High Speed Video Documentation of the Mosquito Sabethes albiprivus Egg-Catapulting Oviposition Behavior (Diptera: Culicidae)

Vieira, Genilton; Bersot, Maria Ignez Lima; Pereira, Glauber Rocha; Abreu, Filipe Vieira Santos de; Nascimento-Pereira, Agostinho Cardoso; Neves, Maycon Sebastião Alberto Santos; Rosa-Freitas, María Goreti; Motta, Monique Albuquerque; Oliveira, Ricardo Lourenço de

doi:10.1007/s13744-020-00782-x

Cited by 4 publications

(6 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The oviposition behavior of Sa. albiprivus was recently recorded, and includes females throwing eggs into Sapucaia ( Lecythis pisonis ) nuts, simulating a tree hole with a small opening, which is its natural larval habitat [ 68 ]. As in Sa.…”

Section: Discussionmentioning

confidence: 99%

Yellow Fever Virus Maintained by Sabethes Mosquitoes during the Dry Season in Cerrado, a Semiarid Region of Brazil, in 2021

Oliveira¹,

Andrade

Campos

et al. 2023

Viruses

Self Cite

View full text Add to dashboard Cite

In recent decades, waves of yellow fever virus (YFV) from the Amazon Rainforest have spread and caused outbreaks in other regions of Brazil, including the Cerrado, a savannah-like biome through which YFV usually moves before arriving at the Atlantic Forest. To identify the vectors involved in the maintenance of the virus in semiarid environments, an entomological survey was conducted after confirmation of yellow fever (YF) epizootics at the peak of the dry season in the Cerrado areas of the state of Minas Gerais. In total, 917 mosquitoes from 13 taxa were collected and tested for the presence of YFV. Interestingly, mosquitoes of the Sabethes genus represented 95% of the diurnal captured specimens, displaying a peak of biting activity never previously recorded, between 4:30 and 5:30 pm. Molecular analysis identified three YFV-positive pools, two from Sabethes chloropterus—from which near-complete genomes were generated—and one from Sa. albiprivus, whose low viral load prevented sequencing. Sa. chloropterus was considered the primary vector due to the high number of copies of YFV RNA and the high relative abundance detected. Its bionomic characteristics allow its survival in dry places and dry time periods. For the first time in Brazil, Sa. albiprivus was found to be naturally infected with YFV and may have played a role as a secondary vector. Despite its high relative abundance, fewer copies of viral RNA were found, as well as a lower Minimum Infection Rate (MIR). Genomic and phylogeographic analysis showed that the virus clustered in the sub-lineage YFVPA-MG, which circulated in Pará in 2017 and then spread into other regions of the country. The results reported here contribute to the understanding of the epidemiology and mechanisms of YFV dispersion and maintenance, especially in adverse weather conditions. The intense viral circulation, even outside the seasonal period, increases the importance of surveillance and YFV vaccination to protect human populations in affected areas.

show abstract

Section: Discussionmentioning

confidence: 99%

Yellow Fever Virus Maintained by Sabethes Mosquitoes during the Dry Season in Cerrado, a Semiarid Region of Brazil, in 2021

Oliveira¹,

Andrade

Campos

et al. 2023

Viruses

Self Cite

View full text Add to dashboard Cite

show abstract

“…Following essentially the same criteria, we established a laboratory colony of Sa . albiprivus [ 30 ]. The two colonies we obtained allowed us to evaluate the vector competence of Sa .…”

Section: Discussionmentioning

confidence: 99%

“…They also allowed us to use video recordings of some of their unique behaviors in the laboratory, such as the oviposition of Sa . albiprivus in sapucaia nuts simulating a tree hole with a small entrance that is its larval habitat in the wild [ 30 ]. Here, we recorded and described for the first time the oviposition into a pierced bamboo internode cavity and the escape of newly emerged adults from it, as well as the mating behavior of Sa .…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Biological and behavioral features and colonization of the sylvatic mosquito Sabethes identicus (Diptera: Culicidae)

Lima Bersot,

Vieira,

De Moraes

et al. 2023

PLoS ONE

Self Cite

View full text Add to dashboard Cite

Mosquitoes of the genus Sabethes are exclusively sylvatic species occurring in Central and South Americas, where they play a role in the transmission of arbovirus. Sabethes identicus, a common bamboo-breeder species, has been found naturally infected with yellow fever virus. Our study aimed to describe biological and behavioral features of Sa. identicus in the laboratory, as well as establish and standardize an isolated colony for experimental assays. We tested different larval densities and evaluated larval and pupal development time, oviposition behavior, egg production, and longevity. We also shot and video-documented bionomics and behavioral aspects of the mosquitoes in the field and laboratory. A colony with more than 30 generations was achieved. Embryogenesis of Sa. identicus is completed in about three days with a 79% hatch rate, while larval and pupal development takes approximately 15–17 and nine days, respectively. The mosquito’s entire life cycle lasts approximately 30 days. Adult females could survive 71 days, and each individual laid an average of 88 eggs over their lifetime; 50% of females and males survived 37 and 24 days, respectively. Hematophagy peaks as early as the first week of emergence. The net content of a bamboo internode influenced the choice for oviposition, with the average number of eggs laid in those containing rearing water with larval and pupal exuviae being significantly higher than when they had only dechlorinated water or water with yeast. We documented for the first time an ecological association of weevils and Sa. identicus, where the oviposition of the latter depends on the weevil creating a hole in the bamboo wall for egg-laying purposes. Video recordings revealed for the first time the peculiar movements of gravid females during egg-throwing into tiny bamboo holes, as well as the laborious escape of newly emerged adults from the bamboo cavity, and mating behavior.

show abstract

“…As we all know, insect oviposition is another important aspect of reproduction, which greatly affects its population level in an ecosystem [ 1 , 17 ]. Normally, the oviposition behavior was directly observed and recorded by video [ 15 , 18 – 20 ]. The egg density and location was investigated directly under the stereomicroscopy or observed by naked eye.…”

Section: Introductionmentioning

confidence: 99%

Biological evidences for successive oogenesis and egg-laying of Matsumurasca onukii

et al. 2022

View full text Add to dashboard Cite

Tea plant (Camellia sinensis) is one of the most important horticultural cash crops, and tea green leafhopper (Matsumurasca onukii) is an extremely harmful sap-sucking pest of tea plant. Serious generation overlapping, which is mainly caused by the long oviposition period, leads to poor control effect of pesticides on this pest in the tea plantation. But the intuitive evidences of continuous oogenesis and egg-laying of this pest are still lacking, which seriously hindered the development of genetic control methods. Here, we clarified the main structures of the inner reproductive system of tea green leafhopper female adult. Oviposition behaviors were monitored as well, and six oviposition steps were recorded. According to the maturity of oocytes, the maturity stages of the reproductive system under different copulation periods were classified into 4 stages. For female adults at stage IV, mature and immature oocytes were presented simultaneously, and the developmental levels of oocytes were asynchronous among different ovarioles. The proportion of gravid females with mature oocytes significantly increased when the continuous copulation time was prolonged. In sync with the development of the ovary maturity, female adults started to slightly deposit eggs at the 5th day, and then increased dramatically. In addition, we found that, whether mature or immature, oocytes in the ovarioles always emitted green fluorescence under blue light excitation, which in turn provide solid proof for the new egg detection method from the insect physiology point of view.

show abstract

High Speed Video Documentation of the Mosquito Sabethes albiprivus Egg-Catapulting Oviposition Behavior (Diptera: Culicidae)

Cited by 4 publications

References 13 publications

Yellow Fever Virus Maintained by Sabethes Mosquitoes during the Dry Season in Cerrado, a Semiarid Region of Brazil, in 2021

Yellow Fever Virus Maintained by Sabethes Mosquitoes during the Dry Season in Cerrado, a Semiarid Region of Brazil, in 2021

Biological and behavioral features and colonization of the sylvatic mosquito Sabethes identicus (Diptera: Culicidae)

Biological evidences for successive oogenesis and egg-laying of Matsumurasca onukii

Contact Info

Product

Resources

About