2019
DOI: 10.1063/1.5128797
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High-throughput continuous dielectrophoretic separation of neural stem cells

Abstract: We created an integrated microfluidic cell separation system that incorporates hydrophoresis and dielectrophoresis modules to facilitate high-throughput continuous cell separation. The hydrophoresis module consists of a serpentine channel with ridges and trenches to generate a diverging fluid flow that focuses cells into two streams along the channel edges. The dielectrophoresis module is composed of a chevron-shaped electrode array. Separation in the dielectrophoresis module is driven by inherent cell electro… Show more

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Cited by 48 publications
(51 citation statements)
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“…Electrode-based DEP has been routinely used to manipulate particles [ 14 , 32 , 59 , 60 ] and cells [ 9 , 57 , 61 ]. Electrodes in eDEP are generally made of electrically conductive materials, such as silver, brass, and gold.…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…Electrode-based DEP has been routinely used to manipulate particles [ 14 , 32 , 59 , 60 ] and cells [ 9 , 57 , 61 ]. Electrodes in eDEP are generally made of electrically conductive materials, such as silver, brass, and gold.…”
Section: Discussionmentioning
confidence: 99%
“…Due to the application of DEP on neutral particles, it became apparent that the same electric fields can be employed to manipulate biological cells. The findings by Pohl paved the way for current research on inhomogeneous electric fields in biology [ 1 , 6 , 7 , 8 , 9 ].…”
Section: Introductionmentioning
confidence: 99%
“…The cell patterning capabilities of EIS combined with DEP can be employed to build complex 3D microenvironments that represent in vivo cancer conditions. Another aspect of DEP is its implementation to continuously sort a variety of cells [ 81 , 101 , 102 , 103 , 104 ], thus an exciting possibility is to sort CSCs from non-CSCs prior to impedance monitoring. These techniques can also be used for drug optimization and monitoring as well as to understand the molecular and environmental driving forces involved in plasticity.…”
Section: Future Trends In Monitoring Cancer Cell Dynamicsmentioning
confidence: 99%
“…Our newly developed DEP cell-sorting system does not require antibodies, fluorescence dyes, magnetic beads, or expensive equipment. In addition, the process of our DEP cell-sorting system was simple when compared to other DEP cell-sorting systems [41][42][43][44][45][46], with no complicated processes and with a high extensibility. Furthermore, our DEP cell-sorting system does not require a numerical analysis of the fluid-flow in the DEP chamber.…”
Section: Purification Efficacy Of Dep Cell-sorting Devicementioning
confidence: 99%