High-speed Liquid Chromatographic Analysis of Drugs. II : Rapid Estimation of Berberine in Crude Drugs

Akada, Yoshinobu; Tanase, Yaichiro

doi:10.1248/yakushi1947.97.8_940

Cited by 6 publications

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“…Quaternary isoquinoline alkaloids are highly polar compounds and their analysis presents some special problems due to their strong interaction with the absorption sites of the stationary phase. The single berberine alkaloid has been quantified by HPLC in the in vivo plant (Dutschewska et al 1982) and also by using ion-pair HPLC in crude drugs and in pharmaceutical preparations (Akada and Tanase 1977;Hattori et al 1977;Akada et al 1980). The HPLC separation of quaternary alkaloids has been summarized by Verpoorte and Baerheim Svendsen in their review (1984).…”

Section: Medicinal Componentsmentioning

confidence: 99%

Medicinal and Aromatic Plants VIII

Bajaj¹

1995

Biotechnology in Agriculture and Forestry

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PrefaceThis series ofbooks on the biotechnology of Medicinal and Aromatic Plants provides a survey of the literature focusing on recent information and the state of the art in tissue culture and the in vitro production of secondary metabolites. This book, Medicinal and Aromatic Plants VIII, like the previous seven volumes published in 1988, 1989, 1991, 1993, and 1994, is unique in its approach. It comprises 26 chapters dealing with the distribution, importance, conventional propagation, micropropagation, tissue culture studies and the in vitro production of important medicinal and pharmaceutical compounds in various species of Achillea,

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Section: Medicinal Componentsmentioning

confidence: 99%

Medicinal and Aromatic Plants VIII

Bajaj¹

1995

Biotechnology in Agriculture and Forestry

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“…1) is one of the most important bioactive compounds used in Chinese herbal drugs to evaluate the quality of the Phellodendron cortex. In earlier years, several methods including acetone-UV, 3 amberlite-UV, 4 TLC-UV, 5 gas liquid chromatography (GLC), [6][7] and high-performance liquid chromatography (HPLC) 5,[8][9][10][11] were used to determine the contents of berberine in some crude drugs. But owing to the complicated components in Chinese herbal drugs, the use of UV and HPLC methods may be restricted by the lengthy analysis and the presence of potentially interfering alkaloid components.…”

Section: Introductionmentioning

confidence: 99%

Microwave Accelerated Extraction and Capillary Electrophoresis Analysis of Berberine from the Cortices of Phellodendron Wilsonii and Phellodendron Amurense

Chen

Lee

Chen

2005

Jnl Chinese Chemical Soc

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Berberine is one of the most important bioactive compounds used in Chinese herbal drugs to evaluate the quality of the Phellodendron cortex. The cortices of Phellodendron wilsonii and Phellodendron amurense, extracted by using a microwave-assisted process under a domestic microwave oven in methanol solvent and analyzed by capillary electrophoresis with the buffer solution of 82% 0.1 M aqueous sodium acetate and 18% MeOH (v/v), were demonstrated to be higher in extracting berberine contents and shorter in the process time than those extracted by using a reflux extraction in methanol solvent (for sample B, 2.27 ± 0.08% in 3 min vs. 1.92 ± 0.14% in 5 hrs, respectively). Therefore, the microwave-assisted process for extraction of berberine is more efficient, rapid and convenient than the reflux extraction and can be used for the extraction of bioactive substances from the crude Chinese drugs.

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Chelidonium majus L. (Greater Celandine): In Vitro Culture and the Production of Sanguinarine, Coptisine, and Other Isoquinoline Alkaloids

Colombo

Tomé

1995

Medicinal and Aromatic Plants VIII

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High-speed Liquid Chromatographic Analysis of Drugs. II : Rapid Estimation of Berberine in Crude Drugs

Cited by 6 publications

References 0 publications

Medicinal and Aromatic Plants VIII

Medicinal and Aromatic Plants VIII

Microwave Accelerated Extraction and Capillary Electrophoresis Analysis of Berberine from the Cortices of Phellodendron Wilsonii and Phellodendron Amurense

Chelidonium majus L. (Greater Celandine): In Vitro Culture and the Production of Sanguinarine, Coptisine, and Other Isoquinoline Alkaloids

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