High-Throughput HPLC-MS/MS Method for Quantification of Ibuprofen Enantiomers in Human Plasma: Focus on Investigation of Metabolite Interference

Nakov, Natalija; Bogdanovska, Liljana; Acevska, Jelena; Tonic-Ribarska, Jasmina; Petkovska, Rumenka; Dimitrovska, Aneta; Kasabova, Lilia; Svinarov, Dobrin

doi:10.1093/chromsci/bmw166

Cited by 3 publications

(3 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The quantification of (R)-IBP and (S)-IBP in human plasma subsequent to the oral administration of 400 mg racemic IBP was conducted. 22 Following oral absorption, the levels of the enantiomers surged swiftly, attaining peak plasma concentrations within 120 min. The peak plasma concentration (mean ± SD, n = 5) for (R)-IBP and (S)-IBP were measured at 14.54 ± 3.18 mg/L and 13.37 ± 2.28 mg/L, respectively.…”

Section: Stereoselective Pharmacokinetics Of (R)-and (S)-ibpmentioning

confidence: 99%

See 1 more Smart Citation

Enantioselective separation and determination of ibuprofen: Stereoselective pharmacokinetics, pharmacodynamics and analytical methods

Vashistha,

Kumar,

Yadav

et al. 2024

Chirality

View full text Add to dashboard Cite

Ibuprofen (IBP), the 29th most prescribed drug in the United States in 2019, is a widely used nonsteroidal anti‐inflammatory drug (NSAID) comprising two enantiomers, (R)‐IBP and (S)‐IBP, collectively known as (RS)‐IBP. This critical review examines analytical techniques for the enantioselective separation and determination of IBP enantiomers, crucial for pharmaceutical and clinical applications. The review focuses on state‐of‐the‐art methods, including chromatographic techniques including high‐performance liquid chromatography, gas chromatography, liquid chromatography–tandem mass spectrometry, and some other techniques. This review addresses pharmacokinetics, pharmacology, and side effects of each enantiomer, ensuring safe drug usage. By consolidating diverse analytical methods and their applicability in different matrices, this review serves as a valuable resource for researchers, analysts, and practitioners in pharmaceutical analysis, pharmacology, and clinical studies.

show abstract

Section: Stereoselective Pharmacokinetics Of (R)-and (S)-ibpmentioning

confidence: 99%

“…The quantification of ( R )‐IBP and ( S )‐IBP in human plasma subsequent to the oral administration of 400 mg racemic IBP was conducted 22 . Following oral absorption, the levels of the enantiomers surged swiftly, attaining peak plasma concentrations within 120 min.…”

Section: Stereochemical Aspects Of Ibpmentioning

confidence: 99%

Enantioselective separation and determination of ibuprofen: Stereoselective pharmacokinetics, pharmacodynamics and analytical methods

Vashistha,

Kumar,

Yadav

et al. 2024

Chirality

View full text Add to dashboard Cite

show abstract

“…Concentrations of R-(-)-IBP and S-(+)-IBP were determined using validated high-performance liquid chromatography coupled with a tandem mass spectrometry detection (HPLC MS/MS) chiral method according to Nakov et al (23). Estimation of the PK parameters was performed from the plasma concentration-time dependent data by a standard non-compartmental method using Pharsight ® Knowledgebase ServerTM and Phoenix ® WinNonlin ® .…”

Section: Pharmacokinetic Analysismentioning

confidence: 99%

AKR1D1*36 C>T (rs1872930) allelic variant is associated with variability of the CYP2C9 genotype predicted pharmacokinetics of ibuprofen enantiomers – a pilot study in healthy volunteers

et al. 2019

View full text Add to dashboard Cite

The relative contribution of CYP2C9 allelic variants to the pharmacokinetics (PK) of ibuprofen (IBP) enantiomers has been studied extensively, but the potential clinical benefit of pharmacogenetically guided IBP treatment is not evident yet. The role of AKR1D1*36C>T (rs 1872930) allelic variant in interindividual variability of CYP450 mediated drug metabolism was recently elucidated. A total of 27 healthy male subjects, volunteers in IBP single-dose two-way cross-over bioequivalence studies were genotyped for CYP2C9*2, CYP2C9*3 and AKR1D1*36 polymorphisms. The correlation between CYP2C9 and AKR1D1 genetic profile and the PK parameters for S-(+) and R-(−)-IBP was evaluated. Remarkable changes in the PK values pointing to reduced CYP2C9 enzyme activity were detected only in the CYP2C9*2 allelic variant carriers. Statistically significant association between the AKR1D1*36 allele and the increased IBP metabolism (low AUC0-t and 0–∞, high Cltot and short tmax values for both enantiomers) was observed in subjects carrying the CYP2C9 *1/*3 or CYP2C9*1/*1 genotype. The clinical value of concomitant CYP2C9 and AKR1D1 genotyping has to be further verified.

show abstract

Analysis of Different Methods of Extracting NSAIDs in Biological Fluid Samples for LC-MS/MS Assays: Scoping Review

et al. 2022

View full text Add to dashboard Cite

The aim of this study was to carry out a systematic investigation and analysis of different drug extraction methods, specifically non-steroidal anti-inflammatory drugs in biological fluid samples, for Liquid Chromatography in Mass Spectrometry assays (LC-MS/MS). A search was carried out in the main databases between 1999 and 2021, following the Preferred Reporting Items for Systematic reviews and Meta-Analyses extension for Scoping Reviews (PRISMA-ScR) checklist. Data were obtained through PubMed, Lilacs, Embase, Scopus, and Web of Science databases using the Boolean operators AND and OR. Studies were pre-selected by title and abstract by two independent reviewers. The selected texts were read in full, and only those that were complete and compatible with the inclusion and exclusion criteria were eligible for this research. A total of 248 references were obtained in the databases. After removing the duplicates and analyzing the titles and abstracts, 79 references were evaluated and passed to the next phase, which comprised the complete reading of the article. A total of 39 publications were eligible for this study. In 52% of the studies, the authors used the liquid–liquid extraction method (LLE), while in 41%, the solid-phase extraction method (SPE) was used. A total of 5% used microextraction methods and 2% used less-conventional techniques. The literature on the main methods used, the LLE and SPE methods, is extensive and consolidated; however, we found other studies that reported modifications of these traditional techniques, which were equally validated for use in LC-MS/MS. From this review, it is concluded that the diversity of techniques, reliability, and practical information about each analytical method used in this study can be adapted to advances in LC-MS/MS techniques; however, more ecological, economic, and sustainable approaches should be explored in the future.

show abstract

High-Throughput HPLC-MS/MS Method for Quantification of Ibuprofen Enantiomers in Human Plasma: Focus on Investigation of Metabolite Interference

Cited by 3 publications

References 20 publications

Enantioselective separation and determination of ibuprofen: Stereoselective pharmacokinetics, pharmacodynamics and analytical methods

Enantioselective separation and determination of ibuprofen: Stereoselective pharmacokinetics, pharmacodynamics and analytical methods

AKR1D1*36 C>T (rs1872930) allelic variant is associated with variability of the CYP2C9 genotype predicted pharmacokinetics of ibuprofen enantiomers – a pilot study in healthy volunteers

Analysis of Different Methods of Extracting NSAIDs in Biological Fluid Samples for LC-MS/MS Assays: Scoping Review

Contact Info

Product

Resources

About