High throughput deep proteomic analysis of seminal plasma from stallions with contrasting semen quality

Kumaresan, A.; Paul, Nilendu; Sinha, Manish Kumar; King, John Peter Ebenezer Samuel; Elango, K.; Sharma, Ankur; Raval, Kathan; Legha, Ram Avtar; Pal, Yash

doi:10.1080/19396368.2022.2057257

Cited by 8 publications

(2 citation statements)

References 100 publications

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“…MS1 spectra were acquired in the Orbitrap at a resolution of 70 k. Dynamic exclusion was employed for 10 s excluding all charge states for a given precursor. MS2 spectra were acquired at rate of 17,500 resolutions 60 . All the samples were processed and RAW files generated were analyzed with Proteome Discoverer (v2.4) against the Uniprot Bovine reference proteome database.…”

Section: Methodsmentioning

confidence: 99%

Establishment of a repertoire of fertility associated sperm proteins and their differential abundance in buffalo bulls (Bubalus bubalis) with contrasting fertility

Kumaresan

Sinha²,

Paul³

et al. 2023

Sci Rep

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Sperm harbours a wide range of proteins regulating their functions and fertility. In the present study, we made an effort to characterize and quantify the proteome of buffalo bull spermatozoa, and to identify fertility associated sperm proteins through comparative proteomics. Using high-throughput mass spectrometry platform, we identified 1305 proteins from buffalo spermatozoa and found that these proteins were mostly enriched in glycolytic process, mitochondrial respiratory chain, tricarboxylic acid cycle, protein folding, spermatogenesis, sperm motility and sperm binding to zona pellucida (p < 7.74E−08) besides metabolic (p = 4.42E−31) and reactive oxygen species (p = 1.81E−30) pathways. Differential proteomic analysis revealed that 844 proteins were commonly expressed in spermatozoa from both the groups while 77 and 52 proteins were exclusively expressed in high- and low-fertile bulls, respectively. In low-fertile bulls, 75 proteins were significantly (p < 0.05) upregulated and 176 proteins were significantly (p < 0.05) downregulated; these proteins were highly enriched in mitochondrial respiratory chain complex I assembly (p = 2.63E−07) and flagellated sperm motility (p = 7.02E−05) processes besides oxidative phosphorylation pathway (p = 6.61E−15). The down regulated proteins in low-fertile bulls were involved in sperm motility, metabolism, sperm-egg recognition and fertilization. These variations in the sperm proteome could be used as potential markers for the selection of buffalo bulls for fertility.

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Section: Methodsmentioning

confidence: 99%

Establishment of a repertoire of fertility associated sperm proteins and their differential abundance in buffalo bulls (Bubalus bubalis) with contrasting fertility

Kumaresan

Sinha²,

Paul³

et al. 2023

Sci Rep

Self Cite

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“…As far as the authors know, the first comprehensive study of the proteome of the stallion spermatozoa was conducted by Australian researchers at the University of Newcastle [2]. Since this first study, proteomic and phospho-proteomic approaches have been introduced to the study of stallion sperm biology [3][4][5][6][7][8][9][10][11]. This review aims to provide an updated overview of the major findings and advances in the comprehension of the stallion spermatozoa obtained thanks to the introduction of proteomics to the study of stallion sperm biology, and the impact of these findings on sperm biotechnology in equines.…”

mentioning

confidence: 99%

Proteomics is advancing the understanding of stallion sperm biology

Peña,

Martín‐Cano,

Becerro‐Rey

et al. 2024

Proteomics

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The mammalian ejaculate is very well suited to proteomics studies. As such, research concerning sperm proteomics is offering a huge amount of new information on the biology of spermatozoa. Among domestic animals, horses represent a species of special interest, in which reproductive technologies and a sizeable market of genetic material have grown exponentially in the last decade. Studies using proteomic approaches have been conducted in recent years, showing that proteomics is a potent tool to dig into the biology of the stallion spermatozoa. The aim of this review is to present an overview of the research conducted, and how these studies have improved our knowledge of stallion sperm biology. The main outcomes of the research conducted so far have been an improved knowledge of metabolism, and its importance in sperm functions, the impact of different technologies on the sperm proteome, and the identification of potential biomarkers. Moreover, proteomics of seminal plasma and phosphoproteomics are identified as areas of major interest.

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An updated review on the application of proteomics to explore sperm cryoinjury mechanisms in livestock animals

Larbi,

Li,

Quan

2024

Animal Reproduction Science

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High throughput deep proteomic analysis of seminal plasma from stallions with contrasting semen quality

Cited by 8 publications

References 100 publications

Establishment of a repertoire of fertility associated sperm proteins and their differential abundance in buffalo bulls (Bubalus bubalis) with contrasting fertility

Establishment of a repertoire of fertility associated sperm proteins and their differential abundance in buffalo bulls (Bubalus bubalis) with contrasting fertility

Proteomics is advancing the understanding of stallion sperm biology

An updated review on the application of proteomics to explore sperm cryoinjury mechanisms in livestock animals

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