High throughput Agrobacterium-mediated switchgrass transformation

Li, Ruyu; Qu, Rongda

doi:10.1016/j.biombioe.2010.11.025

Cited by 79 publications

(128 citation statements)

References 36 publications

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“…Proline has been used at low concentrations (100-500 mg l −1 ) in combination with casein hydrolysates (Burris et al 2009;Ramamoorthy and Kumar 2012) to induce Type II calli in switchgrass. Li and Qu (2011) used proline alone at high concentration (2 g l −1 ) for friable embryogenic callus induction, and reported that all regenerated plants were albino after calli of cultivar Alamo were subcultured for 14 months. These results suggested that PVCc medium containing casamino acids would be a better medium than prolinecontaining medium to improve shoot regeneration without albino induction.…”

mentioning

confidence: 99%

Long-term maintenance of high regeneration ability of switchgrass embryogenic callus

Ogawa

2015

Plant Biotechnology

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Switchgrass (Panicum virgatum L.) is one of the most important crops for forage and bioenergy, and embryogenic callus is an important material for molecular breeding of this species. In this study, the longevity of caryopsisderived compact Type I callus of switchgrass was investigated during 24 months. The regeneration ability of the callus was gradually reduced after 18 months of subculture, but remained at a relatively high level after 24 months. In addition, albino formation was not induced throughout the 24-month subculture. Casamino acids improved the regeneration ability of embryogenic calli without apparent morphological change or albino induction, while proline induced friable Type II calli as well as albino shoots. Cell straining treatment coupled with medium containing casamino acids led to 4-fold higher regeneration ability. The ploidy levels of 24-month-old calli were similar to seedling explants. The present results indicate that caryopsis-derived Type I callus is stable and could be maintained long-term, and thus would be a useful source for genetic transformation of switchgrass.

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confidence: 99%

Long-term maintenance of high regeneration ability of switchgrass embryogenic callus

Ogawa

2015

Plant Biotechnology

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“…Transgene inheritance and phenotypic expression in progeny derived from Agrobacteriummediated transformation were observed in some of these reports (Li & Qu, 2011;Somleva et al, 2002;Xi et al, 2009). …”

Section: Switchgrass Transformation Systemsmentioning

confidence: 96%

“…For stable transformation of switchgrass, the most noteworthy Agrobacterium strain so far has been EHA105; two different research groups found it to be more effective than other strains, such as AGL1, GV3101, and LBA4404 (Song et al, 2011;Xi et al, 2009). Li & Qu (2011) recently developed the most high-through-put system to date, with a stable transformation efficiency of over 90%. Although most previous transformation systems employed the cultivar 'Alamo', the authors a found higher transformation efficiency with the cultivar 'Performer'.…”

Section: Switchgrass Transformation Systemsmentioning

confidence: 99%

“…Although most previous transformation systems employed the cultivar 'Alamo', the authors a found higher transformation efficiency with the cultivar 'Performer'. Their modifications included infection under vacuum, cocultivation in desiccation conditions, resting between co-cultivation and selection, and Lproline supplementation in the callus culture and selection media (Li & Qu, 2011). Interestingly, this system also employed Agrobacterium strain EHA105, suggesting that it may be highly compatible with 'Performer'.…”

Section: Switchgrass Transformation Systemsmentioning

confidence: 99%

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Molecular Breeding of Grasses by Transgenic Approaches for Biofuel Production

Takahashi¹,

Takamizo²

2012

Transgenic Plants - Advances and Limitations

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“…Hygromycin B concentrations in media of more than 50 mg/L have been used in transformation protocols for some monocotyledonous plants (Dong and Qu 2005;Ge et al 2006;Li and Qu 2011). However, nearly all L. rigidum calli showed necrosis within 1 week and almost all calli infected with A. tumefaciens died within 2 weeks of inoculation when subcultured onto media containing 50 mg/L hygromycin B.…”

Section: Establishment Of a Transformation System For Annual Ryegrassmentioning

confidence: 99%

Agrobacterium-mediated transformation of Lolium rigidum Gaud.

Ran

Patron

et al. 2014

Plant Cell Tiss Organ Cult

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Lolium rigidum Gaud. is an annual grass grown for forage but also an economically damaging crop weed. A single genotype somatic embryogenic callus line, VLR1-60, was identified from a herbicide susceptible L. rigidum population, VLR1, and proved to be amenable to Agrobacterium tumefaciens-mediated transformation. Somatic embryogenic calli were continuously induced from the meristematic region of VLR1-60 plants multiplied in vitro and the basic tolerance level of VLR1-60 to hygromycin B was determined. A hygromycin phosphotransferase gene was used as a selectable marker for hygromycin B selection. Somatic embryogenic calli derived from in vitro grown vegetative tillers were co-cultivated with the A. tumefaciens strain EHA105 harbouring binary vector carrying reporter genes and selectable marker in the presence of acetosyringone for 3 days. Inoculated calli were recovered on callus proliferation medium containing Timentin TM but lacking hygromycin and were then subcultured onto media with hygromycin concentrations increased progressively through time for selection of transformed plant cells. Putative transgenic plants were recovered and integration of transgenes was confirmed by Southern hybridization analysis and by detection of DsRed or GUS activity in transgenic plants. The frequency of plant transformation was 1.3 %. The ability to transform L. rigidum will provide opportunities for functional characterization of genes to improve forage quality and increase our understanding of the evolution of herbicide resistance and of the basic genetics underlying traits that make L. rigidum a damaging crop weed.

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High throughput Agrobacterium-mediated switchgrass transformation

Cited by 79 publications

References 36 publications

Long-term maintenance of high regeneration ability of switchgrass embryogenic callus

Long-term maintenance of high regeneration ability of switchgrass embryogenic callus

Molecular Breeding of Grasses by Transgenic Approaches for Biofuel Production

Agrobacterium-mediated transformation of Lolium rigidum Gaud.

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