High-Throughput Mosquito and Fly Bioassay System for Natural and Artificial Substrates Treated with Residual Insecticides

Aldridge, Robert L.; Wynn, W. Wayne; Britch, Seth C.; Allan, Sandra A.; Walker, Todd W.; Geden, Christopher J.; Hogsette, Jerome A.; Linthicum, Kenneth J.

doi:10.2987/12-6307r.1

Cited by 5 publications

(4 citation statements)

References 11 publications

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“…Similar analyses between treated and untreated enclosures with each spatial repellent present also showed no statistical differences. Subsequent laboratory analysis of cuttings taken the day of the residual pesticide treatment of the ULCANS enclosures using the bioassay process described in Aldridge et al (2013) showed a mean effect of only 30.2% corrected 24-h mortality (Abbott 1925) on susceptible colony-reared Cx. quinquefasciatus.…”

mentioning

confidence: 99%

Spatial Repellents Protect Small Perimeters from Riceland Mosquitoes in a Warm-Humid Environment

Britch¹,

Dame²,

Meisch³

et al. 2021

Journal of the American Mosquito Control Association

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Recent experiments suggest spatial repellents may significantly reduce biting pressure from host-seeking riceland mosquitoes, such as Anopheles quadrimaculatus, in a warm-humid open-field habitat. However, little is known regarding efficacy of these formulations in partially enclosed spaces where US military personnel may be sheltered or concealed in an operational environment. In this study we investigated the capability of 3 spatial repellents—metofluthrin, linalool, and d-cis/trans allethrin—to reduce mosquito incursion into small open-top enclosures of US military camouflage netting. We found that metofluthrin was more effective in partially enclosed spaces compared with the open field, whereas both linalool and d-cis/trans allethrin provided superior protection in the open. These findings support strategic selection of spatial repellents depending on the environment immediately surrounding the host.

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confidence: 99%

Spatial Repellents Protect Small Perimeters from Riceland Mosquitoes in a Warm-Humid Environment

Britch¹,

Dame²,

Meisch³

et al. 2021

Journal of the American Mosquito Control Association

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“…First, we placed a CDC trap baited with light and CO 2 (dry ice) for 24 hr in the center of the protected space in all 16 HESCO perimeters to periodically survey the overnight penetration of the perimeters by natural populations of medically important mosquitoes. Second, at the time of mosquito collection we cut ~15 cm x 15 cm samples of geotextile from the inside and outside facing surfaces of each perimeter and exposed them to laboratory colony Culex quinquefasciatus females in glass tube bioassays at the USDA Agricultural Research Service Center for Medical, Agricultural, and Veterinary Entomology (CMAVE) following the methods and colony description of Aldridge et al (2012Aldridge et al ( , 2013 and Britch et al (2009Britch et al ( , 2010Britch et al ( , 2011. We stored each cutting separately in labeled re-sealable bags to minimize opportunity for cross-contamination of samples.…”

Section: B Amentioning

confidence: 99%

EARTH FILL INCREASES EFFICACY AND LONGEVITY OF Λ-Cyhalothrin RESIDUAL INSECTCIDE TREATMENT OF HESCO® BLAST WALL GEOTEXTILE

Britch¹

2021

J FL Mosq Control Assoc

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The prevention of vector-borne disease to protect the health and readiness of United States forces in the field continues to be a high priority for the US Department of Defense. Previous studies have demonstrated that the risk of human contact with disease-vector mosquitoes and other biting flies can be reduced by applying an insecticide to perimeters of military materials such as camouflage netting or HESCO blast protection wall geotextile already in place around troops in the field. In this study we investigated whether residual pesticide efficacy will persist in the presence of earth fill that is required for operational use of HESCOs, using a warm temperate field site in north Florida. Results from laboratory bioassays measuring mosquito mortality and field collections of natural mosquito populations indicated superior efficacy and greater longevity of pesticide treated geotextile exposed to soil fill. These findings not only support immediate implementation of this technique in US military field scenarios, but also provide evidence that HESCO technology currently used in natural disaster flood control could be leveraged to protect civilian personnel from emerging floodwater mosquitoes.

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“…Set the timer for 4 min, which is the approximate time before mosquitoes will become active again. Use a pistol aspirator 15,16 or soft-touch insect forceps to pick out batches of 20 adult females and immediately place them in the waiting open sentinel cages. Once all females have been picked out of the anaesthetized batch, use a separate mechanical aspirator to collect all remaining male mosquitoes which will be destroyed by freezing.…”

Section: Sentinel Insect Rearing and Sentinel Cage Preparationsmentioning

confidence: 99%

Visualizing Efficacy of Pesticides Against Disease Vector Mosquitoes in the Field

Britch

Linthicum

Aldridge

et al. 2019

JoVE

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Efficacy of public health pesticides targeting nuisance and disease-vector insects such as mosquitoes, sand flies, and filth-breeding flies is not uniform across ecological zones. To best protect public and veterinary health from these insects, the environmental limitations of pesticides need to be investigated to inform effective use of the most appropriate pesticide formulations and techniques. We have developed a research program to evaluate combinations of pesticides, pesticide application equipment, and application techniques in hot-arid desert, hot-humid tropical, warm and cool temperate, and urban locations to derive pesticide use guidelines specific to target insect and environment. To these ends we designed a system of protocols to support efficient, cost-effective, portable, and standardized evaluation of a diverse range of pesticides and equipment across multiple environments. At the core of these protocols is the use of an array of small cages with colony-reared sentinel mosquitoes (adults and immatures) and sand flies (adults), strategically arranged in natural habitats and exposed to pesticide spray. Spatial and temporal patterns of pesticide efficacy are derived from percent mortality in sentinel cages, then mapped and visualized in a geographic information system. Maps of sentinel mortality data may be statistically compared to evaluate relative efficacy of a pesticide across multiple environments, or to study multiple pesticides in a single environment. Protocols may be modified to accommodate a variety of scenarios, including, for example, the vertical orientation of sentinels in canopy habitats or simultaneous testing of ground and aerial application methods.

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High-Throughput Mosquito and Fly Bioassay System for Natural and Artificial Substrates Treated with Residual Insecticides

Cited by 5 publications

References 11 publications

Spatial Repellents Protect Small Perimeters from Riceland Mosquitoes in a Warm-Humid Environment

Spatial Repellents Protect Small Perimeters from Riceland Mosquitoes in a Warm-Humid Environment

EARTH FILL INCREASES EFFICACY AND LONGEVITY OF Λ-Cyhalothrin RESIDUAL INSECTCIDE TREATMENT OF HESCO® BLAST WALL GEOTEXTILE

Visualizing Efficacy of Pesticides Against Disease Vector Mosquitoes in the Field

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