High surface hydrophobicity ofStaphylococcus aureus as revealed by hydrophobic interaction chromatography

Johsson, Per; Wadström, Torkel

doi:10.1007/bf01573707

Cited by 52 publications

(19 citation statements)

References 28 publications

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“…The results of previous studies of the relationship between the presence of protein A and hydrophobicity have been ambiguous (10,14). Those in the present study demonstrated that two protein A-deficient strains and their parent protein A-positive strains were hydrophobic.…”

Section: Discussioncontrasting

confidence: 51%

Surface Properties of Staphylococcus aureus Affecting Chemiluminescence Response of Human Phagocytes

Yamada

Matsumoto

1990

Microbiology and Immunology

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To assess the surface properties of Staphylococcus aureus affecting the response of human phagocytes, the effects of the organisms with different surface properties on the chemiluminescence (CL) response of human phagocytes were examined. The magnitude of the phagocytic CL response to hydrophobic strains was significantly greater than that to hydrophilic strains, while no significant difference in the CL response was seen between protein A-deficient strains and their parent strains.The CL response to the hydrophilic organisms prepared from a hydrophobic strain by trypsin treatment decreased significantly.These results suggest that the phagocytic CL response to staphylococci depends on the hydrophobicity of the surface, but not on the presence of protein A. Two protein A-deficient strains which were isolated from protein A-positive strains showed identical hydrophobicity with their parent strains. All of the hydrophilic strains isolated from hydrophobic strains possessed protein A identical to that of their parent strains.Moreover, a hydrophilic strain could be isolated from a protein A-deficient, hydrophobic strain. These results strongly suggest that protein A is not solely responsible for the surface hydrophobicity of S. aureus.

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Section: Discussioncontrasting

confidence: 51%

Surface Properties of Staphylococcus aureus Affecting Chemiluminescence Response of Human Phagocytes

Yamada

Matsumoto

1990

Microbiology and Immunology

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“…S. aweus grown exponentially but relatively slowly (DT 60 min) was far less hydrophobic, particularly when iron-stressed, than when grown exponentially but relatively quickly (DT 24 min). A correlation was found as cell-bound PrA, a significant contributor to SH [13], was reduced in the slow-grown phenotypes, especially in the TSB -Fe cocci. Significantly, the latter had CA values N 5-6" less than those of human PMN (18-19" [2,14]), implying, from interfacial tension principles [2], that it would be the least easily phagocytosed of the phenotypes.…”

Section: Sh and Pra Expressionmentioning

confidence: 88%

Bacterial doubling time modulates the effects of opsonisation and available iron upon interactions betweenStaphylococcus aureusand human neutrophils

Domingue

Costerton

Brown

1996

FEMS Immunol Med Microbiol

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Staphylococcus aureus was grown exponentially at two doubling times (DT), one related to in vivo (DT 60 min) and one typical of laboratory conditions (DT 24 min), and under iron-poor and iron-rich conditions. Relative to the fast-grown phenotypes, both slow-grown phenotypes exhibited low surface hydrophobicity and low protein A expression, induced poorly in non-opsonised and opsonised chemiluminescence, and survived well in whole blood killing. In particular, slow-grown, iron-poor cocci demonstrated enhanced survival in whole blood killing which correlated with a significant reduction in their association with polymorphonuclear leukocytes, compared to the three other phenotypes; iron sufficiency increased the ability to stimulate polymorphonuclear leukocytes irrespective of opsonisation status. Staphylococcal DT may, by influencing surface hydrophobicity, modify interactions with immune system components.

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“…The most common methods to measure microbial surface hydrophobicity are; partitioning in aqueous two phase systems, [16] hydrophobic interaction chromatography, [17] salt aggregation, [18] polystyrene microsphere attachment, [19] contact angle measurements, [9] and microbial adhesion to hydrocarbons. The most common methods to measure microbial surface hydrophobicity are; partitioning in aqueous two phase systems, [16] hydrophobic interaction chromatography, [17] salt aggregation, [18] polystyrene microsphere attachment, [19] contact angle measurements, [9] and microbial adhesion to hydrocarbons.…”

Section: Introductionmentioning

confidence: 99%

Effect of Starvation on Resuscitation and the Surface Characteristics of Bacteria

Sanin

2003

Journal of Environmental Science and Health, Part A

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Resuscitation behavior of bacteria after starvation for carbon and nitrogen was investigated. In addition effect of carbon and nitrogen starvation conditions on the surface characteristics and adhesive properties of bacteria were studied. Two pure culture herbicide degrading bacteria were used in the study: Pseudomonas sp. strain A, and Rhodococcus corallinus strain 11. These bacteria are known to degrade cyanuric acid which is a derivative of s-triazine, a common herbicide used widely. Selected bacteria were starved for carbon (glucose) and nitrogen (cyanuric acid) in different bioreactors and their physiological responses to starvation and resuscitation were measured. Different resuscitation responses were observed under different starvation conditions such that long lag phase was, observed for Rhodococcus corallinus strain 11 subjected to cyanuric acid starvation. Slow exponential growth rates were calculated for both microorganisms subjected to cyanuric acid starvation. The surface properties of both microorganisms were investigated using MATH test with two different hydrocarbons (hexadecane and octane). Hexadecane was observed to be the best organic attachment phase for these tests. Surface hydrophobiciy for all the microorganisms stayed unchanged during carbon starvation conditions. Significant decrease in hydrophobicity was observed for both cultures starved for nitrogen. When the hydrophobicity of the cultures decreased, the attachment capabilities of the microorganisms decreased. The decrease in attachment capabilities is a result of highly hydrated extracellular polysaccharides produced in the presence of carbon in the medium. Results of this study can be used as control tools in soil remediation applications.

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High surface hydrophobicity ofStaphylococcus aureus as revealed by hydrophobic interaction chromatography

Cited by 52 publications

References 28 publications

Surface Properties of Staphylococcus aureus Affecting Chemiluminescence Response of Human Phagocytes

Surface Properties of Staphylococcus aureus Affecting Chemiluminescence Response of Human Phagocytes

Bacterial doubling time modulates the effects of opsonisation and available iron upon interactions betweenStaphylococcus aureusand human neutrophils

Effect of Starvation on Resuscitation and the Surface Characteristics of Bacteria

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