High‐Temperature Spray‐Dried Polymer/Bacteria Microparticles for Electrospinning of Composite Nonwovens

Reich, Steffen; Kaiser, Patrick; Mafi, Mahsa; Schmalz, Holger; Rhinow, Daniel; Freitag, Ruth; Greiner, Andreas

doi:10.1002/mabi.201800356

Cited by 11 publications

(7 citation statements)

References 19 publications

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“…The SEM analysis revealed that studied MPs exhibit a spherical shape with size heterogeneity in the range of 1 to 20 µm (Fig. 1 A), similarly as showed by Reich et al 60 . The presence of additives did not affect the size of developed microparticles, whereas the addition of Pluronic F-127 caused a slight collapse of their structure, similar to the report of Reich et al 60 .…”

Section: Resultssupporting

confidence: 83%

Spray-dried pH-sensitive chitosan microparticles loaded with Mycobacterium bovis BCG intended for supporting treatment of Helicobacter pylori infection

Gonciarz,

Brzeziński,

Orłowska

et al. 2024

Sci Rep

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Gram-negative spiral-shaped Helicobacter pylori (Hp) bacteria induce the development of different gastric disorders. The growing resistance of Hp to antibiotics prompts to search for new therapeutic formulations. A promising candidate is Mycobacterium bovis BCG (BCG) with immunomodulatory properties. Biodegradable mucoadhesive chitosan is a good carrier for delivering BCG mycobacteria to the gastric mucosal environment. This study aimed to show whether BCG bacilli are able to increase the phagocytic activity of Cavia porcellus—guinea pig macrophages derived from the bone marrow towards fluorescently labeled Escherichia coli. Furthermore, to encapsulate live BCG bacilli, in spray-dried chitosan microparticles (CHI-MPs), and assess the pH-dependent release of mycobacteria in pH conditions mimicking gastric (acidic) or gut (alkaline) milieu. Microparticles (MPs) were made of chitosan and coated with Pluronic F-127-(Plur) or N-Acetyl-d-Glucosamine-(GlcNAc) to increase the MPs resistance to low pH or to increase anti-Hp effect, respectively. Spray-drying method was used for microencapsulation of live BCG. The biosafety of tested CHI-MPs has been confirmed using cell models in vitro and the model of guinea pig in vivo. The CHI-MPs loaded with BCG released live mycobacteria at pH 3.0 (CHI-GlcNAc-MPs) or pH 8.0. (CHI-Plur-MPs). The CHI-MPs loaded with live BCG can be used for per os inoculation of Cavia porcellus to check the effectiveness of delivered mycobacteria in increasing anti-H. pylori host response.

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Section: Resultssupporting

confidence: 83%

Spray-dried pH-sensitive chitosan microparticles loaded with Mycobacterium bovis BCG intended for supporting treatment of Helicobacter pylori infection

Gonciarz,

Brzeziński,

Orłowska

et al. 2024

Sci Rep

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“…and by a Raman‐atomic force microscopy (AFM) measurement. [ 22 ] In this study, the presence of M. luteus inside the PVA microparticles was shown. The diameter size of the PVA microparticles with encapsulated M. luteus is in the range of 2.58 ± 0.89 µm (Figure S1, Supporting Information).…”

Section: Resultsmentioning

confidence: 74%

“…introduced the encapsulation of bacteria in polymer microparticles by spray drying of gram‐positive and negative bacteria in different polymer solutions. [ 22 ]…”

Section: Introductionmentioning

confidence: 99%

Bioremediation of Copper‐Ions by Polymer Encapsulated and Immobilized Micrococcus Luteus

Mafi

Greiner

2021

Macromolecular Bioscience

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Bioremediation of copper (Cu2+) with immobilized Micrococcus luteus in polymer matrices has been broadly studied for a wide range of applications including wastewater treatment. Herein, the bioremediation efficiency based on modified immobilization techniques and by the addition of Cu2+ is investigated. Porous composite nonwovens with living M. luteus (living polymer composites) are prepared by encapsulation of the bacterial cells in poly(vinyl alcohol) (PVA) microparticles (M. luteus/PVA microparticles) produced by spray drying method. The M. luteus/PVA microparticles are chemically cross‐linked. The hydrogel microparticles with encapsulated M. luteus are embedded in a nonwoven of poly (lactic acid) (PLA) electrospun short fibers provided by wet‐laid method. Two different models of composite nonwovens are reported, in which the place position of the hydrogel PVA microparticles with encapsulated M. luteus and PLA nonwoven can affect the bioremediation process.

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“…In our previous work, we showed the encapsulation of bacteria in PVA microparticles by advanced characterization method, such as Raman‐AFM. [ 19 ] Since some of these PVA microparticles also contained dead M. luteus , we analyzed the survival of M. luteus in additional steps using the live/dead analysis test and by differentiating the live and dead cells (microparticles with dead M. luteus cells [sample 3] were used as control samples for live/dead test).…”

Section: Resultsmentioning

confidence: 99%

“…[15,17,18] Reich et al reported the encapsulation of two different strains of bacteria in polymer particles prepared by spray drying. [19] Despite this, the uncontrolled release of bacteria from the particles is still a challenge, as is the uptake of nutrients for the bacteria in the particles. Indeed, bacteria were released from pure PVA particles immediately after contact with aqueous nutrient media.…”

Section: Introductionmentioning

confidence: 99%

Poly(Vinyl Alcohol)‐Hydrogel Microparticles with Soft Barrier Shell for the Encapsulation of Micrococcus luteus

Mafi

Kushmaro

Greenblatt

et al. 2021

Macromolecular Bioscience

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The encapsulation of bacteria in polymers results in hybrid materials that are essential for the long‐term biological activity of bacteria and formulations in practical applications. Here, the problem of bacterial escape and the exchange of metabolism products from hydrogel microparticles within an aqueous environment are addressed. Bacteria are encapsulated in chemically cross‐linked poly(vinyl alcohol) (PVA) hydrogel‐microparticles followed by their encapsulation in a pH‐responsive and soft antibacterial shell of poly(N,N‐diethylamino ethyl methacrylate) (PDEAEMA). This polymer shell acts selectively with regards to the mass transport in and out of the microparticle core and is affected by environmental parameters, such as pH and antibacterial effect. The pH‐responsive PDEAEMA shell forms an open porous structure that accelerates nutrient transfer into the PVA core containing living Micrococcus luteus (M. luteus). Results show that the antibacterial effect of PDEAEMA retards the escape of bacteria up to 35 days when the shell is open. Additionally, the permeation of a small molecule into the gel, for example, methylene blue dye through the core/open‐shell structure, certifies a flexible barrier for mass transport, which is required in the long term for the biological activity of encapsulated M. luteus.

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High‐Temperature Spray‐Dried Polymer/Bacteria Microparticles for Electrospinning of Composite Nonwovens

Cited by 11 publications

References 19 publications

Spray-dried pH-sensitive chitosan microparticles loaded with Mycobacterium bovis BCG intended for supporting treatment of Helicobacter pylori infection

Spray-dried pH-sensitive chitosan microparticles loaded with Mycobacterium bovis BCG intended for supporting treatment of Helicobacter pylori infection

Bioremediation of Copper‐Ions by Polymer Encapsulated and Immobilized Micrococcus Luteus

Poly(Vinyl Alcohol)‐Hydrogel Microparticles with Soft Barrier Shell for the Encapsulation of Micrococcus luteus

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