High-Throughput Identification of Organic Compounds from Polygoni Multiflori Radix Praeparata (Zhiheshouwu) by UHPLC-Q-Exactive Orbitrap-MS

Abstract

Polygoni Multiflori Radix Praeparata (PMRP), as the processed product of tuberous roots of Polygonum multiflorum Thunb., is one of the most famous traditional Chinese medicines, with a long history. However, in recent years, liver adverse reactions linked to PMRP have been frequently reported. Our work attempted to investigate the chemical constituents of PMRP for clinical research and safe medication. In this study, an effective and rapid method was established to separate and characterize the constituents in PMRP by combining ultra-high performance liquid chromatography with hybrid quadrupole-orbitrap mass spectrometry (UHPLC-Q-Exactive Orbitrap-MS). Based on the accurate mass measurements for molecular and characteristic fragment ions, a total of 103 compounds, including 24 anthraquinones, 21 stilbenes, 15 phenolic acids, 14 flavones, and 29 other compounds were identified or tentatively characterized. Forty-eight compounds were tentatively characterized from PMRP for the first time, and their fragmentation behaviors were summarized. There were 101 components in PMRP ethanol extract (PMRPE) and 91 components in PMRP water extract (PMRPW). Simultaneously, the peak areas of several potential xenobiotic components were compared in the detection, which showed that PMRPE has a higher content of anthraquinones and stilbenes. The obtained results can be used in pharmacological and toxicological research and provided useful information for further in vitro and in vivo studies.

Molecular identification and functional characterization of two glycosyltransferases genes from Fallopia multiflora

“…As a perennial herb in the Polygonaceae family, Fallopia multiflora is generally used in traditional Chinese medicine and contains various major active components including stilbenes, anthraquinones (AQs), flavonoids, and polysaccharides ( Bi et al., 2020 ; Wang et al., 2021 ). The herb has a wide range of effects in clinical and therapeutic care, and previous research on its active components has focused on 2, 3, 5, 4’- tetrahydroxy stilbene-2- O-β- d -glucoside (THSG) and AQ glucosides.…”

Elucidation of active ingredients and mechanism of action of hawthorn in the prevention and treatment of atherosclerosis

“…The ion at m/z 127.03893 was produced by losing COO and H 2 O from precursor ion. Compared with the reference literature, compound 1 was identified as quinic acid (Wang et al, 2021).…”

Cinnamomi ramulus inhibits cancer cells growth by inducing G2/M arrest

“…Analysis of CR chemical composition was performed using UPLC-MS/MS. UPLC conditions: Chromatographic separation was achieved using Vanquish Flex UHPLC (Thermo Fisher Scientific, Bremen, Germany) (Wang et al, 2021), and the chromatographic column used was ACQUITY UPLC T3 column (100 mm*2.1 mm, 1.7 µm, Waters, Milford, United States). The mobile phase consisted of solvent (A) 0.1% formic acid and (B) Acetonitrile.…”

“…The gradient elution was as follows: 0-0.8 min, 2% B; 0.8-2.8 min, 2%-70% B; 2.8-5.6 min, 70%-90% B; 5.6-6.4 min, 90%-100% B; 6.4-8 min, 100% B; 8-8.1 min, 100%-2% B; 8.1-10 min, 2% B. MS conditions: Mass spectra were obtained on Q-Exactive (Thermo Scientific). Both positive and negative ion modes were performed on Q-Exactive (Wang et al, 2021).…”