High-throughput microarray for antimicrobial susceptibility testing

Srinivasan, Anand; Lee, Grace C.; Torres, Nelson S.; Hernandez, Kevin; Dallas, Steven D.; Lopez-Ribot, José L.; Frei, Christopher R.; Ramasubramanian, Anand K.

doi:10.1016/j.btre.2017.10.004

Cited by 6 publications

(5 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Various attempts have been made to create bacteria immobilization platforms for performing highly parallelized and automated AST. A nanoarray of spotted antimicrobial-impregnated hydrogel was devised on which microbial samples could be layered . After incubation, the array could be stained and imaged to determine antimicrobial susceptibility.…”

Section: Emerging Innovative Sensing Technologies For Amr Managementmentioning

confidence: 99%

“…A nanoarray of spotted antimicrobial- impregnated hydrogel was devised on which microbial samples could be layered. 56 After incubation, the array could be stained and imaged to determine antimicrobial susceptibility. Similarly, hydrogel immobilization AST platforms (Figure 1B) have been developed to allow for automatic monitoring of microbial growth in response to antimicrobial challenge.…”

Section: ■ Emerging Innovative Sensing Technologies For Amr Managementmentioning

confidence: 99%

See 1 more Smart Citation

Progressing Antimicrobial Resistance Sensing Technologies across Human, Animal, and Environmental Health Domains

et al. 2021

View full text Add to dashboard Cite

The spread of antimicrobial resistance (AMR) is a rapidly growing threat to humankind on both regional and global scales. As countries worldwide prepare to embrace a One Health approach to AMR management, which is one that recognizes the interconnectivity between human, animal, and environmental health, increasing attention is being paid to identifying and monitoring key contributing factors and critical control points. Presently, AMR sensing technologies have significantly progressed phenotypic antimicrobial susceptibility testing (AST) and genotypic antimicrobial resistance gene (ARG) detection in human healthcare. For effective AMR management, an evolution of innovative sensing technologies is needed for tackling the unique challenges of interconnected AMR across various and different health domains. This review comprehensively discusses the modern state-of-play for innovative commercial and emerging AMR sensing technologies, including sequencing, microfluidic, and miniaturized point-of-need platforms. With a unique view toward the future of One Health, we also provide our perspectives and outlook on the constantly changing landscape of AMR sensing technologies beyond the human health domain.

show abstract

Section: Emerging Innovative Sensing Technologies For Amr Managementmentioning

confidence: 99%

Section: ■ Emerging Innovative Sensing Technologies For Amr Managementmentioning

confidence: 99%

Progressing Antimicrobial Resistance Sensing Technologies across Human, Animal, and Environmental Health Domains

et al. 2021

View full text Add to dashboard Cite

show abstract

“…As in the case of C. albicans , the resulting miniaturized bacterial biofilms demonstrated characteristics similar to those displayed by conventionally formed macroscopic biofilms for the two species, including architectural features (Figure 2), synthesis of biofilm matrices, and high levels of resistance to antibiotics [30]. In an extension of these studies, most recently we demonstrated the applicability of a similar technique for culturing community acquired methicillin resistant Staphylococcus aureus (CA-MRSA), and perform antibiotic susceptibility testing against clinical isolates of CA-MRSA, which required significantly smaller sample volumes compared to conventional methods and resulted in much faster results [31].…”

Section: Expansion To Bacterial and Polymicrobial Biofilmsmentioning

confidence: 99%

Nano-biofilm Arrays as a Novel Universal Platform for Microscale Microbial Culture and High-Throughput Downstream Applications

Srinivasan¹,

Ramasubramanian

Lopez-Ribot

2019

CMC

Self Cite

View full text Add to dashboard Cite

Biofilms are the predominant mode of microbial growth and it is now fully accepted that a majority of infections in humans are associated with a biofilm etiology. Biofilms are defined as attached and structured microbial communities surrounded by a protective exopolymeric matrix. Importantly, sessile microorganisms growing within a biofilm are highly resistant to antimicrobial agents. Thus, there is an urgent need to develop new and improved anti-biofilm therapies. Unfortunately, most of the current techniques for in vitro biofilm formation are not compatible with high throughput screening techniques that can speed up discovery of new drugs with anti-biofilm activity. To try to overcome this major impediment, our group has developed a novel technique consisting of micro-scale culture of microbial biofilms on a microarray platform. Using this technique, hundreds to thousands of microbial biofilms, each with a volume of approximately 30–50 nanolitres, can be simultaneously formed on a standard microscope slide. Despite more than three orders of magnitude of miniaturization over conventional biofilms, these nanobiofilms display similar growth, structural and phenotypic properties, including antibiotic drug resistance. These nanobiofilm chips are amenable to automation, drastically reducing assay volume and costs. This technique platform allows for true high-throughput screening in search for new anti-biofilm drugs.

show abstract

“…Spontaneous aggregation driven by low-shear conditions either in a shake flask or by magnetic levitation also produces biofilm-like morphological and drug susceptibility characteristics ( 13 , 14 ). We have shown that biofilms of single and polymicrobial nanocultures of Candida albicans , S. aureus , and P. aeruginosa encapsulated in alginate or collagen grow as dense microcolonies, express copious exopolymeric matrix, and show increased antibiotic resistance ( 15 – 17 ). Our nanoculture microarray platform not only facilitated ultrahigh-throughput screening of antibiofilm drugs but also prompted insights into the possibility of biofilm formation in three dimensions instead of traditional, gravity-driven surface attachment in two dimensions as a prerequisite first step.…”

Section: Introductionmentioning

confidence: 99%

A Facile High-Throughput Model of Surface-Independent Staphylococcus aureus Biofilms by Spontaneous Aggregation

Cheng

Torres

Chen

et al. 2021

mSphere

Self Cite

View full text Add to dashboard Cite

The canonical model of biofilm formation begins with the attachment and growth of microbial cells on a surface. While these in vitro models reasonably mimic biofilms formed on foreign bodies such as catheters and implants, this is not the case for biofilms formed in cystic fibrosis and chronic wound infections, which appear to present as aggregates not attached to a surface.

show abstract

High-throughput microarray for antimicrobial susceptibility testing

Abstract: HighlightsDeveloped a high-throughput microarray for anti-microbial susceptibility testing (AST).Demonstrated that the feasibility of the AST against clinical isolates of MRSA.Platform is a low sample volume, rapid, high-throughput alternative to traditional assays.

Cited by 6 publications

References 15 publications

Progressing Antimicrobial Resistance Sensing Technologies across Human, Animal, and Environmental Health Domains

Progressing Antimicrobial Resistance Sensing Technologies across Human, Animal, and Environmental Health Domains

Nano-biofilm Arrays as a Novel Universal Platform for Microscale Microbial Culture and High-Throughput Downstream Applications

A Facile High-Throughput Model of Surface-Independent Staphylococcus aureus Biofilms by Spontaneous Aggregation

Contact Info

Product

Resources

About