High-throughput metabolomics enables metabolite biomarkers and metabolic mechanism discovery of fish in response to alkalinity stress

Sun, Yanchun; Wu, Song; Du, Ningning; Song, Yi; Xu, Wei

doi:10.1039/c8ra01317a

Cited by 29 publications

(12 citation statements)

References 31 publications

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“…While the exact mechanism for this difference in metabolites across species is not known, there are a few possible mechanisms that could explain this trend. For example, the reduction in these metabolites may be linked to an increased energy demand in the livers of silver carp related to the processing of contaminants [ 94 , 95 , 99 ]. Alternatively, gill raker morphology of these two species differs, which enables silver carp to feed on smaller particles relative to bigheaded carp [ 100 , 101 ] arguably making silver carp a superior competitor [ 29 ], such that differences in liver metabolites may be driven by differences in food when these species are found sympatrically.…”

Section: Discussionmentioning

confidence: 99%

Why the Stall? Using metabolomics to define the lack of upstream movement of invasive bigheaded carp in the Illinois River

Curtis-Quick

Ulanov

et al. 2021

PLoS ONE

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Bigheaded Carp have spread throughout the Mississippi River basin since the 1970s. Little has stopped the spread as carp have the ability to pass through locks and dams, and they are currently approaching the Great Lakes. However, the location of the leading edge in the Illinois River has stalled for over a decade, even though there is no barrier preventing further advancement towards the Great Lakes. Defining why carp are not moving towards the Great Lakes is important for predicting why they might advance in the future. The aim of this study was to test the hypothesis that anthropogenic contaminants in the Illinois River may be playing a role in preventing further upstream movement of Bigheaded Carp. Ninety three livers were collected from carp at several locations between May and October of 2018. Liver samples were analyzed using gas chromatography-mass spectrometry in a targeted metabolite profiling approach. Livers from carp at the leading edge had differences in energy use and metabolism, and suppression of protective mechanisms relative to downstream fish; differences were consistent across time. This body of work provides evidence that water quality is linked to carp movement in the Illinois River. As water quality in this region continues to improve, consideration of this impact on carp spread is essential to protect the Great Lakes.

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Section: Discussionmentioning

confidence: 99%

Why the Stall? Using metabolomics to define the lack of upstream movement of invasive bigheaded carp in the Illinois River

Curtis-Quick

Ulanov

et al. 2021

PLoS ONE

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“…There are six major families of proteins involved in salivary proteomics: proline-rich proteins (PRPs) including acidic PRP(aPRP), basic PRP (bPRP) and glycosylated PRP(gPRP), α-amylases, mucins, salivary (S-type) cystatins, histatins and statherin (Figure 1) (Castagnola et al, 2012a;Schulz et al, 2013a). Although some of their concentrations are 10-15 times lower than protein concentrations in plasma, the advancements in analytical highthroughput technology has greatly increased the detection sensitivity and specificity of proteomic biomarkers in saliva ( Sivadasan et al, 2015;Liang et al, 2016;Sun et al, 2018).…”

Section: Composition Of the Salivary Proteomicsmentioning

confidence: 99%

“…but some salivary proteins with specific roles are only produc ornejo et al, 2012). There are six major families of prot proteomics: proline-rich proteins (PRPs) including acidic P PRP) and glycosylated PRP(gPRP), α-amylases, mucins, s, histatins and statherin (Figure 1) (Castagnola et al, 2012 Although some of their concentrations are 10-15 times lo trations in plasma, the advancements in analytical high-throug atly increased the detection sensitivity and specificity of prote a ( Sivadasan et al, 2015;Liang et al, 2016;Sun et al, 2018).…”

Section: Composition Of the Salivary Proteomicsmentioning

confidence: 99%

A Review on Salivary Proteomics for Oral Cancer Screening

Li¹,

Ouyang²,

Chen³

et al. 2020

Current Issues in Molecular Biology

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Oral cancer has emerged as a global health problem due to its relatively high incidence and mortality. Human saliva as a diagnostic fluid can offer an easy, inexpensive, safe and non-invasive approach for disease detection. Direct contact between saliva and oral cancer lesions make detection of salivary biomarkers for oral cancer especially attractive. Proteins are important molecules involved in pathological processes of oral cancer growth, apoptosis and metastasis. Proteins such as hormones, antibodies, enzymes and cytokines in saliva secreted by oral cancer cells or by host cells not only provide comprehensive pathological information of oral cancer but also are considered potential targets for non-invasive screening of oral cancer. This article provides a review of potential salivary proteomic biomarkers in oral cancer screening.

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“…In the past several years, high-throughput mass spectrometry (MS)-based metabolomics and sequencing techniques have been used to identify the responses of freshwater fish species exposed to different concentrations of saline-alkali stress. For Crucian carp ( Carassius carassius ), the metabolic changes in 7 key pathways, which include phenylalanine metabolism, glycine, serine and threonine metabolism, pyruvate metabolism, tyrosine metabolism, cysteine and methionine metabolism, aminoacyl-tRNA biosynthesis and butanoate metabolism, played a role in adaptation to extremely high alkalinity ( 14 ). For Nile tilapia ( Oreochromis niloticus ) in response to alkalinity stress, comparative transcriptome analysis revealed that the most significant pathway was energy metabolism ( 15 ).…”

Section: Introductionmentioning

confidence: 99%

Effects and Molecular Regulation Mechanisms of Salinity Stress on the Health and Disease Resistance of Grass Carp

Fang

Yang

et al. 2022

Front. Immunol.

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Though some freshwater fish have been successfully cultivated in saline-alkali water, the survival rates of freshwater fish are greatly affected by different saline-alkali conditions. The mechanisms of immune adaptation or immunosuppression of freshwater fish under different saline-alkali stress remain unclear. Here, grass carp were exposed to 3‰ and 6‰ salinity for 30 days. It was observed that salinity treatments had no obvious effects on survival rates, but significantly increased the percent of unhealthy fish. Salinity treatments also increased the susceptibility of grass carp against Flavobacterium columnare infection. The fatality rate (16.67%) of grass carp treated with 6‰ salinity was much lower than that treated with 3‰ salinity (40%). In the absence of infection, higher numbers of immune-related DEGs and signaling pathways were enriched in 6‰ salinity-treated asymptomatic fish than in 3‰ salinity-treated asymptomatic fish. Furthermore different from salinity-treated symptomatic fish, more DEGs involved in the upstream sensors of NOD-like receptor signaling pathway, such as NLRs, were induced in the gills of 6‰ salinity-treated asymptomatic fish. However in the case of F. columnare infection, more immune-related signaling pathways were impaired by salinity treatments. Among them, only NOD-like receptor signaling pathway was significantly enriched at early (1 and/or 2 dpi) and late (7 dpi) time points of infection both for 3‰ salinity-treated and 6‰ salinity-treated fish. Besides the innate immune responses, the adaptive immune responses such as the production of Ig levels were impaired by salinity treatments in the grass carp infected with F. columnare. The present study also characterized two novel NLRs regulated by salinity stress could inhibit bacterial proliferation and improve the survival rate of infected cells. Collectively, the present study provides the insights into the possible mechanisms why the percent of unhealthy fish in the absence of infection and mortality of grass carp in the case of F. columnare infection were much lower in the 6‰ salinity-treated grass carp than in 3‰ salinity-treated grass carp, and also offers a number of potential markers for sensing both environmental salinity stress and pathogen.

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High-throughput metabolomics enables metabolite biomarkers and metabolic mechanism discovery of fish in response to alkalinity stress

Abstract: High throughput mass spectrometry (MS)-based metabolomics is a popular platform for small molecule metabolites analyses that are widely used for detecting biomarkers in the research field of environmental assessment.

Cited by 29 publications

References 31 publications

Why the Stall? Using metabolomics to define the lack of upstream movement of invasive bigheaded carp in the Illinois River

Why the Stall? Using metabolomics to define the lack of upstream movement of invasive bigheaded carp in the Illinois River

A Review on Salivary Proteomics for Oral Cancer Screening

Effects and Molecular Regulation Mechanisms of Salinity Stress on the Health and Disease Resistance of Grass Carp

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