High-throughput analysis of biomolecular interactions and cellular responses with resonant waveguide grating biosensors

Ye, Fang; Fang, Jack; Tran, Elizabeth; Xie, Xinying; Hallstrom, Michael; Frutos, Anthony G.

doi:10.1017/cbo9780511626531.011

Cited by 5 publications

(11 citation statements)

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“…They have now been developed to accommodate cell-based assays [11]. Although there are numerous 'optical biosensor' instruments available on the market, they essentially all exploit the properties of an evanescent wave to quantify a biological event [31]. The term 'evanescent' is derived from Latin meaning 'vanishing.'…”

Section: Optical Biosensorsmentioning

confidence: 99%

Using label-free screening technology to improve efficiency in drug discovery

Halai

Cooper

2012

Expert Opinion on Drug Discovery

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The limitations associated with the use of transfected cell lines and the use of label-based assays are gradually being realized. As such, greater emphasis is being placed on label-free biophysical techniques using native cell lines. The introduction of 96- and 384-well plate label-free systems is helping to broker a wider acceptance of these approaches in high-throughput screening. However, potential users of the technologies remain skeptical, primarily because the physical basis of the signals generated, and their contextual relevance to cell biology and signal transduction, has not been fully elucidated. Until this is done, these new technology platforms are more likely to complement, rather than replace, traditional screening platforms.

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Section: Optical Biosensorsmentioning

confidence: 99%

Using label-free screening technology to improve efficiency in drug discovery

Halai

Cooper

2012

Expert Opinion on Drug Discovery

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“…This approach can readily be extrapolated to other difficult to assay or complex targets that might include nucleic acid interacting targets (e.g., topoisomerases, polymerases, telomerases, and integrases), catalytically inactive enzymes (e.g., unactivated kinases), or catalytically unstable enzymes (e.g., cyclooxygenase) and protein-protein interactions. 5,12,13 Time Courses and K d in Plate-Based Biosensors, as Compared to Flow Biosensors Flow-based biosensors, such as the Biacore implementation of SPR, have become the gold standard for label-free measurements of association and dissociation phases. Indeed, SPR studies for direct binding of ligands to nuclear receptor ligand-binding domains have been reported, [16][17][18] though these methods have not been reported with ERRg.…”

Section: Mechanistic Considerationsmentioning

confidence: 99%

“…[2][3][4][5][6][7][8][9][10][11][12][13] Current HTS technologies are almost all based on industry-standard microplates. Traditional label-free approaches such as ITC and surface plasmon resonance (SPR) do not comply with this architecture.…”

Section: Introductionmentioning

confidence: 99%

“…3,[6][7][8] However, the recently commercialized photonic crystal-based biosensors are manufactured in standard microplate formats of 96, 384 and 1,536 wells. [9][10][11][12][13] These microplates can be read in easy-to-use benchtop plate readers, thereby forming a simple sensitive biosensor system, fully compatible with standard liquid and microplate handling automation. Biomass changes at the biosensor surface cause alterations in local refractive index (as with SPR), detected by precisely measurable shifts in the peak wavelength value (PWV) of sensor-specifically reflected light.…”

Section: Introductionmentioning

confidence: 99%

“…Such applications span from the screening of compound libraries to hit (in)validation, and mechanistic pharmacology of leads and from agonist trafficking to receptor panning. 4,[9][10][11][12][13] ABBREVIATIONS: b-RIP, biotinylated peptide derived from RIP140; CV, coefficient of variation; ERRg, estrogen-related receptor gamma; LBD, ligand-binding domain; PWV, peak-wavelength value; SD, standard deviation; SPR, surface plasmon resonance; DPWV, the change in peak-wavelength value.…”

Section: Introductionmentioning

confidence: 99%

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Validation of an Optical Microplate Label-Free Platform in the Screening of Chemical Libraries for Direct Binding to a Nuclear Receptor

Vela

Lowe²,

Gerstenmaier³

et al. 2011

ASSAY and Drug Development Technologies

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Optical microplate-based biosensors combine the advantages of label-free detection with industry-standard assay laboratory infrastructure and scalability. A plate-based label-free platform allows the same basic platform to be used to quantify molecular interactions of macromolecules and to screen and characterize drug-like small-molecule interactions. The ligand-binding domain of orphan estrogen-related nuclear receptor-γ (ERRγ) is utilized, as a model system of a challenging type of target, to illustrate the rapid development and utility of a range of biochemical assay formats on these biosensors. Formats in which either the domain, or a peptide derived from its cognate corepressor, RIP140, were immobilized were utilized. The direct binding of small drug molecules to the domain was characterized using immobilized domain. Subsequent addition of peptide distinguished whether compounds acted as either antagonists of peptide binding, or as agonists promoting a ternary complex. The format with peptide immobilized gave a more sensitive procedure for establishing the effect of compounds on the domain-peptide interaction. Using a direct-binding format, a diverse chemical library of 1,408 compounds in DMSO was screened for ability to bind to biosensors coated with ERRγ ligand-binding domain. Hits were then characterized using the other biosensor assay formats. The standard requirements for a full primary screening campaign were fulfilled by the acceptable hit-rate, quality-performance parameters, and throughput of the direct-binding assay format. Such a format allows direct screening of targets, such as orphan receptors, without the requirement for prior knowledge of a validated ligand.

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Recent Advances in Resonant Waveguide Gratings

Quaranta

Basset

Martin

et al. 2018

Laser & Photonics Reviews

322

199

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Resonant waveguide gratings (RWGs), also known as guided mode resonant (GMR) gratings or waveguide‐mode resonant gratings, are dielectric structures where these resonant diffractive elements benefit from lateral leaky guided modes from UV to microwave frequencies in many different configurations. A broad range of optical effects are obtained using RWGs such as waveguide coupling, filtering, focusing, field enhancement and nonlinear effects, magneto‐optical Kerr effect, or electromagnetically induced transparency. Thanks to their high degree of optical tunability (wavelength, phase, polarization, intensity) and the variety of fabrication processes and materials available, RWGs have been implemented in a broad scope of applications in research and industry: refractive index and fluorescence biosensors, solar cells and photodetectors, signal processing, polarizers and wave plates, spectrometers, active tunable filters, mirrors for lasers and optical security features. The aim of this review is to discuss the latest developments in the field including numerical modeling, manufacturing, the physics, and applications of RWGs. Scientists and engineers interested in using RWGs for their application will also find links to the standard tools and references in modeling and fabrication according to their needs.

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High-throughput analysis of biomolecular interactions and cellular responses with resonant waveguide grating biosensors

Cited by 5 publications

References 0 publications

Using label-free screening technology to improve efficiency in drug discovery

Using label-free screening technology to improve efficiency in drug discovery

Validation of an Optical Microplate Label-Free Platform in the Screening of Chemical Libraries for Direct Binding to a Nuclear Receptor

Recent Advances in Resonant Waveguide Gratings

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