2009 Annual International Conference of the IEEE Engineering in Medicine and Biology Society 2009
DOI: 10.1109/iembs.2009.5333254
|View full text |Cite
|
Sign up to set email alerts
|

High-speed spinning-disk interferometry on the BioCD for human diagnostic applications

Abstract: The BioCD is an optical biosensor that uses high-speed spinning-disk interferometry for direct detection of antigen binding in multiplexed immunoassay protein arrays. The high-frequency sampling suppresses the noise floor and enables a metrology-limited height resolution of 1 picometer for measuring surface-bound protein. In assay protocols there is a direct trade-off between multiplex degree and limit of detection. In this paper, we demonstrate quantitative assays and the scaling properties of direct detectio… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1

Citation Types

0
3
0

Year Published

2011
2011
2011
2011

Publication Types

Select...
1
1

Relationship

0
2

Authors

Journals

citations
Cited by 2 publications
(3 citation statements)
references
References 29 publications
0
3
0
Order By: Relevance
“…According to figure 6(B), the sensitivity of the EIS measurement reaches the pg ml −1 level, which is higher than the conventional measurement of μg ml −1 -ng ml −1 as measured by ELISA [3]. Our EIS method is also more sensitive than the reported biosensor, BioCD [16], which can detect at the ng ml −1 level. In addition to sensitivity, the EIS sensor can detect the phenotype before protein determination, which is important for the determination of Hp concentrations using their own calibration curve [3].…”
Section: Discussionmentioning
confidence: 80%
See 1 more Smart Citation
“…According to figure 6(B), the sensitivity of the EIS measurement reaches the pg ml −1 level, which is higher than the conventional measurement of μg ml −1 -ng ml −1 as measured by ELISA [3]. Our EIS method is also more sensitive than the reported biosensor, BioCD [16], which can detect at the ng ml −1 level. In addition to sensitivity, the EIS sensor can detect the phenotype before protein determination, which is important for the determination of Hp concentrations using their own calibration curve [3].…”
Section: Discussionmentioning
confidence: 80%
“…Biosensors for Hp phenotyping were not reported until this study. For Hp concentration determination the surface plasmon resonance (SPR) [15] or high-speed spinning-desk interferometry were to be reported [16]. Phenotyping must be performed before Hp quantization because of the structure polymorphism among the Hp types [3].…”
Section: Introductionmentioning
confidence: 99%
“…At this condition, the system becomes very sensitive and allows for the detection of small phase changes. CPI at quadrature condition have been very useful in the bio-CD technology, where thin layers of molecules are attached to a compact disk for its analysis [53,54,55]. In this case, the quadrature condition is imposed by placing the sample over a ridge of height h= 1/4 of the wave-length.…”
Section: Introductionmentioning
confidence: 99%