High-throughput investigation of osteoblast response to polymer crystallinity: influence of nanometer-scale roughness on proliferation

Washburn, Newell R.; Yamada, Katsushi; Simon, Carl G.; Kennedy, Scott B.; Amis, Eric J.

doi:10.1016/j.biomaterials.2003.08.043

Cited by 295 publications

(261 citation statements)

References 24 publications

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“…At relatively high roughness (submicrometer down to ~100 nm), it is clear that substrate topography influences cell response, the cells responding to roughness by a higher cell thickness and a delayed apparition of the focal contacts [5,35] . At the nanometer scale, Washburn et al [36] showed that the rate of proliferation on the smooth regions of a poly(Llactic acid) film (below 1 nm) is much greater than on the rough regions (around 12 nm). The observed variations in proliferation were however weak in the 3-7nm range.…”

Section: Relative Importance Of Film Stiffness Roughness and Wettabimentioning

confidence: 99%

Polyelectrolyte Multilayer Films of Controlled Stiffness Modulate Myoblast Cell Differentiation

Ren

Crouzier

Roy

et al. 2008

Adv Funct Materials

246

263

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Beside chemical properties and topographical features, mechanical properties of gels have been recently demonstrated to play an important role in various cellular processes, including cell attachment, proliferation, and differentiation. In this work, we used multilayer films made of poly (L-lysine)/Hyaluronan (PLL/HA) of controlled stiffness to investigate the effects of mechanical properties of thin films on skeletal muscle cells (C2C12 cells) differentiation. Prior to differentiation, cells need to adhere and proliferate in growth medium. Stiff films (E 0 > 320 kPa) promoted formation of focal adhesions and organization of the cytoskeleton as well as an enhanced proliferation, whereas soft films were not favorable for cell anchoring, spreading or proliferation. Then C2C12 cells were switched to a low serum containing medium to induce cell differentiation, which was also greatly dependent on film stiffness. Although myogenin and troponin T expressions were only moderately affected by film stiffness, the morphology of the myotubes exhibited striking stiffness-dependent differences. Soft films allowed differentiation only for few days and the myotubes were very short and thick. Cell clumping followed by aggregates detachment could be observed after ~2 to 4 days. On stiffer films, significantly more elongated and thinner myotubes were observed for up to ~ 2 weeks. Myotube striation was also observed but only for the stiffer films. These results demonstrate that film stiffness modulates deeply adhesion, proliferation and differentiation, each of these processes having its own stiffness requirement.

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Section: Relative Importance Of Film Stiffness Roughness and Wettabimentioning

confidence: 99%

Polyelectrolyte Multilayer Films of Controlled Stiffness Modulate Myoblast Cell Differentiation

Ren

Crouzier

Roy

et al. 2008

Adv Funct Materials

246

263

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“…Washburn et al 101 used a temperature gradient to induce varying crystallinity and thus a roughness gradient on the nanometre length-scale. They created a thin film of poly(L-lactic acid) (PLLA) on a silanised silicon wafer, which was annealed on a temperature gradient ranging from 44 to 100 C. The RMS values achieved with this method ranged from 0.54 to 13 nm.…”

Section: 24mentioning

confidence: 99%

“…Whereas proliferation increased towards a higher surface roughness on the micrometre scale, 117 the opposite behaviour was found for nanofeature gradients. 101,118 In order to adhere to surfaces, cells need to form focal contacts. The spacing between particles is in the nanometre range, meaning much smaller than the size of the cells.…”

mentioning

confidence: 99%

Surface-chemical and -morphological gradients

2008

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Surface gradients of chemistry or morphology represent powerful tools for the high-throughput investigation of interfacial phenomena in the areas of physics, chemistry, materials science and biology. A wide variety of methods for the fabrication of such gradients has been developed in recent years, relying on principles ranging from diffusion to time-dependent irradiation in order to achieve a gradual change of a particular parameter across a surface. In this review we have endeavoured to cover the principal fabrication approaches for surface-chemical and surface-morphological gradients that have been described in the literature, and to provide examples of their applications in a variety of different fields.

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“…Although the cellular response in different surfaces is largely researched, the studies are limited by the use of the terms "smooth or rough", that is, surfaces with roughness values that little varies among them. Additionally, the substrates are produced by different techniques, creating different types of surfaces [28].…”

Section: Seven Daysmentioning

confidence: 99%

Stem cells behaviour on titanium surface modificated by plasma

Sá¹,

Silva²,

Rocha³

et al. 2012

BDS

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Over the last decade, a considerable development of new technologies to the modification of dental implants has been observed contributing to reduce the healing process and their use in areas with low bone density. Among the new techniques, plasma nitriding has showed excellent results. In this study, a superficial modification of commercial pure titanium (Degree II), by using two different plasma treatments (planar and hollow cathode nitriding) was accomplished aiming at an optimization of the surface for biomedical applications. An evaluation of the chemical composition in all samples was carried out, in addition to a study of their roughness and texture. Then, stem cells were deposited onto these surfaces and a comparison among their properties and their biological behavior was accomplished. The results showed that the nitriding techniques produced significant changes in the superficial texture of the Ti samples. The roughness test presented better results in samples nitrided by hollow cathode technique. Statistically significant differences were found in the cell proliferation of samples nitrided by hollow cathode nitriding when compared to samples without treatment. The used techniques were, therefore, effective and directly influenced the characteristics of the titanium surface and consequently, the stem cell behavior.

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High-throughput investigation of osteoblast response to polymer crystallinity: influence of nanometer-scale roughness on proliferation

Cited by 295 publications

References 24 publications

Polyelectrolyte Multilayer Films of Controlled Stiffness Modulate Myoblast Cell Differentiation

Polyelectrolyte Multilayer Films of Controlled Stiffness Modulate Myoblast Cell Differentiation

Surface-chemical and -morphological gradients

Stem cells behaviour on titanium surface modificated by plasma

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