High Throughput Bioassays Using Nanoparticles

Gómez‐Hens, A.; Fernández-Romero, J.M.; Aguilar-Caballos, M.P.

doi:10.2174/138620710791054240

Cited by 4 publications

(3 citation statements)

References 73 publications

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“…Miniaturization with nanotechnology has also improved the speed and efficiency of existing bioassays and spurred development of new measurement techniques to allow the use of smaller samples and smaller amounts of reagents, less processing, more rapid interrogation, and lower limits of detection. 1 These methods take advantage of the unique properties of nanostructures, which exhibit optical, chemical, structural, and electronic behaviors that are not observed for bulk material.…”

Section: Introductionmentioning

confidence: 99%

Surface-Enhanced Raman Scattering Detection and Tracking of Nanoprobes: Enhanced Uptake and Nuclear Targeting in Single Cells

et al. 2010

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We describe the development and application of a co-functionalized nanoprobe and biodelivery platform combining a nuclear targeting peptide (NTP) for improved cellular uptake and intracellular targeting with p-mercaptobenzoic acid (pMBA) as a surface-enhanced Raman scattering (SERS) reporter for tracking and imaging. The nuclear targeting peptide, an HIV-1 protein-derived TAT sequence, has been previously shown to aid entry of cargo through the cell membrane via normal cellular processes, and furthermore, to localize small cargo to the nucleus of the cell. Previous work in our lab has verified cell uptake and distribution of the nanoprobes in clinically relevant mouse and human cell lines. In this work, two-dimensional SERS mapping was used to track the spatial and temporal progress of nanoparticle uptake in PC-3 human prostate cells and to characterize localization at various time points, demonstrating the potential for an intracellularly targeted multiplexed nanobiosensing system with excellent sensitivity and specificity. Silver nanoparticles co-functionalized with the TAT peptide showed greatly enhanced cellular uptake over the control nanoparticles lacking the targeting moiety. The ability to detect and monitor nanoprobe trafficking using SERS spectroscopy offers an improved alternative over previous tracking and detection methods such as light microscopy and fluorescence methods. The development of multifunctional nanoconstructs for intracellular delivery has potential clinical applications in early detection and selective treatment of disease in affected cells. Other applications include use in basic research aimed at understanding the inner workings of living cells and how they respond to chemical and biological stimuli.

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Section: Introductionmentioning

confidence: 99%

Surface-Enhanced Raman Scattering Detection and Tracking of Nanoprobes: Enhanced Uptake and Nuclear Targeting in Single Cells

et al. 2010

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“…The ability to reliably and reproducibly conjugate antibodies to nanoparticles is key to developing platforms for next generation technologies from diagnostics to therapeutics . Applications include single molecule tracking, cell sorting, bioseparations, quantitative assays for protein detection, enzyme immobilization, protein purification, immunoasays, along with in vivo applications such as targeted contrast agents for imaging and targeted drug/gene delivery. − …”

mentioning

confidence: 99%

Universal Antibody Conjugation to Nanoparticles Using the Fcγ Receptor I (FcγRI): Quantitative Profiling Of Membrane Biomarkers

Kim

Galloway²,

Lee³

et al. 2014

Bioconjugate Chem.

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Antibodies are a class of molecules widely used in bioengineering and nanomedicine for applications involving protein recognition and targeting. Here we report an efficient method for universal conjugation of antibodies to lipid-coated nanoparticles using radially oriented FcγRIs. This method is performed in physiological solution with no additional coupling reagents, thereby avoiding problems with antibody stability and functionality. Coupling to the Fc region of the antibody avoids aggregation and polymerization allowing high yield. In addition, the antibody is oriented perpendicular to the surface so that the binding sites are fully functional. Using this method we demonstrate quantitative profiling of a panel of four membrane-bound cancer biomarkers (claudin-4, mesothelin, mucin-4, and cadherin-11) on four cell lines (Panc-1, MIA PaCa-2, Capan-1, and HPDE). We show that by designing the lipid coating to minimize aggregation and nonspecific binding, we can obtain absolute values of biomarker expression levels as number per unit area on the cell surface. This method is applicable to a wide range of technologies, including solution based protein detection assays and active targeting of cell surface membrane biomarkers.

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“…Phthalates are available in free form and not chemically bound in plastic. As a result, they can potentially migrate from packaging materials into our food and beverages and cause contamination during production and storage (Gomez-Hens & Aguilar-Caballos, 2003). The compound has become a health concern due to its endocrine disrupting properties (LaFleur & Schug, 2011).…”

Section: Introductionmentioning

confidence: 99%

Effect of Contact Time on the Level of Phthalates in Polyethylene Terephthalate-bottled Water from the Point of Sale

Hazira¹,

Fatimah²,

Selamat³

et al. 2021

JTAS

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The study aimed to evaluate the effect of contact time on the level of phthalates in polyethylene terephthalate (PET)-bottled water commercially available on the market. Different water types (drinking water, mineral water, and sparkling water) in PET bottles and mineral water in glass bottles were collected. Control (before bottling) and freshly produced (0-month) samples were collected at manufacturing sites. In contrast, samples at 6, 12, and 18 months of contact times were collected randomly from hypermarkets and supermarkets in Klang Valley, Malaysia. The samples were analyzed using LC-MS/MS with deuterated DEHP as the internal standard. DEHP, DMP, DEP, DnOP, and BBP were not detected in drinking, mineral, and sparkling water in both PET and glass bottles. However, DBP was detected within the range of 0.68 to 1.11 ng/mL for mineral water and 0.55 to 0.59 ng/ mL for drinking water in PET bottles. All types of phthalates, including DBP, were not detected in the control and 0-month samples. DBP was detected at 0.59 ng/mL at 6 months of contact time and 0.55 ng/mL at 12 months of contact time in PET-bottled drinking water samples; the difference, however, was not significant. It appears that contact time did not significantly affect DBP levels.

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High Throughput Bioassays Using Nanoparticles

Cited by 4 publications

References 73 publications

Surface-Enhanced Raman Scattering Detection and Tracking of Nanoprobes: Enhanced Uptake and Nuclear Targeting in Single Cells

Surface-Enhanced Raman Scattering Detection and Tracking of Nanoprobes: Enhanced Uptake and Nuclear Targeting in Single Cells

Universal Antibody Conjugation to Nanoparticles Using the Fcγ Receptor I (FcγRI): Quantitative Profiling Of Membrane Biomarkers

Effect of Contact Time on the Level of Phthalates in Polyethylene Terephthalate-bottled Water from the Point of Sale

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