High-throughput laser printing of cells and biomaterials for tissue engineering

Guillemot, Fabien; Souquet, Agnès; Catros, Sylvain; Guillotin, Bertrand; Lopez, John; Fauçon, Marc; Pippenger, Benjamin E.; Bareille, Reine; Rémy, Murielle; Bellance, Séverine; Chabassier, Patrick; Fricain, Jean-Christophe; Amédée, Joëlle

doi:10.1016/j.actbio.2009.09.029

Cited by 417 publications

(253 citation statements)

References 30 publications

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“…Pour pallier ce problème, les matrices étaient imprimées sans cellules et ensemencées secondairement avec le LAB. Cette technique permet d'imprimer des solutions de 5-10×10 7 cellules/mL [12]. Le laser a d'autres avantages comme l'augmentation de la densité cellulaire (plusieurs passages), la combinaison de différents types cellulaires et l'organisation spatiale.…”

Section: Commentairesunclassified

Étude de faisabilité d’un greffon biofabriqué pour traiter des récessions parodontales

Smirani

Delattre

Kérourédan

et al. 2016

Med Buccale Chir Buccale

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Résumé -Introduction : L'ingénierie tissulaire permet d'envisager de nouvelles thérapeutiques pour le traitement des récessions gingivales. Cette étude de faisabilité proposait un protocole et une chronologie pour la biofabrication de greffons parodontaux sur mesure palliant ces défauts. Technique : L'impression d'une matrice en hydrogel collagénique a été réalisée grâce à un système d'éjection piézoélectrique Microdrop®. La mise au point des conditions optimales d'impression a été déterminée en fabriquant des échantillons de dimension et de morphologie compatibles avec une application clinique. L'ensemencement cellulaire de matrices a été réalisé par bioimpression assistée par laser, et la viabilité cellulaire post-impression testée. L'impression d'une matrice a été directement réa-lisée sur les membranes de collagène préfabriquées pour évaluer la facilité de manipulation du greffon. Les matrices obtenues ont la forme souhaitée, les cellules sont viables, et le greffon est aisément manipulable et suturable. Commentaires : L'impression de la matrice permet de choisir l'épaisseur du greffon. Il est attendu in vivo que l'organisation spatiale des fibroblastes au sein des greffons permette d'augmenter la résistance mécanique et l'esthétique des greffes parodontales. Conclusion : Cette étude de faisabilité préliminaire a permis de poser le concept. D'autres études seront nécessaires pour étudier le remodelage du greffon in vitro et in vivo.Abstract -Feasibility study of biofabricated graft for treating periodontal recession. Introduction: Tissue engineering is a good candidate for treating periodontal recession. This feasibility study proposed a protocol for biofabrication of customized periodontal grafts to treat this problem. Technics: Cell seeding of collagen matrices was done by laser-assisted bioprinting, and post-printing cell viability was assessed. The collagen hydrogel matrix printing was performed by a Microdrop® piezoelectric inkjet system. Optimal conditions were determined by fabricating samples whose dimensions and morphology were suitable for clinical use. Then, the hydrogel matrix printing was carried out directly on collagen membranes to evaluate the ease of the graft handling. The hydrogel matrices had the expected shape, the cells were viable, and the graft was easy to handle and suturable. Comments: The matrix printing allowed one to choose the graft thickness. Fibroblast organization was expected to improve the mechanical resistance and esthetics of periodontal grafts in vivo. Conclusion: This preliminary feasibility study established the bases of the concept. More studies are necessary to assess the graft remodeling both in vitro and in vivo.

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Section: Commentairesunclassified

Étude de faisabilité d’un greffon biofabriqué pour traiter des récessions parodontales

Smirani

Delattre

Kérourédan

et al. 2016

Med Buccale Chir Buccale

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“…LIFT is an established technique for cell deposition, with postprinting cell viability reaching > 95% [40,41]. Furthermore, previous studies showed that cell suspensions could withstand laser-induced acceleration and deceleration of the order of 10 6 −10 7 g and authors speculated that only the cells at the front of the jet were damaged [42,43].…”

Section: Application To Particle Deliverymentioning

confidence: 99%

Depth-controlled laser-induced jet injection for direct three-dimensional liquid delivery

et al. 2018

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Needle-free jet injection enables the delivery of drugs into skin or soft tissue by puncturing them with a high-velocity liquid jet. However, precise and efficient drug delivery requires generating such liquid jets with both a controlled velocity and a high throughput, which remains challenging with current spring-and gas-actuated jet injectors. Here, we propose a depth-controlled and high-throughput injection method by adapting laser-induced forward transfer (LIFT), a high-resolution two-dimensional printing technique, for direct three-dimensional liquid delivery into soft tissues.The velocity of thin liquid jets is laser actuated from 10 to 85 m/s so that doses as small as 10 pL, not achievable with other injectors, are injected at a 1 Hz repetition rate into a 300 μm thick soft gelatin substrate with a 25 μm depth precision and 12 μm lateral resolution. We further investigate the potential of this liquid delivery technique as a direct three-dimensional cell-delivery vehicle and show that depth-controlled particle delivery requires high-delivery efficiency. Our direct three-dimensional liquid delivery system opens up more possibilities for pinpoint drug delivery in soft tissues or tissue-engineered constructs.

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“…Laser-assisted bioprinting also enables a great control over the droplet size and number of printed cells per droplet via manipulation of the laser pulse energy, the laser spot size, the distance between the ribbon and the substrate, and the thickness of both energy absorbing layer and cell support layer [53,57,59,89]. Several works have also shown the ability of laser-assisted bioprinting to print mammalian cells without affecting their viability or function, or inducing DNA damage [14,53,55,58,60,89,90,200,203].…”

Section: Laser-assisted Bioprintingmentioning

confidence: 99%

“…For tissue engineering applications, traditional LIFT apparatus has been modified mostly regarding the print ribbon and receiving substrate in order to improve the compatibility with biological materials. The bioink is coated onto the bottom side of the laser transparent substrate and consists in cells suspended in a liquid medium or in a viscous polymer solution [57,58,134]. A high-powered laser pulse (usually a near infra-red laser) is focused onto a thin metal layer (1-100 nm), placed above the laser transparent substrate, generating a high-pressure bubble that propels the material towards a parallel substrate [14,57,128].…”

Section: Laser-assisted Bioprintingmentioning

confidence: 99%

Advances in bioprinted cell-laden hydrogels for skin tissue engineering

et al. 2017

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Bioprinting technologies are powerful additive biofabrication techniques to produce cellular constructs for skin tissue engineering owing to their unique ability to precisely pattern living and non-living materials in predefined spatial locations. This unique feature, combined with the computer controlled printing and medical imaging techniques, enable researchers and clinicians to generate patient specific constructs partly replicating the intricate compositional and architectural organization of the skin. Bioprinting has been used to automatically dispense hydrogels with skin cells located in prescribed sites that promote skin formation in vitro and in vivo. Current skin bioprinting approaches mostly rely on the sequential printing of fibroblasts and keratinocytes embedded within a homogeneous hydrogel. Although such approaches have already been translated to pre-clinical scenarios, they still present limitations in terms of fully replicating the cellular and extracellular matrix (ECM) heterogeneity in native skin. The success of bioprinting for skin repair strongly depends on the design of printable bioinks capable of supporting the function of printed cells and stimulating the production of new ECM components. To better mimic the human skin, novel developments in dedicated bioprinting technologies, in the design of bioinks, as well as in the printing of vascularised constructs are necessary. This paper presents an overview regarding the use of bioprinting for skin tissue engineering applications. The operating principles of bioprinting technologies are outlined along with requirements of printed skin constructs. Finally, preclinical results are summarized and future perspectives for the field are highlighted.

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High-throughput laser printing of cells and biomaterials for tissue engineering

Cited by 417 publications

References 30 publications

Étude de faisabilité d’un greffon biofabriqué pour traiter des récessions parodontales

Étude de faisabilité d’un greffon biofabriqué pour traiter des récessions parodontales

Depth-controlled laser-induced jet injection for direct three-dimensional liquid delivery

Advances in bioprinted cell-laden hydrogels for skin tissue engineering

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