-scanning biological microscopy using FACED

Cited by 15 publications

References 51 publications

Ultrafast two-photon fluorescence imaging of cerebral blood circulation in the mouse brain in vivo

Ultrafast two-photon fluorescence imaging of cerebral blood circulation in the mouse brain in vivo

Spatial confinement toward creating artificial living systems

Long‐Lived Second Near‐Infrared Luminescent Probes: An Emerging Role in Time‐Resolved Luminescence Bioimaging and Biosensing

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