High-Throughput Detection of Multiple Contaminants Based on Portable Photoelectrochromic Sensor Chip

Abstract

With the increasing concerns about the environment and food safety, it is necessary to develop portable, low-cost, and highthroughput biosensors for the simultaneous detection of multiple contaminates. However, traditional photoelectrochemical (PEC) biosensors lack the ability of multiplexed assays due to the inherent mechanism limitation. Also, specialized instruments are necessary for most PEC biosensors. In this work, a portable high-throughput sensor chip has been successfully developed. By introducing electrochromic materials, the detection is based on color change instead of electric signals, which reduces the limitation of instruments. This designed sensor chip is composed of three parallel sensing channels fabricated by laser etching. Each channel is modified with TiO 2 /3D-g-C 3 N 4 composites with excellent PEC activity and electrochromic material Prussian blue (PB). Under light illumination, photoinduced electrons generated by TiO 2 /3D-g-C 3 N 4 are injected into PB, and blue PB is reduced to colorless Prussian white. Three organic contaminates, ochratoxin A, lincomycin, and edifenphos, can be simultaneously detected because the binding of these molecules with aptamers affects the electron transfer and the corresponding color changes. This portable and high-throughput sensor chip provides a convenient choice for multiplexed assays with good sensitivity and accuracy.

Biomimic Nanozymes with Tunable Peroxidase-like Activity Based on the Confinement Effect of Metal–Organic Frameworks (MOFs) for Biosensing

“…Paper-and Gel-Based Sensors for H 2 O 2 and Glucose Detection. Developing a visual, rapid, and real-time strategy for the monitoring of targets has always been an important issue in many fields, such as environment contaminations, 34,35 food safety management, 36 and point-of-care diagnostic tests. 37 Herein, utilizing Hemin@BSA@ZIF-8, a portable and sensitive paper-based colorimetric sensor integrated with a smartphone was designed for the naked-eye and quantitative analysis of H 2 O 2 and glucose.…”

A ZnIn2S4@ReS2/AgInS2-based photoelectrochemical aptasensor for the ultrasensitive detection of kanamycin

“…7 Besides that, the separated excitation light source and output signal make PEC sensing have a low background signal, which ensures the superior sensitivity 8,9 Taking into account these advantages, PEC sensing has received extensive attention in various kinds of target detection such as proteins, cancer markers, glucose, organic pesticides and so on. 10,11 As is known, the photoelectric conversion efficiency of a photoactive material is a critical factor for the sensitivity of the sensor. The exploration of materials with high photoelectroactivity continues to be a great impetus to design and develop PEC sensors.…”

“…7 Besides that, the separated excitation light source and output signal make PEC sensing have a low background signal, which ensures the superior sensitivity 8,9 Taking into account these advantages, PEC sensing has received extensive attention in various kinds of target detection such as proteins, cancer markers, glucose, organic pesticides and so on. 10,11…”

Dual-Signaling Photoelectrochemical Biosensor Based on Biocatalysis-Induced Vulcanization of Bi2MoO6 Nanosheets

“…By integrating the two types of relatively independent signal transduction modes, coresponse bioassays can greatly reduce the influence of uncontrollable accidental errors or background signals during the operating process, thereby improving the accuracy level of the test . Of particular note is that colorimetric methods are promising tools, which can be applied for quantitative or semiquantitative assays and unlocking the restriction of using sophisticated and expensive instruments due to their advantages of low cost, visualization, and simplified analytical procedures. , Hence, compared to other dual-signaling strategies, bioassays coupled with PEC and CL have the potential to replace traditional equipment for the construction of biosensors, which are portable and show rapid response and high confidence levels to a certain extent. , In this regard, developing an innovative biosensing platform based on PEC analysis and visual detection by naked eyes is of great importance for the development of portable and miniaturized biosensing devices.…”