High-Throughput Carbohydrate Microarray Technology

Wang, Guoyin; Wang, Ruobing; Shah, Dhaval; Liu, Shaoyi; Wang, Aili; Xu, Xiaoyuan; Liu, Ke; Trummer, Brian J.; Deng, Chao; Cheng, Rong

doi:10.1007/978-1-59745-328-8_14

Cited by 2 publications

(1 citation statement)

References 33 publications

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“…Surface tethered polymers have been used to stabilize colloidal particles and in a wide range of innovative biomedical applications: stealth surfaces resistant to protein adsorption, 5,6 targeted magnetic hyperthermia, 7 targeted drug delivery, 8 genotyping, 9 and the emerging field of glycomics (i.e., carbohydrate microarrays). 10 All involve covalent attachment of some synthetic or biological macromolecule of interest (such as DNA, sugars, proteins, and synthetic polymers) to the surface of substrates such as silicon wafers, nanoparticles, and even micelles.…”

Section: ■ Introductionmentioning

confidence: 99%

Kinetics of Polymer Interfacial Reactions: Polymer Brush Formation by Click Reactions of Alkyne End-Functional Polymers with Azide-Functional Substrates

Zhang

Luo

et al. 2016

Macromolecules

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Attenuated total reflectance infrared spectroscopy (ATR-IR) is used to directly measure the rates of copper-catalyzed 1,3-dipolar cycloaddition reactions between alkyne-terminated polystyrene and poly(n-butyl acrylate) and azide-functional substrates in the good solvent DMF. Four regimes of behavior are observed: initially, the reaction rate is diffusion-controlled scaling with t 1/2; in the crossover regime at the onset of chain overlap, the rate scales with ln(t); the rate then accelerates briefly; and finally, in the terminal or penetration-limited regime, the logarithm of areal density scales linearly with time. Kinetic behavior in the diffusion-limited, crossover, and penetration-limited regimes corresponds well to the predictions of Ligoure and Leibler. The blob model suggests that the acceleration in rate is due to lateral chain contraction during the mushroom to brush transition. A theory is presented to predict that the areal density at saturation should scale as Σsaturation ∼ M w –1.2 for good solvents, and experimentally we find M w –0.93±0.04 scaling.

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Section: ■ Introductionmentioning

confidence: 99%

Kinetics of Polymer Interfacial Reactions: Polymer Brush Formation by Click Reactions of Alkyne End-Functional Polymers with Azide-Functional Substrates

Zhang

Luo

et al. 2016

Macromolecules

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Multiplexed Antibody Arrays for The Discovery and Validation of Glycosylated Protein Biomarkers

Nelson¹

2009

Bioanalysis

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Protein glycosylation, the enzymatic linkage of mono- and poly-saccharides to proteins, is a critical determinant of protein function; however, there is a lack of tools for studying the glycosylation of specific proteins in complex samples. A new type of antibody-lectin sandwich assay enables the measurement of the glycosylation of specific proteins that have been captured from complex samples using antibody arrays combined with lectin-based detection probes. Antibody-lectin sandwich arrays have the potential to expand our understanding of the role of glycans and protein glycosylation in disease and to identify and investigate new biomarkers for early detection, disease prognosis and therapeutic response prediction. While antibody-lectin sandwich arrays yield less-detailed structural information regarding protein glycosylation than other available methods, they do provide a simple and reproducible method for investigating changes in protein abundance and glycosylation of multiple proteins and can be easily applied to large or small sample sets. By profiling protein and glycan variations, new disease-associated glycan alterations can be identified and validated for use as biomarkers.

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High-Throughput Carbohydrate Microarray Technology

Cited by 2 publications

References 33 publications

Kinetics of Polymer Interfacial Reactions: Polymer Brush Formation by Click Reactions of Alkyne End-Functional Polymers with Azide-Functional Substrates

Kinetics of Polymer Interfacial Reactions: Polymer Brush Formation by Click Reactions of Alkyne End-Functional Polymers with Azide-Functional Substrates

Multiplexed Antibody Arrays for The Discovery and Validation of Glycosylated Protein Biomarkers

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