High stability potentiometric urea biosensor based on enzyme attached nanoparticles

Öndeş, Baha; Akpınar, Fatma; Uygun, Murat; Muti, Mihrican; Uygun, Deniz Aktaş

doi:10.1016/j.microc.2020.105667

Cited by 46 publications

(13 citation statements)

References 29 publications

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“…The LOD value of the biosensor was found to be 7.58 μM, which means that even trace concentrations of urea can be measured at the micro level. Considering that the LOD values of even standard commercial blood urea determination kits are approximately 10 μM [43], it is understood that the proposed biosensor can even be used in the measurement of urea amounts in the blood. When the literature is reviewed, it is noticed that LOD values obtained from biosensors designed for urea are at micro‐milli molar levels.…”

Section: Resultsmentioning

confidence: 99%

Improved Sensing Performance of Amperometric Urea Biosensor by Using Platinum Nanoparticles

2021

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A novel Platinum nanoparticle (PtNPs) modified Poly(pyrrole‐co‐1‐(2‐Aminophenyl)pyrrole)/Urease film coated Au electrode was designed for amperometric detection of urea. PtNPs quantity, film density and pH were optimized and interference effect of some substances readily found in municipal wastewater and blood was investigated. The biosensor responded to urea with a measurement concentration range of 0.1 to 30 mM, a sensitivity of 31.8 μA mM−1 cm−2, a LOD of 7.58 μM, an accuracy of 104 % and a RSD% of only 0.82. It sensed the concentration of urea in the municipal sewage water with recovery of 97.6 % (n=3) and remained 78 % of its initial response at 28th day. Results confirmed that PtNPs with strong conductivity improved the electron transfer ability of the working electrode.

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Section: Resultsmentioning

confidence: 99%

Improved Sensing Performance of Amperometric Urea Biosensor by Using Platinum Nanoparticles

2021

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“…Uygun et al developed a highly stable potentiometric urea biosensor using nanoparticles. The response time and detection limit of their developed sensor were 30 s and 0.77 µM, respectively [44]. Integrating enzymes with nanomaterials has resulted in increased use of enzymes as recognition elements in biosensors [45].…”

Section: Enzyme-based Biosensorsmentioning

confidence: 99%

A Review on Biosensors and Recent Development of Nanostructured Materials-Enabled Biosensors

Naresh

Lee

2021

Sensors

1,076

427

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A biosensor is an integrated receptor-transducer device, which can convert a biological response into an electrical signal. The design and development of biosensors have taken a center stage for researchers or scientists in the recent decade owing to the wide range of biosensor applications, such as health care and disease diagnosis, environmental monitoring, water and food quality monitoring, and drug delivery. The main challenges involved in the biosensor progress are (i) the efficient capturing of biorecognition signals and the transformation of these signals into electrochemical, electrical, optical, gravimetric, or acoustic signals (transduction process), (ii) enhancing transducer performance i.e., increasing sensitivity, shorter response time, reproducibility, and low detection limits even to detect individual molecules, and (iii) miniaturization of the biosensing devices using micro-and nano-fabrication technologies. Those challenges can be met through the integration of sensing technology with nanomaterials, which range from zero- to three-dimensional, possessing a high surface-to-volume ratio, good conductivities, shock-bearing abilities, and color tunability. Nanomaterials (NMs) employed in the fabrication and nanobiosensors include nanoparticles (NPs) (high stability and high carrier capacity), nanowires (NWs) and nanorods (NRs) (capable of high detection sensitivity), carbon nanotubes (CNTs) (large surface area, high electrical and thermal conductivity), and quantum dots (QDs) (color tunability). Furthermore, these nanomaterials can themselves act as transduction elements. This review summarizes the evolution of biosensors, the types of biosensors based on their receptors, transducers, and modern approaches employed in biosensors using nanomaterials such as NPs (e.g., noble metal NPs and metal oxide NPs), NWs, NRs, CNTs, QDs, and dendrimers and their recent advancement in biosensing technology with the expansion of nanotechnology.

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“…Biosensors with this immobilized nanomaterial show high stability, achieving 90 days of storage stability and reusability up to the 170 th day. 68 Another enzyme nanocarrier, such as tobacco mosaic virus (TMV) nanoparticles, exhibits high and stable enzyme loading, which enables long-term stability and reusability. 69 Diatom microalgae also provide an excellent biosensing platform for ultrasensitive detection.…”

Section: Quality Managementmentioning

confidence: 99%

Modern analytical and bioanalytical technologies and concepts for smart and precision farming

Tsong,

Khor

2023

Anal. Methods

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High stability potentiometric urea biosensor based on enzyme attached nanoparticles

Cited by 46 publications

References 29 publications

Improved Sensing Performance of Amperometric Urea Biosensor by Using Platinum Nanoparticles

Improved Sensing Performance of Amperometric Urea Biosensor by Using Platinum Nanoparticles

A Review on Biosensors and Recent Development of Nanostructured Materials-Enabled Biosensors

Modern analytical and bioanalytical technologies and concepts for smart and precision farming

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