High-throughput autoantibody analysis in malignant pleural effusion and tuberculosis pleural effusion

Yi, Facheng; Zhang, Xin

doi:10.1097/md.0000000000017253

Cited by 4 publications

(1 citation statement)

References 26 publications

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“…A recent study screened out a panel of naturally occurring antibodies that were altered in AD patients [12], suggesting that humoral autoimmunity may participate in the pathogenesis of AD. Naturally occurring antibodies against Bim (NAbs-Bim) exist in human blood and may be involved in the pathogenesis of diseases such as malignant pleura effusion [13]. We found in this study that circulating NAbs-Bim were decreased in AD patients, with the pathophysiological functions of these antibodies to be demonstrated.…”

Section: Introductionmentioning

confidence: 64%

Naturally Occurring Antibodies Against Bim Are Decreased in Alzheimer’s Disease and Attenuate Ad-type Pathologies in a Mouse Model

Jian

Fan

Tian

et al. 2021

Preprint

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Background Alzheimer’s disease (AD) is the most popular neurodegenerative disease affecting cognitive functions of the elderly population. Neuronal apoptosis is an important pathological process during the development of AD. The Bcl-2-interacting mediator of cell death (Bim) mediates Amyloid-beta (Aβ)-induced neuronal apoptosis. Naturally occurring antibodies against Bim (NAbs-Bim) exist in human blood, with their levels and functions unknown in AD. Methods This study investigated the clinical relevance of plasma NAbs-Bim to AD in 55 AD patients, 28 patients with non-AD dementia, and 70 cognitively normal subjects. Furthermore, the pathophysiological functions of NAbs-Bim were explored in APP/PS1 mice and SY5Y cell lines overexpressing human amyloid precursor protein (APP). Results We found that plasma levels of NAbs-Bim were lower in AD patients in comparison with patients with non-AD dementia and cognitively normal controls. Plasma levels of NAbs-Bim were negatively associated with brain amyloid burdens and positively associated with cognitive functions. NAbs-Bim purified from intravenous immunoglobulin rescued behavioral deficits of APP/PS1 mice. NAbs-Bim ameliorated Aβ deposition, Tau hyperphosphorylation, microgliosis and neuronal apoptosis in APP/PS1 mice. In vitro investigations demonstrated that NAbs-Bim exerted neuroprotective effects against AD through neutralizing Bim-directed neuronal apoptosis and amyloidogenic processing of amyloid precursor protein. Conclusions The decrease of NAbs-Bim might contribute to the pathogenesis of AD and immunotherapies targeting Bim may hold promise for the treatment of AD.

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Section: Introductionmentioning

confidence: 64%

Naturally Occurring Antibodies Against Bim Are Decreased in Alzheimer’s Disease and Attenuate Ad-type Pathologies in a Mouse Model

Jian

Fan

Tian

et al. 2021

Preprint

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Naturally-Occurring Antibodies Against Bim are Decreased in Alzheimer’s Disease and Attenuate AD-type Pathology in a Mouse Model

et al. 2022

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Natural antibodies targeting LPS in pleural effusions of various etiologies

Sarrigeorgiou,

Rouka,

Kotsiou

et al. 2024

American Journal of Physiology-Lung Cellular and Molecular Phys

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Background: Respiratory infection, cancer and heart failure can cause abnormal accumulation of fluid in the pleural cavity. The immune responses within the cavity are orchestrated by leucocytes that reside in the serosal associated lymphoid tissue. Natural antibodies (NAbs) are abundant in the serum having a major role in systemic and mucosal immunity, however their occurrence in pleural fluid remains an open question. Our aim herein was to detect and measure the levels of NAbs targeting LPS of M, G, and A class in both, the pleural fluid and the serum of 78 patients with pleural effusions (PEs) of various etiologies. Methods: The values of anti-LPS NAb activity were extracted through a normalization step regarding the total IgM, IgG and IgA levels and in addition the ratio of PF/S values were analyzed further with other critical biochemical parameters from biopsies. Results: Anti-LPS NAbs of all Ig classes were detected in most of the samples, while a significant increase of anti-LPS activity was observed in infectious and non-infectious compared to malignant PEs. Multivariate linear regression confirmed a negative correlation of IgM and IgA anti-LPS PF/S ratio with malignancy. Moreover, anti-LPS NAbs PF/S measurements led to increased positive and negative predictive power in ROC curves generated for the discrimination between benign and malignant PEs. Conclusions: Our results highlight a potential role of anti-LPS NAbs in the pleural cavity that should be further explored.

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High-throughput autoantibody analysis in malignant pleural effusion and tuberculosis pleural effusion

Abstract: Supplemental Digital Content is available in the text

Cited by 4 publications

References 26 publications

Naturally Occurring Antibodies Against Bim Are Decreased in Alzheimer’s Disease and Attenuate Ad-type Pathologies in a Mouse Model

Naturally Occurring Antibodies Against Bim Are Decreased in Alzheimer’s Disease and Attenuate Ad-type Pathologies in a Mouse Model

Naturally-Occurring Antibodies Against Bim are Decreased in Alzheimer’s Disease and Attenuate AD-type Pathology in a Mouse Model

Natural antibodies targeting LPS in pleural effusions of various etiologies

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