High-Throughput Experimentation Using Cell-Free Protein Synthesis Systems

Meyer, Conary; Zhou, Chuqing; Fang, Zecong; Longo, Marjorie L.; Pan, Tingrui; Tan, Cheemeng

doi:10.1007/978-1-0716-1998-8_7

Cited by 1 publication

(1 citation statement)

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“…The cell-free transcription-translation system is derived from E. coli extract and is capable of synthesizing approximately 1 mg/mL of proteins, including those up to 150 kDa in size, in less than 3 h. 70 Utilizing the open nature of cell-free protein synthesis, they have devised a rapid method for prototyping nanovesicles using a custom droplet printing robot. 71 The droplet printer uses a new impact-printing-based methodology to generate droplet arrays in nanoliter scale in 384-well plates. This method is highly adaptable, allowing them to vary lipid types, chemical environments, and chaperone proteins.…”

Section: Introductionmentioning

confidence: 99%

Cracking the Code: Enhancing Molecular Tools for Progress in Nanobiotechnology

Avila,

Rebolledo,

Skelly

et al. 2024

ACS Appl. Bio Mater.

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Nature continually refines its processes for optimal efficiency, especially within biological systems. This article explores the collaborative efforts of researchers worldwide, aiming to mimic nature's efficiency by developing smarter and more effective nanoscale technologies and biomaterials. Recent advancements highlight progress and prospects in leveraging engineered nucleic acids and proteins for specific tasks, drawing inspiration from natural functions. The focus is developing improved methods for characterizing, understanding, and reprogramming these materials to perform user-defined functions, including personalized therapeutics, targeted drug delivery approaches, engineered scaffolds, and reconfigurable nanodevices. Contributions from academia, government agencies, biotech, and medical settings offer diverse perspectives, promising a comprehensive approach to broad nanobiotechnology objectives. Encompassing topics from mRNA vaccine design to programmable protein-based nanocomputing agents, this work provides insightful perspectives on the trajectory of nanobiotechnology toward a future of enhanced biomimicry and technological innovation.

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Section: Introductionmentioning

confidence: 99%