High-throughput microfluidic device for rare cell isolation

Yang, Daniel; Leong, Serena; Lei, Andy; Sohn, Lydia L.

doi:10.1117/12.2178613

Cited by 8 publications

(10 citation statements)

References 11 publications

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“…We next challenged the device with a mixture of 7.32 μm, 15.45 μm and 26.3 μm diameter beads with the resistance ratio of σ = 0.59. Mixtures of two or three particle sizes are typically used to define cutoff sizes of a microfluidic device to prepare for biological sample [18,20,21,37]. Using a mixture also allowed for considerations of particle-on-particle interactions because of higher concentrations in the channel.…”

Section: Application To Cell Cycle Synchronizationmentioning

confidence: 99%

Evaluation of Performance and Tunability of a Co-Flow Inertial Microfluidic Device

2020

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Microfluidics has gained a lot of attention for biological sample separation and purification methods over recent years. From many active and passive microfluidic techniques, inertial microfluidics offers a simple and efficient method to demonstrate various biological applications. One prevalent limitation of this method is its lack of tunability for different applications once the microfluidic devices are fabricated. In this work, we develop and characterize a co-flow inertial microfluidic device that is tunable in multiple ways for adaptation to different application requirements. In particular, flow rate, flow rate ratio and output resistance ratio are systematically evaluated for flexibility of the cutoff size of the device and modification of the separation performance post-fabrication. Typically, a mixture of single size particles is used to determine cutoff sizes for the outlets, yet this fails to provide accurate prediction for efficiency and purity for a more complex biological sample. Thus, we use particles with continuous size distribution (2–32 μm) for separation demonstration under conditions of various flow rates, flow rate ratios and resistance ratios. We also use A549 cancer cell line with continuous size distribution (12–27 μm) as an added demonstration. Our results indicate inertial microfluidic devices possess the tunability that offers multiple ways to improve device performance for adaptation to different applications even after the devices are prototyped.

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Section: Application To Cell Cycle Synchronizationmentioning

confidence: 99%

Evaluation of Performance and Tunability of a Co-Flow Inertial Microfluidic Device

2020

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“…Contraction-expansion-array channel (CEA channel) inertial microfluidics [ 62 , 63 , 64 ] is the deformation of a straight channel through adding or subtracting a specific shape to form a cavity in a straight channel. The expansion structure increases the control flexibility by the section vortex.…”

Section: Progress In Inertial Microfluidicsmentioning

confidence: 99%

Progress of Inertial Microfluidics in Principle and Application

Gou

Jia

Wang

et al. 2018

Sensors

137

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Inertial microfluidics has become a popular topic in microfluidics research for its good performance in particle manipulation and its advantages of simple structure, high throughput, and freedom from an external field. Compared with traditional microfluidic devices, the flow field in inertial microfluidics is between Stokes state and turbulence, whereas the flow is still regarded as laminar. However, many mechanical effects induced by the inertial effect are difficult to observe in traditional microfluidics, making particle motion analysis in inertial microfluidics more complicated. In recent years, the inertial migration effect in straight and curved channels has been explored theoretically and experimentally to realize on-chip manipulation with extensive applications from the ordinary manipulation of particles to biochemical analysis. In this review, the latest theoretical achievements and force analyses of inertial microfluidics and its development process are introduced, and its applications in circulating tumor cells, exosomes, DNA, and other biological particles are summarized. Finally, the future development of inertial microfluidics is discussed. Owing to its special advantages in particle manipulation, inertial microfluidics will play a more important role in integrated biochips and biomolecule analysis.

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“…The flow rate, sample concentration, and device geometry are usually the key factors for the successful separation using this method. However, the overlap in physical properties of the target and non-target cells interferes with the separation purity (Yang, Leong andSohn 2015, Ryu et al 2017).…”

Section: Passive Cell Separation Techniquesmentioning

confidence: 99%