High-Throughput PET/CT Imaging Using a Multiple-Mouse Imaging System

Greenwood, Hannah E.; Nyitrai, Z.; Mócsai, Gábor; Hobor, Sandor; Witney, Timothy H.

doi:10.2967/jnumed.119.228692

Cited by 34 publications

(38 citation statements)

References 13 publications

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“…Similar behavior has been observed and reported on by Aide et al [32] and Siepel et al [9], who attributed this to the combined effect of 'over-estimation of scatter correction plus the nonnegativity constraint in the image reconstruction'. Furthermore, Greenwood et al [12] showed that the smaller, 1 mm diameter rod was not discernible when scanning four phantoms simultaneously, which stands by our observations that RC diminishes as more sources of activity are introduced into the FOV.…”

Section: Recovery Coefficientsmentioning

confidence: 51%

“…Reilhac et al [11] reported a higher increase of 43% in the %STD of the uniformity when scanning a Derenzo phantom alongside a NEMA IQ phantom. Finally, Greenwood et al [12] reported a decrease in uniformity (an increase of 55% in %STD) when assessing four phantoms simultaneously vs the single centered case. It may be expected to see such a significant increase in %STD when there are four sources of activity present, which was not assessed in our study.…”

Section: Uniformitymentioning

confidence: 99%

“…Most recently, Greenwood et al [12] characterized a commercial four-chamber bed on a Mediso preclinical PET scanner, using NEMA phantoms and mice. As in previous studies, they demonstrated a decrease in uniformity and recovery coefficients, and an increase in spillover ratios, in the multiple vs. single animal cases, though these increases were within the recently suggested limits for bias [13].…”

Section: Introductionmentioning

confidence: 99%

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Influence of Multiple Animal Scanning on Image Quality for the Sedecal SuperArgus2R Preclinical PET Scanner

et al. 2021

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Background: Increased throughput in small animal preclinical studies using positron emission tomography leads to reduced costs and improved efficiency of experimental design, however the presence of multiple off-centre subjects, as opposed to a single centered one, may affect image quality in several ways.Methods: We evaluated the count rate performance using a NEMA scatter phantom. A Monte Carlo simulation of the system was validated against this dataset and used to simulate the count rate performance for dual scatter phantoms. NEMA NU4 image quality phantoms were then scanned in the central and offset positions, as well as in the offset position next to a uniform activity phantom. Uniformity, recovery coefficients and spillover ratios were then compared, as were two time frames for acquisition.Results: Count rate performance assessed with a single NEMA scatter phantom was in line with previous literature, with simulated data in good agreement. Simulation of dual scatter phantoms showed an increase in scatter fraction. For the NEMA Image Quality phantom, uniformity and Recovery coefficients were degraded in the offset, and dual phantom cases, while spillover ratios were increased, notably when the chamber was placed nearest the gantry. Image quality metrics were comparable between the 20- and 10 min timeframes.Conclusion: Dual animal scanning results in some loss of image quality on the Sedecal Argus PET scanner; however, this degradation is within acceptable limits.

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Section: Recovery Coefficientsmentioning

confidence: 51%

Section: Uniformitymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Influence of Multiple Animal Scanning on Image Quality for the Sedecal SuperArgus2R Preclinical PET Scanner

et al. 2021

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“…We encourage the reader to refer elsewhere [1][2][3] for a detailed discussion of resolution and image quality obtainable with standard radionuclides (e.g. 18 F, 11 C) in simultaneous multi-animal imaging.…”

Section: Discussionmentioning

confidence: 99%

3D-Printable Platform for High-Throughput Small-Animal Imaging

et al. 2020

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There is considerable interest in preclinical imaging techniques to increase throughput and reduce costs, as evidenced by recent and former publications in the field. 1-3 We write this letter to share a 3D-printable design that has been in use at our institution and several others for small animal PET/CT, and which we feel would likewise benefit the community, particularly at facilities that encourage DIY approaches. Our design is inexpensively manufactured with 3D-printers that are increasingly accessible at many institutions. Due to its compact size, it is easy to integrate with a wide variety of scanner models and apply to various imaging configurations. We have attached the design files in the electronic supplementary information of this letter, along with a short video demonstrating assembly of the components. We highly encourage the reader to watch the video, as it thoroughly details the ease of construction and utility of our design. METHODS The use of this design for murine PET/CT imaging was conducted under the protocol approved by the Institutional Animal Care and Use Committee (IACUC) and Research Animal Resource Center (RARC) of Memorial Sloan Kettering Cancer Center. The design (Fig. 1) was generated in the open-source computer-aided modeling software Blender®, making use of Boolean operations on geometric primitives and a small amount of basic mesh manipulation. Several inexpensive, commercially available components were utilized for connection to an anesthesia system:

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“…One solution to this has been the use of 3D printed beds, referred to as hotel imaging. This allows the researcher to image 2-4 animals at a time (Greenwood et al 2019;Dazai et al 2011). Thanks to their technical and clinical experience, NMTs and radiographers can easily position themselves in this kind of structure.…”

Section: What Is Preclinical Imaging?mentioning

confidence: 99%

Why the preclinical imaging field needs nuclear medicine technologists and radiographers?

Dillenseger

Choquet

Snay

et al. 2020

European J Hybrid Imaging

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Introduction: Preclinical imaging is still seen as a new field, and its recognition as a specific topic occurring only about 20 years ago. Nuclear medicine technologists (NMTs) and radiographers' skills covering technical, anatomical and clinical fields can be highly beneficial to preclinical imaging research centres: many tasks and knowledge are complementary between clinics and preclinical laboratories. Our goal is to reach a consensus on the required set of competencies needed to translate the work of NMTs and radiographers from the clinic to the preclinical laboratory, particularly in regard to multimodal imaging. Preclinical imaging environment: Currently, all imaging modalities used in clinical routine (ultrasound, CT, MRI, PET, SPECT, radiographs) are available, using specific architectures allowing for the spatial resolution and sensitivity needed for small rodents (which are the most commonly used species in research). Ideally, a preclinical laboratory should produce images/examinations at a high throughput in order to meet the statistical expectations of the studies (while respecting the 3R principles for animal research) and the care and welfare of each individual. To reach the quality and throughput expectations of such an organization, specific qualified professionals are needed to complete the scientific/research staff. Where NMTs and radiographers fit in: The increasing use of preclinical imaging requires professionals who can put imaging procedures into action, ensuring a significant success throughput. NMTs and radiographers have a variety of skills that work well within a preclinical laboratory, with the ability to perform the following tasks independently: animal preparation, positioning, monitoring and anaesthesia recovery, acquisition parameter programming, archiving and data processing, device quality controls, surface cleaning and disinfection, radioactive and biological waste management, radiation safety for users, use of hot lab equipment and auxiliary equipment, injected products and material management. In light of the current European Qualification Framework, a set of skills, knowledge and competencies were defined to cover the whole set of duties and tasks deliverable to an NMT or radiographer working in a preclinical laboratory. One of the key responsibilities of the NMT or radiographer is related to compliance on animal care and welfare when undertaking any animal procedures, including imaging.

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High-Throughput PET/CT Imaging Using a Multiple-Mouse Imaging System

Cited by 34 publications

References 13 publications

Influence of Multiple Animal Scanning on Image Quality for the Sedecal SuperArgus2R Preclinical PET Scanner

Influence of Multiple Animal Scanning on Image Quality for the Sedecal SuperArgus2R Preclinical PET Scanner

3D-Printable Platform for High-Throughput Small-Animal Imaging

Why the preclinical imaging field needs nuclear medicine technologists and radiographers?

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