High throughput culture and gametogenesis induction of Laminaria japonica gametophyte clones

Zhang, Quan Sheng; Qu, Shan Cun; Cong, Yi Zhou; Luo, Shi Ju; Tang, Xue Xi

doi:10.1007/s10811-007-9220-5

Cited by 39 publications

(39 citation statements)

References 19 publications

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“…Apparently, this so-called free-living technique crucially depends on the propagation of male and female gametophytes under controlled conditions. Zhang et al (2008) reported that temperature (5, 10, and 15°C) had no significant effects on the growth of gametophyte clones which were cultured in 20-L flasks for 15 days and continuously bubbled with filtered air. Li (2003) stated that enhancing temperature could increase the cell number of female and male gametophyte clones.…”

Section: Discussionmentioning

confidence: 99%

Growth performance of unialgal gametophytes of the brown alga Saccharina japonica in mass culture conditions

2015

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For the purpose of mass culture of Saccharina japonica unialgal gametophytes originated from spores in laboratory conditions to breed sporelings, a series of experiments were designed to evaluate vegetative growth performance of male and female gametophytes. After 8-day culture, female gametophytes exhibited the higher relative growth rates (RGRs) around 8.29-9.27 % day −1 than male ones (5.30-6.10 % day −1 ). Both male and female gametophytes had the higher RGRs in NO 3 − -supplemented medium than that in NH 4 + medium under equal nitrogen concentration. The RGRs of the female and male could reach the peak levels of 8.96 and 5.91 % day −1 in NO 3 − group and 6.60 and 3.89 % day −1 in NH 4 + group, respectively. Elevation of temperature from 5 to 15°C significantly promoted the vegetative growth of both in the medium enriched with 450 μM of NO 3 − or NH 4 + . At higher temperature (25°C), the growth rates of both were retarded greatly with the female being more sensitive to the exposure to 450 μM of NH 4 + , and the photosynthesis activity was partially damaged as reflected by the decrease of the optimal chlorophyll fluroscence quantum yield (F v /F m ) from 0.657±0.023 to 0.401±0.053.

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Section: Discussionmentioning

confidence: 99%

Growth performance of unialgal gametophytes of the brown alga Saccharina japonica in mass culture conditions

2015

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“…Male and female gametophyte fragments were mixed and cultured in suspension in 20-L bottles at 10°C and an irradiance of 60 µmol photons m −2 s −1 in a 10:14 h light-dark cycle (Zhang et al 2008). A sex ratio (female:male) of 3:1 based on the biomass of gametophytes was used for induction of gametogenesis.…”

Section: Preparation Of Fertile Gametophytesmentioning

confidence: 99%

“…During the expansion and induction of vegetative gametophytes, any operations are strictly disinfected in order to avoid contamination (Zhang et al 2008); no epiphytes were detectable in the collectors during the early culture stage, thus avoiding the need for collectors to be spray-washed. The present results show that most sporophytes grew on the palm ropes via rhizoids once they had grown up to 8-16 rows cells.…”

Section: Species Ofmentioning

confidence: 99%

“…A mass of fertile gametophytes is a prerequisite for largescale seedling production using vegetative gametophytes (Wu et al 1994), and the relevant techniques required to produce these have been recently reported (Zhang et al 2008). The aims of this study were to investigate the key techniques pertaining to the attachment of Laminaria gametophytes and culture of seedlings under commercial production conditions.…”

Section: Introductionmentioning

confidence: 99%

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Introduction of a seedling production method using vegetative gametophytes to the commercial farming of Laminaria in China

Zhang

Qu³

et al. 2008

J Appl Phycol

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Laminaria is the main seaweed species under commercial cultivation in China, which utilizes 80 x 10 9 artificially cultured seedlings each year. This paper reports the large-scale application of seedling production using vegetative gametophytes in Laminaria cultivation. Key techniques, such as the attachment of gametophytes to the collectors and tank culture of seedlings in the hatchery, are examined. The problems of detachment and irregular dispersion of seedlings were also investigated. An efficient and practical seedling production technology was developed, which consists of seeding gametophytes on collectors, control of seawater flow in tanks, washing of collectors and regulation of ambient light, seawater temperature and nutrition supplement. After 2 months of culture, seedlings grown on woven palm rope collectors 0.5 cm in diameter measured up to the national technical standard of China (i.e., > 20 individuals cm −1 at a length of > 1.5 cm, and > 100 individuals cm −1 at a length of 0.2 cm-1.5 cm, desolation rate < 1% and detachment rate < 5%). Compared with spore-derived seedlings, no significant differences in sporophyte recruitment and dispersion pattern were observed. Large-scale production of seedlings for commercial cultivation of 4,000 ha from 2001 to 2006 was conducted successfully.

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“…One of the advantageous features of kelps is the capacity of their haploid male and female gametophytes to be multiplied vegetatively through mitosis (Perèz et al 1992;Pang et al 1997;Zhang et al 2008). Though mostly annual in nature, the gametophytes can be grown under strictly controlled conditions for a number of years and be crossed to produce identical sporophytes (Shan and Pang 2009;Xu et al 2009).…”

Section: Introductionmentioning

confidence: 99%

Intraspecific crossing of Saccharina japonica using distantly related unialgal gametophytes benefits kelp farming by improving blade quality and productivity at Sanggou Bay, China

Zhao¹,

Pang

Liu

et al. 2015

J Appl Phycol

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When cultivating the kelp, Saccharina japonica, in China, one of the causes leading to degeneration in blade quality and productivity has been thought to be inbreeding caused by the use of limited numbers of sorus-bearing individuals that are continuously used over generations to produce large numbers of offspring. One of the effective solutions is to use intraspecific hybrid vigor to produce new cultivars or to recreate the original agronomic features of the older cultivars. This study produced a sporophytic B013 (cultivar code) by crossing a pair of distantly related unialgal male and female gametophyte clones. The male was isolated from an individual collected from a farmed population at Rongcheng, China, while the female came from a Korean wild individual sporophyte. In comparison with four controls, the B013 sporophytic offspring demonstrated excellent blade quality (entirely dark green in the dried product), high alginate (29.7-32.9 %) and iodine (0.29-0.31 mg g −1 ) contents in the dried product, the lowest fresh weight/sun-dried weight (FW/DW) ratio and the smallest coefficients of variation for total length (7.4 %), width (6.9 %), and biomass output (14.6 %). In addition, this cultivar remained in a vegetative state from June to August, greatly reducing the biomass loss during the hot summer season. In comparison with seeding zoospores for seedling production, seeding a selected pair of unialgal gametophytes had apparent advantages in effectively preventing inbreeding depression, which gave rise to a uniformed product and provided new cultivars for large-scale farming.

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High throughput culture and gametogenesis induction of Laminaria japonica gametophyte clones

Cited by 39 publications

References 19 publications

Growth performance of unialgal gametophytes of the brown alga Saccharina japonica in mass culture conditions

Growth performance of unialgal gametophytes of the brown alga Saccharina japonica in mass culture conditions

Introduction of a seedling production method using vegetative gametophytes to the commercial farming of Laminaria in China

Intraspecific crossing of Saccharina japonica using distantly related unialgal gametophytes benefits kelp farming by improving blade quality and productivity at Sanggou Bay, China

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